Effect of thermal stress on protein expression in the mussel Mytilus galloprovincialis Lmk

Gonzalez-Riopedre, M.; Novas, A. J.; Dobaño, E.; Ramos-Martı́nez, Juan Ignacio; Barcia, Ramiro

doi:10.1016/j.cbpb.2007.03.006

Cited by 4 publications

(3 citation statements)

References 57 publications

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“…HSF is the master regulator of HSPs in response to heat shock and is evolutionarily conserved in eukaryotes from yeast to mammals (Morimoto, '98; Pirkkala et al, 2001). To determine the ability of marine shellfish to tolerate stress conditions and their potential use as biomarkers, their HSPs and/or HSF have been investigated (Clegg et al, '98; Tomanek and Somero, '99, 2000, 2002; Buckley et al, 2001; Snyder et al, 2001; Piano et al, 2002; Boutet et al, 2003, 2004; Hamdoun et al, 2003; Tomanek, 2005; Farcy et al, 2007, 2009; González‐Riopedre et al, 2007). However, many of these investigations identified HSPs by the use of antibodies against HSPs of mammals or other species, or identified HSPs only by their molecular weights.…”

Section: Discussionmentioning

confidence: 99%

“…In a disrupted cellular environment, molecular chaperones such as HSPs are necessary for the maintenance of cellular homeostasis (Fink, '99; Bukau et al, 2006). In spite of many investigations of molecular chaperones in marine shellfishes (Clegg et al, '98; Tomanek and Somero, '99, 2000, 2002; Buckley et al, 2001; Snyder et al, 2001; Piano et al, 2002; Boutet et al, 2003, 2004; Hamdoun et al, 2003; Tomanek, 2005; Farcy et al, 2007, 2009; González‐Riopedre et al, 2007; Kawabe and Yokoyama, 2009, 2010), the molecular characterization and/or transcriptional/translational regulatory mechanisms of molecular chaperones have not been elucidated. Several reports suggest that the temperature threshold of HSP induction differs with the habitat and/or species (Tomanek and Somero, '99, 2000, 2002; Piano et al, 2002; Tomanek, 2005).…”

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confidence: 99%

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Novel isoforms of heat shock transcription factor 1 are induced by hypoxia in the Pacific oyster Crassostrea gigas

Kawabe

Yokoyama

2011

J. Exp. Zool.

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The Pacific oyster Crassostrea gigas inhabits the intertidal zone and shows tolerance to various stress conditions such as hypoxia and heat shock. However, little is known about the cellular mechanism of responses to these stresses. Heat shock transcription factor 1 (HSF1) regulates the transcription of several genes, including heat shock proteins (HSPs). In this study, we cloned HSF1 from the oyster and investigated its response to air-exposure. The cDNA of oyster Hsf1 contains 2,931 bp, of which 1,389 bp encode a protein of 463 amino acid residues. Moreover, we found that the oyster has seven novel alternatively spliced isoforms, Hsf1b-h, consisting of insertion A with 48 bp, insertion B with 42 bp and/or insertion C with 42 bp. We determined the sequences of oyster genomic DNA containing Hsf1 insertions A, B and C. The results indicated that eight isoforms of Hsf1 are generated from a single Hsf1 gene by alternative splicing without frameshifting. Real-time PCR analysis showed that Hsf1a is expressed constitutively, and the expression of Hsf1b-h and Hsp70 mRNA is induced by air exposure and/or hypoxia. In addition, we found that 11 putative hypoxia response elements, which are hypoxia-inducible factor 1 (HIF-1) binding sequences, are located in the Hsf1 promoter region. These data suggest that the oyster has an HIF-HSF pathway in which HSPs are induced in an HIF-dependent manner, and that it also has a novel mechanism of alternative splicing of Hsf1 in response to hypoxia.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Novel isoforms of heat shock transcription factor 1 are induced by hypoxia in the Pacific oyster Crassostrea gigas

Kawabe

Yokoyama

2011

J. Exp. Zool.

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“…Analysis of heat‐shock protein (Hsp) induction can be a good model to monitor sub‐lethal stress upon an organism (Dalhoff, 2004). Heat shock proteins (Hsp) are a highly conserved class of molecular chaperone that are up‐regulated during periods of stress to repair damaged and denatured proteins (Lindquist and Craig, '88; Hendrick and Hartl, '93; Parsell and Lindquist, '93; Feder and Hofman, '99; Gonzalez‐Riopedre et al, 2007). Heat shock protein induction due to temperature stress has been documented in numerous bivalve species, including Mytilus spp.…”

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confidence: 99%

Thermal constraints for range expansion of the invasive green mussel, Perna viridis, in the southeastern United States

2010

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Cold temperatures are thought to be among the most important determining factors of geographic distribution for tropical and sub-tropical marine invertebrates. The Asian green mussel, Perna viridis, has been introduced into coastal waters of Florida where its current distribution is hypothesized to be limited by low temperatures during winter. Lethal and sub-lethal effects (heat shock protein/Hsp70 expression) of cold water and air temperatures were analyzed in two size classes of P. viridis from Florida in an effort to determine the effects of current and forecasted temperatures on the potential for range expansion. Mussels were exposed to water temperatures of 14, 10, 7 and 3°C for up to 30 days, or to air temperatures of 14, 7, 0 and –10°C for periods of 2 hr. Mortality was significantly increased at all water and air temperatures ≤ 14°C. No differences in mortality rates were observed between small (15–45 mm) and large (75–105 mm) size classes except after exposure to 7°C air, in which small mussels had higher mortality. Significant increases in Hsp70 expression were observed after a 2-hour exposure to 10°C water, but Hsp70 expression was not significantly increased at any temperatures in which mortality was not also significant. The temperature threshold for survival in this population appears to be between 10 and 14°C, suggesting that under current conditions P. viridis may already be at the northern edge of its potential range in the United States. If water temperatures increase with global climate change, northerly flowing currents may permit range expansion as temperatures allow.

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Response of larval barnacle proteome to CO2-driven seawater acidification

Wong

Lane

Leung

et al. 2011

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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Effect of thermal stress on protein expression in the mussel Mytilus galloprovincialis Lmk

Cited by 4 publications

References 57 publications

Novel isoforms of heat shock transcription factor 1 are induced by hypoxia in the Pacific oyster Crassostrea gigas

Novel isoforms of heat shock transcription factor 1 are induced by hypoxia in the Pacific oyster Crassostrea gigas

Thermal constraints for range expansion of the invasive green mussel, Perna viridis, in the southeastern United States

Response of larval barnacle proteome to CO2-driven seawater acidification

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