Effect of traditional Uyghur medicine abnormal Savda Munziq extract on rabbit platelet aggregation in vitro and rat arteriovenous shunt thrombosis in vivo

Umar, Asad; Yimin, Wuliya; Tohti, Ibadet; Upur, Halmurat; Berké, Bénédicte; Moore, Nicholas

doi:10.1016/j.jep.2014.11.006

Cited by 13 publications

(6 citation statements)

References 53 publications

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“…Each group was administered once a day for 5 consecutive days. The bleeding time was evaluated via the method devised by Seiji Kaku et al (2013) with some modifications [ 32 , 33 , 34 ] Thirty minutes after the last administration, the caudal parts were cut perpendicularly at a distance of 5 mm from the caudal end. We started counting every 30 s when the blood spilled out.…”

Section: Methodsmentioning

confidence: 99%

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Anticoagulant Activities of Indobufen, an Antiplatelet Drug

Liu¹,

Xia

et al. 2018

Molecules

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Indobufen is a new generation of anti-platelet aggregation drug, but studies were not sufficient on its anticoagulant effects. In the present study, the anticoagulant activity of indobufen was determined by monitoring the activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) in rabbit plasma. We evaluated the anticoagulant mechanisms on the content of the platelet factor 3,4 (PF3,4), and the coagulation factor 1, 2, 5, 8, 10 (FI, II, V, VIII, X) in rabbits, as well as the in vivo bleeding time and clotting time in mice. The pharmacodynamic differences between indobufen and warfarin sodium, rivaroxaban, and dabigatran were further studied on thrombus formation and the content of FII and FX in rats. Animal experiments showed that intragastric-administrated indobufen can significantly reduce the APTT, PT, TT, PF3, FI, II, V, VIII, and X plasma contents. Its inhibitory effect on plasma FII was better than thrombin inhibitor dabigatran with effect on FX better than FXa inhibitor rivaroxaban. These results suggest that indobufen has some anticoagulant effects as strong as some conventional anticoagulants. The mechanism may be related to both exogenous and endogenous coagulation system.

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Section: Methodsmentioning

confidence: 99%

“…The total weight minus the thread weight is the dry thrombus weight after the thread was dried at 70 °C to a constant weight. We calculated the inhibition rate of both wet and dry thrombus weight [ 34 ]

…”

Section: Methodsmentioning

confidence: 99%

Anticoagulant Activities of Indobufen, an Antiplatelet Drug

Liu¹,

Xia

et al. 2018

Molecules

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“…The procedure for inducing arteriovenous shunt thrombosis was performed as described previously [26,27]. D609 and a control solvent (1 mL/min) were injected through the jugular vein.…”

Section: Arteriovenous Shunt Thrombosismentioning

confidence: 99%

The role of Sphingomyelin synthase 2 (SMS2) in platelet activation and its clinical significance

et al. 2021

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Background Sphingomyelin (SM) is an essential component of biological lipid rafts, and it plays an indispensable role in maintaining plasma membrane stability and in mediating signal transduction. The ultimate biosynthesis of SM is catalyzed by two sphingomyelin synthases (SMSs) namely SMS1 and SMS2, which are selectively distributed in the trans-Golgi apparatus and the plasma membrane. It has been demonstrated that SMS2 acts as an irreplaceable molecule in the regulation of transmembrane signaling, and loss of SMS2 has been reported to worsen atherosclerosis and liver steatosis. However, the function of SMS2 in platelet activation and its association with the pathological process of thrombosis in acute coronary syndrome (ACS) and portal hypertension (PH) remain unclear. Methods In this study, we tested the role of SMS2 in platelet activation and thrombosis using SMS2 knockout (SMS2 –/–) mice and SMS2-specific inhibitor, D609. Furthermore, we detected SMS2 expression in patients with ACS and PH. Results SMS2 –/– platelets showed significant reduction in platelet aggregation, spreading, clot retraction and in vivo thrombosis. Similar inhibitory effects on platelet activation were detected in D609-treated wild-type platelets. PLCγ/PI3K/Akt signaling pathway was inhibited in SMS2 –/– platelets and D609-treated wild-type platelets. In addition, we discovered that platelet SMS2 expression was remarkably increased in patients with ACS and PH, compared with healthy subjects. Conclusions Our study indicates that SMS2 acts as a positive regulator of platelet activation and thrombosis, and provides a theoretical basis for the potential use of D609 in anti-thrombosis treatment.

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“…As described in Table 1 , MNZQ comprises 13 species of medicinal plants, including seeds of Peganum harmala , Cichorium intybus , Dracocephalum moldavica , Ocimum basilicum , Althaea rosea , and Nigella glandulifera ; fruits of Pimpinella anisum ; roots of Apium graveolens , Glycyrrhiza uralensis and Cichorium intybus ; Cortex of Foeniculum vulgare ; and herbs of Matricaria chamomilla and Cymbopogon caesius . According to TUM, excess or deficiency in one or more of the four body fluids, namely, Khan (blood), phlegm, Sapra (yellow bile), and Savda (black bile), can influence human metabolism and cause diseases [ 8 , 9 ]. As a maturative agent of the four abnormal body fluids, MNZQ can help these fluids recover from imbalances and treat diseases.…”

Section: Introductionmentioning

confidence: 99%

In in vivo evaluation of the anti-inflammatory and analgesic activities of compound Muniziqi granule in experimental animal models

Cheng

Liu

et al. 2015

BMC Complement Altern Med

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Background: Compound Muniziqi granule (MNZQ), a traditional Uighur medicinal preparation, comprises 13 species of medicinal plants. MNZQ is traditionally used for regulating body immunity, modulating inflammation and pain, detoxification, and inhibiting tumor growth. This study aims to scientifically evaluate the anti-inflammatory and analgesic activities of MNZQ, support its clinical use and further research with scientific evidence. Methods: The analgesic activity of MNZQ was evaluated using hot plate test and acetic acid-induced abdominal writhing test. Acute inflammation was evaluated using xylene-induced ear edema and carrageenan-induced paw edema models, while chronic inflammation was evaluated using cotton pellet-induced granuloma model. Results: MNZQ exerted analgesic activities with a significant dose-dependent increase in latency in the hot plate test. The percentage inhibition suggested that MNZQ exhibited analgesic activities in the central nervous system. Meanwhile, MNZQ at 0.8, 2.4, and 7.2 g/kg strongly inhibited the acetic acid-induced writhing response by 25.22 % (p < 0.01), 44.60 % (p < 0.001), and 49.41 % (p < 0.001), respectively. MNZQ also exerted analgesic activities in the peripheral nervous system. Moreover, MNZQ was demonstrated a significant anti-inflammatory effect against xylene-induced edema in a dose-dependent manner. The percentage inhibition was 22.24 % (p < 0.01) at the highest dosage of 7.2 g/kg. MNZQ at 1.62 and 4.86 g/kg significantly reduced carrageenan-induced rat hind paw edema by 82.43 % and 84.32 % (p < 0.001), respectively, 1 h after injecting carrageenan, and the inhibitory effect lasted for 5 h. MNZQ also exerted a significant anti-inflammatory effect against cotton pellet-induced granuloma formation. MNZQ at 1.62 and 4.86 g/kg could inhibit granuloma formation by 17.07 % and 17.60 %, respectively, whereas the percentage inhibition of diclofenac was 33.12 %. Conclusions: The results obtained suggest that MNZQ possesses potential anti-inflammatory and analgesic activities. This study provides a scientific basis for the use of MNZQ in alleviating pain and treating inflammatory disorders.

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Effect of traditional Uyghur medicine abnormal Savda Munziq extract on rabbit platelet aggregation in vitro and rat arteriovenous shunt thrombosis in vivo

Cited by 13 publications

References 53 publications

Anticoagulant Activities of Indobufen, an Antiplatelet Drug

Anticoagulant Activities of Indobufen, an Antiplatelet Drug

The role of Sphingomyelin synthase 2 (SMS2) in platelet activation and its clinical significance

In in vivo evaluation of the anti-inflammatory and analgesic activities of compound Muniziqi granule in experimental animal models

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