Effect of toluene extraction on Biomer™ surface: II. An atomic force microscopy study

Nurdin, Nathalie; Descouts, P.

doi:10.1163/156856295x00436

Cited by 6 publications

(1 citation statement)

References 21 publications

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“…The height differences between the lower and higher spots were around 3 nm. Nurdin et al (47) found for a similar polyurethane surface, after drying it in the vacuum, a peak-to-valley distance of about 15 nm. The plain and homogeneous surface morphology of the investigated polyurethane is well suited for the planned application.…”

Section: Resultsmentioning

confidence: 95%

Immobilization of Biomolecules on Polyurethane Membrane Surfaces

et al. 2001

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Lipophilic polymer membranes incorporating binding sites are widely used in various potentiometric, amperometric, and optical sensors. Here, we report on the biofunctional modification of the surface of a Ca(2+)-selective membrane. A photoactivatable biotin derivative was synthesized and covalently immobilized on a soft polyurethane membrane. The modified polymer was characterized by X-ray photoelectron spectroscopy (XPS) as well as by potentiometric measurements. The selective binding of streptavidin by the photo-cross-linked biotin derivative was demonstrated. The surface coverage obtained with different experimental protocols was analyzed by autoradiography using [(35)S]-streptavidin. The new approach may significantly extend the scope of applicability of potentiometric sensors.

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Section: Resultsmentioning

confidence: 95%