Effect of TiO2 Nanoparticles on Antioxidant Enzymes Activity and Biochemical Biomarkers in Pinto Bean (Phaseolus vulgaris L.)

Abstract

Tests were done on the effects of titanium dioxide spray on Pinto bean (Phaseolus vulgaris L. c.v 'c.o.s.16'). The study was conducted as a factorial experiment in a randomized complete block design with four replications for two years (2014 -2015). Treatments consisted of two factors; the first factor was stage of plant growth that spraying was applied (rapid vegetative growth, flowering and pod filling); and the second factor was that of different concentrations of titanium dioxide nanoparticles (TiO 2 ) that consisted of spray with water (control), nano titanium dioxide at concentrations of 0.01%, 0.02%, 0.03% and 0.05%. Activity of guaiacol peroxidase (GPX), activity of superoxide dismutase (SOD), activity of catalase (CAT), activity of peroxidase (POD), malonyldialdehyde (MDA) Content and 8-deoxy-2-hydroxyguanosine (8-OHDG) content were assayed. Results showed that effect of nano TiO 2 was significant on activity of superoxide dismutase (SOD), activity of catalase (CAT), activity of peroxidase (POD), malonyldialdehyde (MDA) Content and 8-deoxy-2-hydroxyguanosine (8-OHDG) content. Results of combined analysis of variance showed that the effect year significantly affected on SOD and 8-OH-2-DG (P ≤ 0.05). The effect of different amounts of titanium dioxide nanoparticles (TiO 2 ) significantly affected (P ≤ 0.05) on MDA and 8-OH-2-DG. The effects of different amounts of titanium dioxide nanoparticles and year were significant on SOD, POD, MDA and the amount of 8-deoxy-2-hydroxyguanosine in P ≤ 0.05. None of the physiological traits were affected by spraying of nano titanium dioxide. The effects of TiO 2 nanoparticles times of spraying and year were significant on SOD, CAT and 8-deoxy-2-hydroxyguanosine (P ≤ 0.05). Interaction effects of nano TiO 2 concentrations × nano TiO 2 spraying times did not have a significant impact on SOD, CAT, POD, GPX, MDA and 8-OH-2-DG. Although, all trait were affected by interaction effects of year × nano TiO 2 concentrations × nano TiO 2 spraying times with the exception of GPX (P ≤ 0.05).

Amendment of Agricultural Soil with Metal Nanoparticles: Effects on Soil Enzyme Activity and Microbial Community Composition

“…Ebrahimi et al showed that nTiO 2 exposure could increase the activity of antioxidant enzymes and inhibit ROS in pinto bean leaves . Servin et al showed that inorganic soil exposed to 250–750 mg/kg nTiO 2 for 120 days can increase catalase activity, while inhibiting ascorbate peroxidase activity in cucumber plant leaves .…”

“…Ebrahimi et al showed that nTiO 2 exposure could increase the activity of antioxidant enzymes and inhibit ROS in pinto bean leaves. 57 Servin et al showed that inorganic soil exposed to 250−750 mg/kg nTiO 2 for 120 days can increase catalase activity, while inhibiting ascorbate peroxidase activity in cucumber plant leaves. 58 Laware and Raskar found that amylase, protease, catalase, and peroxidase activities increased when exposed to low concentrations of nTiO 2 (10−30 μg/mL) for 7 days but decreased in the presence of higher concentrations of nTiO 2 (50 μg/mL).…”

Seed germination with titanium dioxide nanoparticles enhances water supply, reserve mobilization, oxidative stress and antioxidant enzyme activities in pea

“…NP treatments triggered the accumulation of reactive oxygen species, increased production of stress enzymes, lipid peroxidation that mediated oxidative stress in lentils ( Khan et al, 2019 ). Ebrahimi et al (2016) showed the installation of oxidative stress in Phaseolus vulgaris seedlings and disorder in antioxidant systems after treatment with TiO 2 NPs. In the same way, we showed that TiO 2 induced the antioxidant enzyme activities guaiacol peroxidase and catalase ( Fig.…”

​Effects of Foliar Application of Nano-Se On Photosynthetic Characteristics and Se Accumulation in Paeonia Ostii

“…It is an important parameter to re ect the potential antioxidant capacity of organisms, and an important index of plant aging and resistance physiology. Thus, to assess the extent of damage to the plasma membrane system peroxidation and resistance of plants, the degree of membrane lipid peroxidation can be determined by measuring MDA content (La et al 2011;Ebrahimi et al 2016). In this study, compared with those in CK, Se treatment signi cantly increased SOD and POD activity of Feng Dan, and signi cantly reduced MDA content, maybe owing to Se directly participating in the enzymatic or non-enzymatic antioxidant process of organisms as a component of glutathione peroxidase (GSH-Px) system.…”