Effect of tuftsin on the functional activity and intracellular pH of murine peritoneal macrophages

Abstract

267 scent HDL. The content of total serum Ch dropped by 38% (p<0.01) and by 41% (p<0.01) after the administration of compound II and compound I, respectively. However, the level of c~-Cli did not drop, and in the case of compound II it even rose 22%, i.e., the drop of total Ch occurred due to LDL and VLDL Ch. Both test compounds increased the content of Ch in the liver, whereas accumulation of TG was observed only in the case of compound I (Table 2).Thus, the effect of compounds I and II on serum LP and on the content of Ch in blood serum and in the liver surpasses that of estradiol, which does not normalize the LP spectrum in ovariectomized animals and even alters it adversely in experimental animals. Compound II possesses a less pronounced hypolipidemic activity in comparison with compound I; however, its effect on the serum LP spectrum is more favorable.

Effect of a peptide extract of fetal brain tissue on the intracellular pH of mouse peritoneal phagocytes

“…Effects of the peptide extract in dilutions 1:104 (0.2 gg/ml) and 1:10'-(20 gg/ml) were investigated. These dilutions were selected because, as had been previously shown, well-expressed cell responses to the regulatory tetrapeptide taftsin [7,11] and the drug tactivin, representing a complex of thymic regulatory peptides [1], are observed with such concentrations. Figure 1 shows that the efficacy of the drug differed for the two cell types examined.…”

“…Previously described methods [6,7] with some modifications were used. Experiments were carried out on peritoneal neutrophils and macrophages of outbred male albino mice.…”

“…Shifts in the intracellular pH values registered during such events are commonly 0.1 to 0.2 U. Of special interest are the cells helping provide for the immune homeostasis of the organism, including phagocytic cells (neutrophils and macrophages), the specific aspects of whose functional activity are realized with the active participation of H + ions [3,7,8,14,15].…”

Application of Phase Images for Estimation of Peritoneal Macrophages State

“…The percentage of cells with damaged membranes (dead cells) was estimated using ethidium bromide in final concentration 5 µg/ml (Serva) (Dankberg & Persidsky, 1976;Turovetskii et al, 1994). The cell damage was calculated as a ratio of the number of damaged cells to total cell number.…”