Effect of Treatment of Latent Tuberculosis Infection on the T Cell Response to<i>Mycobacterium tuberculosis</i>Antigens

Wilkinson, Katalin A.; Kon, Onn Min; Newton, Sandra M.; Meintjes, Graeme; Davidson, Robert N.; Pasvol, Geoffrey; Wilkinson, Robert J.

doi:10.1086/499311

Cited by 109 publications

(101 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This highly encouraging finding therefore suggests that the IFNGRA could better identify HIV-infected persons at risk of tuberculosis. However, the median CD4 ϩ cell count of our patients was relatively high at 392/mm 3 and there was a trend toward decreased positivity for QFT-G in those with counts less than 250/mm 3 ( Figure 4). It is also arguable that in an area where the incidence of tuberculosis is 1,612 per 100,000, a high proportion of positive IFNGRAs was expected and might be expected to be higher still in the HIV-infected group, the group most susceptible to tuberculosis.…”

Section: Discussionmentioning

confidence: 72%

“…Detection of latent or recent infection, which represents the reservoir of future tuberculosis cases, is an opportunity for intensified control (3). In HIV-infected people with latent tuberculosis infection (LTBI), isoniazid preventive therapy (IPT) is an underutilized public health strategy (4).…”

Section: What This Study Adds To the Fieldmentioning

confidence: 99%

“…Scoring was carried out by a third investigator who had no role in clinical recruitment or in performing the laboratory assays. In HIV-infected persons, the CD4 ϩ cell count was simultaneously determined: persons qualifying for antiretroviral therapy (CD4 ϩ cell count less than 200/mm 3 or World Health Organization clinical stage 4) were referred to the antiretroviral clinic.…”

Section: Laboratory Assaysmentioning

confidence: 99%

See 2 more Smart Citations

Effect of HIV-1 Infection on T-Cell–based and Skin Test Detection of Tuberculosis Infection

Rangaka¹,

Wilkinson²,

Seldon³

et al. 2007

Am J Respir Crit Care Med

186

152

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 72%

Section: What This Study Adds To the Fieldmentioning

confidence: 99%

Section: Laboratory Assaysmentioning

confidence: 99%

See 1 more Smart Citation

Effect of HIV-1 Infection on T-Cell–based and Skin Test Detection of Tuberculosis Infection

Rangaka¹,

Wilkinson²,

Seldon³

et al. 2007

Am J Respir Crit Care Med

186

152

View full text Add to dashboard Cite

“…The presence of specific IFN-␥ spot-forming cells (SFCs) is indicative of an M. tuberculosis infection and seems to correlate better than the TST with the level of exposure to M. tuberculosis (3). In this context, the IFN-␥ ELISpot assay is considered to be a major advance in TB diagnostics.The IFN-␥ ELISpot assay has also been tested as a means to monitor the response to TB therapy, where efficacy is classically evaluated by measuring rates of relapse 6 to 12 months after treatment completion (11,43). On the basis of the hypothesis that the IFN-␥ ELISpot assay could function as a bacterial load sensor, a comparatively smaller amount of SFCs in response to ESAT-6 and/or CFP-10 over time would be indicative of a good response to treatment, leading to a lower probability of relapse.…”

mentioning

confidence: 99%

“…The IFN-␥ ELISpot assay has also been tested as a means to monitor the response to TB therapy, where efficacy is classically evaluated by measuring rates of relapse 6 to 12 months after treatment completion (11,43). On the basis of the hypothesis that the IFN-␥ ELISpot assay could function as a bacterial load sensor, a comparatively smaller amount of SFCs in response to ESAT-6 and/or CFP-10 over time would be indicative of a good response to treatment, leading to a lower probability of relapse.…”

mentioning

confidence: 99%

T Cell Monitoring of Chemotherapy in Experimental Rat Tuberculosis

Foo

Tay

Siew

et al. 2011

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Mycobacterium tuberculosis is the causative agent of a pulmonary epidemic that is estimated to infect one-third of the world's population and that has an increased incidence of multidrug resistance. The evaluation of new chemical entities against M. tuberculosis is hampered by the lack of biological tools to help predict efficacy, from early drug development to clinical trials. As the rat is the animal species of choice in the pharmaceutical industry, we have developed a rat model of acute and chronic phases of M. tuberculosis infection for drug efficacy testing. In this model, we have evaluated the impact of tuberculosis drugs on T cell response using the enzyme-linked immunospot assay methodology. Infected rats treated with isoniazid (INH) or rifampin (RIF) responded to therapy, the potency of which was comparable to that seen in the mouse. Peripheral blood mononuclear cells from infected rats produced gamma interferon (IFN-␥) in response to RD-1 antigens, such as the 6-kDa early secretory antigen target (ESAT-6) and the 10-kDa culture filtrate protein (CFP-10). A decrease in IFN-␥ spot-forming cells (SFCs) was consistently observed in response to drug treatment. In both the acute-and chronic-phase models, the T cell response was more sensitive to ESAT-6 than to CFP-10. The SFC count in response to ESAT-6 appears to be an indicator of bacterial killing in the rat. Collectively, our data suggest that the ESAT-6 response could be used as a potential surrogate of drug efficacy in the rat and that such a readout could help shorten drug testing during preclinical development.Mycobacterium tuberculosis infection is among the world's leading infectious diseases, causing about 2 million deaths annually. The emergence of multidrug-resistant M. tuberculosis strains along with the increase of HIV coinfected cases worsens the situation (42). In countries with a high incidence of tuberculosis (TB), TB control programs rely on a diagnostic methods and drugs that have been developed decades ago and that are inadequate to effectively control the epidemic. The urgent need to develop new diagnostic tools as well as new therapeutic interventions is hampered by long clinical trials, where markers of infection and drug response are lacking (38).For decades, the tuberculin skin test (TST) has been used to diagnose TB (18). The TST measures cell-mediated immunity in the form of a delayed-type hypersensitivity response to the purified protein derivative (PPD), a crude mixture of antigens shared among M. tuberculosis, Mycobacterium bovis BCG, and several nontuberculous mycobacteria (NTM). As a result, the TST has lower specificity in populations with high BCG coverage and NTM exposure and shows poor sensitivity in immunocompromised individuals (30). The gamma interferon (IFN-␥) enzyme-linked immunospot (ELISpot) assay has emerged as an alternative to the TST. The assay consists of in vitro stimulation of peripheral blood mononuclear cells (PBMCs) using RD-1 antigens, the 6-kDa early secretory antigen target (ESAT-6) and the 10-kDa cul...

show abstract

Effectiveness of the combination of a whole‐blood interferon‐gamma assay and the tuberculin skin test in detecting latent tuberculosis infection in rheumatoid arthritis patients receiving adalimumab therapy

Chen¹,

Shen²,

Hsieh³

et al. 2008

Arthritis & Rheumatism

View full text Add to dashboard Cite

Objective. To investigate QuantiFERON-tuberculosis Gold (QFT-G) assay and tuberculin skin test (TST) for latent tuberculosis (TB) infection (LTBI) in patients with rheumatoid arthritis (RA) treated with adalimumab. Methods. We prospectively followed up 43 RA patients who received adalimumab therapy and underwent serial TSTs and QFT-G assays. TST was performed using Mantoux method and QFT-G assay was examined by measuring interferon-␥ levels in whole blood samples that were incubated with early secretary antigenic target-6 and culture filtrate protein 10.

show abstract

Effect of Treatment of Latent Tuberculosis Infection on the T Cell Response toMycobacterium tuberculosisAntigens

Cited by 109 publications

References 24 publications

Effect of HIV-1 Infection on T-Cell–based and Skin Test Detection of Tuberculosis Infection

Effect of HIV-1 Infection on T-Cell–based and Skin Test Detection of Tuberculosis Infection

T Cell Monitoring of Chemotherapy in Experimental Rat Tuberculosis

Effectiveness of the combination of a whole‐blood interferon‐gamma assay and the tuberculin skin test in detecting latent tuberculosis infection in rheumatoid arthritis patients receiving adalimumab therapy

Contact Info

Product

Resources

About