Effect of two lytic bacteriophages against multidrug-resistant and biofilm-forming <i>Salmonella</i> Gallinarum from poultry

Rizzo, Natalie Nadin; Pottker, Emanuele Serro; Webber, Bruna; Borges, Karen Apellanis; Duarte, Sabrina Castilho; Levandowski, Rafael; Ruschel, L.; Rodrigues, Laura Beatriz

doi:10.1080/00071668.2020.1805724

Cited by 8 publications

(12 citation statements)

References 42 publications

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“…As already known in the literature, phages are species-specific (Reardon, 2017b), but we stress that these same phages have demonstrated decent antimicrobial performance in previous studies against other serovars of Salmonella spp., such as S. Brandemburg, S. Anatum, S. Tennessee, S. Agona, S. Bredney, S. Schwarzemgrund, S. Infantil, S. Rissen, S. Lexington, S. Panama, S. Enteritidis, S. Typhimurium and S. Gallinarum (Pottker et al, 2020;Rizzo et al, 2020). These results indicated the phage action in a wide variety of serovars, possibly being specific for the Salmonella genus.…”

Section: Resultsmentioning

confidence: 75%

“… a Multiple antibiotic resistance index. MARI ≥ 0.2 is an indicator related to the phenomenon of multiple resistance (Krumperman, 1983; Rizzo et al, 2020). b Lysis results were recorded as decreasing lysis effect as follows: +++: confluent lysis; ++: semi-confluent lysis; +: individual plaques; S: shadow lysis; –: no plaque. …”

Section: Resultsmentioning

confidence: 99%

“… a Multiple antibiotic resistance index. MARI ≥ 0.2 is an indicator related to the phenomenon of multiple resistance (Krumperman, 1983; Rizzo et al, 2020). …”

Section: Resultsmentioning

confidence: 99%

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Surface conditioning with bacteriophages reduces biofilm formation of Salmonella Heidelberg

Webber

Pottker

Rizzo

et al. 2022

Food sci. technol. int.

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Salmonella remains one of the most common foodborne pathogens worldwide, and its resistance to antimicrobials has increased considerably over the years. In this context, was evaluated the action of three bacteriophages isolated or combined in inhibiting the adhesion and removal of Salmonella Heidelberg biofilm on a polystyrene surface. The bacteriophages UPF_BP1, UPF_BP2, UPF_BP3 and a pool of them were used for biocontrol of Salmonella Heidelberg biofilms on polystyrene surface in the action times of 3, 6 and 9 h. Individual and combined phages exhibited reductions in Salmonella Heidelberg adhesion of up to 83.4% and up to 64.0% in removal of preformed biofilm. The use of synergistic combinations between the phages is the most indicated option due to its potential to reduce biofilms. The use of the bacteriophage pool in surface conditioning is an alternative in the control of Salmonella Heidelberg biofilms.

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Section: Resultsmentioning

confidence: 75%

Section: Resultsmentioning

confidence: 99%

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Surface conditioning with bacteriophages reduces biofilm formation of Salmonella Heidelberg

Webber

Pottker

Rizzo

et al. 2022

Food sci. technol. int.

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“…On the other hand, catheter-associated biofilm clearance may involve with an alternative approach where phage is used as a gel-like coating on the catheter to reduce the bacterial adhesion to the surfaces ( Curtin and Donlan, 2006 ; Maszewska et al, 2018 ). Furthermore, since the excessive misuse of traditional antibiotics has led to a striking rise in cases related to drug-resistant bacterial infections ( World Health Organization [WHO], 2020 ), phage therapy has recently shifted the focus on eradicating biofilms produced by multi-drug resistant (MDR) bacteria such as MDR Enterobacter cloacae ( E. cloacae) ( Jamal et al, 2019 ), MDR S. aureus ( Cha et al, 2019 ), MDR P. aeruginosa ( Yuan et al, 2019 ; Adnan et al, 2020 ), and MDR Salmonella gallinarum ( S. gallinarum) , ( Rizzo et al, 2020 ) to name a few. The diagnostic assays for measuring anti-biofilm activity of phages include fluorescence microscopy ( Shahed-Al-Mahmud et al, 2021 ), LIVE/DEAD BacLight Bacterial Viability Kit ( Latka and Drulis-Kawa, 2020 ), etc.…”

Section: How Phages Combat Biofilmmentioning

confidence: 99%

Bacteriophage-Mediated Control of Biofilm: A Promising New Dawn for the Future

Chang

Guo

et al. 2022

Front. Microbiol.

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Biofilms are complex microbial microcolonies consisting of planktonic and dormant bacteria bound to a surface. The bacterial cells within the biofilm are embedded within the extracellular polymeric substance (EPS) consisting mainly of exopolysaccharides, secreted proteins, lipids, and extracellular DNA. This structural matrix poses a major challenge against common treatment options due to its extensive antibiotic-resistant properties. Because biofilms are so recalcitrant to antibiotics, they pose a unique challenge to patients in a nosocomial setting, mainly linked to lower respiratory, urinary tract, and surgical wound infections as well as the medical devices used during treatment. Another unique property of biofilm is its ability to adhere to both biological and man-made surfaces, allowing growth on human tissues and organs, hospital tools, and medical devices, etc. Based on prior understanding of bacteriophage structure, mechanisms, and its effects on bacteria eradication, leading research has been conducted on the effects of phages and its individual proteins on biofilm and its role in overall biofilm removal while also revealing the obstacles this form of treatment currently have. The expansion in the phage host-species range is one that urges for improvement and is the focus for future studies. This review aims to demonstrate the advantages and challenges of bacteriophage and its components on biofilm removal, as well as potential usage of phage cocktail, combination therapy, and genetically modified phages in a clinical setting.

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“…Noteworthy, the association of some resistant S. Gallinarum isolates with the historical indiscriminate use of antibiotics has already been demonstrated [75,92]. Antimicrobial therapy is also described to treat PD and FT, especially in small-scale commercial layer flocks [95].…”

Section: Salmonella Gallinarummentioning

confidence: 99%

Emergence, Dissemination and Antimicrobial Resistance of the Main Poultry-Associated Salmonella Serovars in Brazil

Kipper

Mascitti

Carli

et al. 2022

Veterinary Sciences

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Salmonella infects poultry, and it is also a human foodborne pathogen. This bacterial genus is classified into several serovars/lineages, some of them showing high antimicrobial resistance (AMR). The ease of Salmonella transmission in farms, slaughterhouses, and eggs industries has made controlling it a real challenge in the poultry-production chains. This review describes the emergence, dissemination, and AMR of the main Salmonella serovars and lineages detected in Brazilian poultry. It is reported that few serovars emerged and have been more widely disseminated in breeders, broilers, and layers in the last 70 years. Salmonella Gallinarum was the first to spread on the farms, remaining as a concerning poultry pathogen. Salmonella Typhimurium and Enteritidis were also largely detected in poultry and foods (eggs, chicken, turkey), being associated with several human foodborne outbreaks. Salmonella Heidelberg and Minnesota have been more widely spread in recent years, resulting in frequent chicken/turkey meat contamination. A few more serovars (Infantis, Newport, Hadar, Senftenberg, Schwarzengrund, and Mbandaka, among others) were also detected, but less frequently and usually in specific poultry-production regions. AMR has been identified in most isolates, highlighting multi-drug resistance in specific poultry lineages from the serovars Typhimurium, Heidelberg, and Minnesota. Epidemiological studies are necessary to trace and control this pathogen in Brazilian commercial poultry production chains.

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Effect of two lytic bacteriophages against multidrug-resistant and biofilm-forming Salmonella Gallinarum from poultry

Cited by 8 publications

References 42 publications

Surface conditioning with bacteriophages reduces biofilm formation of Salmonella Heidelberg

Surface conditioning with bacteriophages reduces biofilm formation of Salmonella Heidelberg

Bacteriophage-Mediated Control of Biofilm: A Promising New Dawn for the Future

Emergence, Dissemination and Antimicrobial Resistance of the Main Poultry-Associated Salmonella Serovars in Brazil

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