“…The effects on food products are affected by several factors such as temperature, processing time, frequency, ultrasonic power (Carrillo-Lopez et al, 2021;Scudino et al, 2020). Several previous studies proved that thermosonication causes changes in several food product such as, buffalo milk fat globule size decreases at 20 kHz, 25 °C (Abesinghe et al, 2020), denaturation of sweet whey protein at 20 kHz, 65 °C (Barukčić et al, 2015), increase in cow's milk fat content at 24 kHz, 63 °C (Bermúdez-Aguirre et al, 2008), appearance of burnt off-flavors, 2008), the appearance of burnt off-flavor in milk at 24 kHz, 74 °C (Marchesini et al, 2015), no significant change in the pH of cow's milk at 24 kHz, 55 °C (Juraga et al, 2021), reduce total microbes and can maintain the antioxidant activity of galangal rice at 22 kHz, 60 °C (Safitri et al, 2022). Based on this, this study was conducted to determine the effect of frequency and duration of thermosonication with the addition of moderate heating on the physical, chemical and microbiological quality of cow's milk.…”