Background: Tobacco consumption in any form is a well known health hazard and a major cause of mortality. This may affect male reproductive function and conveniently evaluated by semen analysis. Thus semen parameters of the subjects with habits of consumption compared within cases and control groups. Methods: Subjects referred for semen analysis & volunteers were studied. Detailed history of personal, occupational and tobacco consumption was obtained. Semen analysis performed as per the WHO guideline 2010, 5th edition. We included tobacco smokers, chewers and nonaddicted subjects. Tobacco consumers further stratified according to type, amount and duration of consumption and various semen parameters from these groups compared by using ANOVA and unpaired-t test. Result: Out of 157 subjects, 25 as control, 32-tobacco smokers, 87-tobacco chewers, 13 with both habits, further stratified according to amount and duration of consumption. There is significant reduced semen parameters of tobacco consumers obtained in relation to its amount of consumption were-sperm concentration, motility and morphology, while not affected with duration of consumption. Oligozoospermia was higher in tobacco consumers. Conclusion: Results of study found that tobacco consumption in men is associated with reduced sperm quality (including count, motility & morphology). This concludes tobacco consumption is one of the risk factor in male infertility and subfertility.