Effect of thuringiensin on adenylate cyclase in rat cerebral cortex

Tsai, Shu-Yao; Yang, Chi; Wang, Shun-Cheng; Wang, Jiunn-Shiow; Hwang, Jenn-Sheng; Ho, Shu-Peng

doi:10.1016/j.taap.2003.08.015

Cited by 16 publications

(7 citation statements)

References 29 publications

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“…The treated rats presented abnormal histology, including disseminated inflammation in the bronchioles and alveoli, bronchial cellular necrosis on days 1 and 2, and areas of septal thickening with cellular infiltration and collagen deposits in the intestinal and alveolar spaces on days 4–5 [20]. Tsai et al (2004) demonstrated that Thu could activate basal adenylate cyclase activity and increase cAMP concentrations in rat cerebral cortex or in a commercial adenylate cyclase [21]. Thu is generally regarded as a weak activator of adenylate cyclase or an inhibitor of forskolin-stimulated adenylate cyclase; therefore, changes in pulmonary oxidative-antioxidative status might have an important role in Thu-induced lung injury (2006) [22].…”

Section: Discussionmentioning

confidence: 99%

Thuringiensin: A Thermostable Secondary Metabolite from Bacillus thuringiensis with Insecticidal Activity against a Wide Range of Insects

Liu

Ruan

Peng

et al. 2014

Toxins

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Thuringiensin (Thu), also known as β-exotoxin, is a thermostable secondary metabolite secreted by Bacillus thuringiensis. It has insecticidal activity against a wide range of insects, including species belonging to the orders Diptera, Coleoptera, Lepidoptera, Hymenoptera, Orthoptera, and Isoptera, and several nematode species. The chemical formula of Thu is C22H32O19N5P, and it is composed of adenosine, glucose, phosphoric acid, and gluconic diacid. In contrast to the more frequently studied insecticidal crystal protein, Thu is not a protein but a small molecule oligosaccharide. In this review, a detailed and updated description of the characteristics, structure, insecticidal mechanism, separation and purification technology, and genetic determinants of Thu is provided.

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Section: Discussionmentioning

confidence: 99%

Thuringiensin: A Thermostable Secondary Metabolite from Bacillus thuringiensis with Insecticidal Activity against a Wide Range of Insects

Liu

Ruan

Peng

et al. 2014

Toxins

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“…Cyclic adenosine monophosphate (cAMP) is an important compound which exists in many living cells, and participates in the regulation of physiological actions, such as cell proliferation and differentiation, hormones synthesis and secretion, membrane protein activity, nervous activity, gene expression and so on 1 – 3 . Based on its functions, cAMP is used in pharmaceuticals and as feed additives.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptomic and proteomic analysis of Arthrobacter sp. CGMCC 3584 responding to dissolved oxygen for cAMP production

Niu

Wang

Zhuang

et al. 2018

Sci Rep

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Arthrobacter sp. CGMCC 3584 is able to produce high yields of extracellular cyclic adenosine monophosphate (cAMP), which plays a vital role in the field of treatment of disease and animal food, during aerobic fermentation. However, the molecular basis of cAMP production in Arthrobacter species is rarely explored. Here, for the first time, we report the comparative transcriptomic and proteomic study of Arthrobacter cells to elucidate the higher productivity of cAMP under high oxygen supply. We finally obtained 14.1% and 19.3% of the Arthrobacter genome genes which were up-regulated and down-regulated notably, respectively, with high oxygen supply, and identified 54 differently expressed proteins. Our results revealed that high oxygen supply had two major effects on metabolism: inhibition of glycolysis, pyruvate metabolism, nitrogen metabolism, and amino acid metabolism (histidine, branched-chain amino acids and glutamate metabolism); enhancement of the tricarboxylic acid cycle and purine metabolism. We also found that regulation of adenylate cyclase and phosphodiesterase was not significant under high oxygen supply, suggesting efficient cAMP export might be important in cAMP production. These findings may contribute to further understanding of capacities of Arthrobacter species and would be highly useful in genetic regulation for desirable production.Cyclic adenosine monophosphate (cAMP) is an important compound which exists in many living cells, and participates in the regulation of physiological actions, such as cell proliferation and differentiation, hormones synthesis and secretion, membrane protein activity, nervous activity, gene expression and so on 1-3 . Based on its functions, cAMP is used in pharmaceuticals and as feed additives. The industrial production of cAMP through microorganism attracts attention of researchers for low cost and environmental friendliness. The microbes, including Microbacterium, Arthrobacter, Brevibacterium liquefacien, Corynebacterium roseoparaffineus, Corynebacterium murisepticum, were reported to be able to produce cAMP 4,5 . Our previous studies have demonstrated that a strain, Arthrobacter sp. CGMCC 3584, was capable of producing high yields of cAMP in culture media, through optimization of culture conditions and metabolic regulation on the fermentation process [6][7][8] . Arthrobacter species are among the most common aerobic culturable bacteria and exist in the nature widely, mainly in soil and in some extreme environments 9,10 . Their remarkable tolerance to various stresses contributes to the application of Arthrobacter species in contaminant degradation in complex and volatile environments 11 . Most genome and transcriptome researches of this genus focus on the genetic basis of the biodegradation and survival capacities, and available microarray data are rarely thus far. In the present study, DNA microarray-based transcriptomic analysis and two-dimensional (2D) gel electrophoresis technology

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“…through ion channels (Hong and Peng 2003;Ando et al 1987). Due to its important functions, cAMP is widely used in animal pharmaceutical research (Hong and Peng 2003;Tsai et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Production of cyclic adenosine monophosphate by Arthrobacter sp. A302 using fed-batch fermentation with pH-shift control

Cao

Chen

Ren

et al. 2011

World J Microbiol Biotechnol

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The production of cyclic adenosine monophosphate (cAMP) by Arthrobacter sp. A302 was studied in a 5 L stirred tank fermentor under a range of pH values (6.5-8.0) and glucose feeding rates. In batch fermentation under a controlled pH, the optimum pH for cell growth was 7.5 with dry cell density (X) of 11.43 g L, and the optimum pH for cAMP accumulation was 7.0 with cAMP concentration of 7.41 g L. In order to achieve the high X and cAMP yield simultaneously, a pH-shift control strategy was proposed based on kinetic analysis of specific cell growth rate (μ) and specific cAMP formation rate (q ( s )). In this method, pH was controlled to 7.0 for the first 30 h of fermentation, and then subsequently shifted to 7.5 and maintained until the end of the process. Application of this approach significantly enhanced the cAMP concentration. Thereafter, cAMP production was further improved by combining the above-mentioned pH-control system and fed-batch process with glucose at a constant feeding rate of 1.0 g L(-1 )h(-1). Under optimum conditions, the final cAMP production was 10.87 g L, which is 110.0, 46.7, and 27.7% higher than that of the pH-uncontrolled, pH-controlled, and pH-shift controlled methods, respectively.

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Effect of thuringiensin on adenylate cyclase in rat cerebral cortex

Cited by 16 publications

References 29 publications

Thuringiensin: A Thermostable Secondary Metabolite from Bacillus thuringiensis with Insecticidal Activity against a Wide Range of Insects

Thuringiensin: A Thermostable Secondary Metabolite from Bacillus thuringiensis with Insecticidal Activity against a Wide Range of Insects

Comparative transcriptomic and proteomic analysis of Arthrobacter sp. CGMCC 3584 responding to dissolved oxygen for cAMP production

Production of cyclic adenosine monophosphate by Arthrobacter sp. A302 using fed-batch fermentation with pH-shift control

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