Effect of Thiamine Pyrophosphate upon Possible Metamizole-Induced Liver Injury in Rats

Bedir, Zehra; Ozkaloglu, Kezban; Can, Abdullah; Çiçek, Betül; Gülaboğlu, Mine; Süleyman, Zeynep; Gürsul, Cebrail; Mokhtare, Behzad; Özçıçek, Fatih; Süleyman, Halis

doi:10.3923/ijp.2023.139.146

Cited by 4 publications

(2 citation statements)

References 26 publications

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“…The ROS appeared in cells under physiological conditions and were neutralized with antioxidant defense systems 9 . The increase in oxidant levels and decrease in enzymatic and non-enzymatic antioxidants such as GSH, GPO and GSHRd were regarded to be oxidative stress in the literature 25,29 .…”

Section: Discussionmentioning

confidence: 99%

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Effect of Thiamine Pyrophosphate Upon Oxidative Brain Injury Induced by Ischemia-Reperfusion in Rats

Yaşar¹,

Demirdögen²,

Mammadov³

et al. 2023

International J. of Pharmacology

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Background and Objective: Exposure of the brain to Ischemia-Reperfusion (I/R) may cause tissue damage through oxidative stress.Thiamine pyrophosphate (TPP), which has a protective effect against oxidative stress, is the active metabolite of vitamin B 1 . In this study, the protective effect of TPP against possible I/R damage of brain tissue was investigated. Materials and Methods: Thirty rats were randomly divided into BIR, TIR and HG groups consisting of ten rats. In the TIR group, 20 mg kgG 1 TPP was injected intraperitoneally (ip). After 1 hr, clips were placed in the common carotid arteries of the BIR and TIR groups under anesthesia. Brain tissue was subjected to ischemia for 10 min. Afterward, the clips were opened and 3 hrs of reperfusion was achieved. In the HG group, only subcutaneous incisions were made and closed. Then, the brain tissues removed by euthanasia were biochemically analyzed. Results: While, I/R increased malondialdehyde (MDA), myeloperoxidase (MPO), Tumor Necrosis Factor-" (TNF-"), Interleukin-1$ (IL-1$) and 8-Hydroxyguanine (8-OHGua) levels in brain tissues, total caused a decrease in glutathione (tGSH), glutathione peroxidase (GPO) and glutathione reductase (GSHRd) levels (p<0.001). The TPP applied before I/R significantly prevented these changes (p<0.05). Conclusion: The results of biochemical tests suggested that TPP may be beneficial in preventing possible brain damage due to I/R.

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Section: Discussionmentioning

confidence: 99%

“…In this study, thiamine pyrophosphate (TPP), whose effect was tested against oxidative brain I/R damage, is the active metabolite of thiamine (vitamin B 1 ) 9 . TPP was the best indicator specifying the activity level of thiamine 10 and formed by the phosphating of thiamine in the liver with thiamine pyrophosphokinase 11 .…”

Section: Introductionmentioning

confidence: 99%

Effect of Thiamine Pyrophosphate Upon Oxidative Brain Injury Induced by Ischemia-Reperfusion in Rats

Yaşar¹,

Demirdögen²,

Mammadov³

et al. 2023

International J. of Pharmacology

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Adverse effects of metamizole on heart, lung, liver, kidney, and stomach in rats

Ciftel,

Suleyman,

Mammadov

et al. 2024

BMC Pharmacol Toxicol

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Background Metamizole is banned in some countries because of its toxicity, although it is widely used in some European countries. In addition, there is limited information on its safety profile, and it is still debated whether it is toxic to the heart, lungs, liver, kidneys, and stomach. AimsOur study investigated the effects of metamizole on the heart, lung, liver, kidney, and stomach tissues of rats.Methods Eighteen rats were divided into three groups, wassix healthy (HG), 500 mg/kg metamizole (MT-500), and 1000 mg/kg metamizole (MT-1000). Metamizole was administered orally twice daily for 14 days. Meanwhile, the HG group received pure water orally. Biochemical, histopathologic, and macroscopic examinations were performed on blood samples and tissues.Results Malondialdehyde (MDA), total glutathione (tGSH), superoxide dismutase (SOD), and catalase (CAT) in the lung and gastric tissues of MT-500 and MT-1000 groups were almost the same as those of the HG (p > 0.05). However, MDA levels in the heart and liver tissues of MT-500 and MT-1000 groups were higher (p < 0.05) compared to the HG, while tGSH levels and SOD, and CAT activities were lower (p < 0.05). MDA levels of MT-500 and MT-1000 groups in the kidney tissue increased the most (p < 0.001), and tGSH levels and SOD and CAT activities decreased the most (p < 0.001) compared to HG. Metamizole did not cause oxidative damage in the lung and gastric tissue. While metamizole did not change troponin levels, it significantly increased alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), and creatinine levels compared to HG. Histopathologically, mild damage was detected in heart tissue, moderate damage in liver tissue, and severe damage in renal tissue. However, no histopathologic damage was found in any groups' lung and gastric tissues. ConclusionMetamizole should be used under strict control in patients with cardiac and liver diseases and it would be more appropriate not to use it in patients with renal disease.

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Effects of Metamizole, 4-Methylaminoantipyrine, and 4-Aminoantipyrine on LX-2 Liver Cell Line Viability and Apoptosis

Lupu,

Pall,

Cenariu

et al. 2024

Molecules

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Metamizole (dipyrone) is a non-opioid analgesic widely used in human and veterinary medicine, despite ongoing concerns about its safety due to risks such as agranulocytosis and potential hepatotoxicity. This study investigates the cytotoxic (MTT assay) and pro-apoptotic effects of metamizole and its primary metabolites, 4-methylaminoantipyrine (4-MAA) and 4-aminoantipyrine (4-AA), on the LX-2 liver cell line. These metabolites are implicated in both the therapeutic and adverse effects of the drug. The objective is to elucidate the mechanisms of potential hepatotoxicity, with a focus on cell viability and apoptosis. Metamizole was tested at five concentrations (100, 200, 400, 600, and 1000 µg/mL), while its metabolites were tested at two concentrations (100 and 1000 µg/mL). The results show a dose-dependent decrease in cell viability, with significant reductions at higher concentrations. The greatest cytotoxic effects were observed with 4-AA and 4-MAA, which induced marked apoptosis at 1000 µg/mL. This study concludes that metamizole and its metabolites can cause liver cell damage, underscoring the importance of caution in its clinical use and the need for further research to ensure its safety.

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Effect of Thiamine Pyrophosphate upon Possible Metamizole-Induced Liver Injury in Rats

Cited by 4 publications

References 26 publications

Effect of Thiamine Pyrophosphate Upon Oxidative Brain Injury Induced by Ischemia-Reperfusion in Rats

Effect of Thiamine Pyrophosphate Upon Oxidative Brain Injury Induced by Ischemia-Reperfusion in Rats

Adverse effects of metamizole on heart, lung, liver, kidney, and stomach in rats

Effects of Metamizole, 4-Methylaminoantipyrine, and 4-Aminoantipyrine on LX-2 Liver Cell Line Viability and Apoptosis

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