Surface conditioning with bacteriophages reduces biofilm formation of <i>Salmonella</i> Heidelberg

Webber, Bruna; Pottker, Emanuele Serro; Rizzo, Natalie Nadin; Núncio, Adriana Sp; Peixoto, Caroline S; Mistura, Enzo; Santos, Luciana Ruschel dos; Rodrigues, Laura Beatriz; Nascimento, Vladimir Pinheiro do

doi:10.1177/10820132221074783

Cited by 5 publications

(3 citation statements)

References 39 publications

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“…Our study showed better results for the inhibition of Salmonella adhesion than for the removal of the preformed Salmonella biofilm. Similar results were found by Webber et al (2022) that demonstrated the effect of bacteriophages against S. Heidelberg biofilms. For studying preformed biofilms, the biofilms were allowed to form for 48 h, and were considered mature structures that hinder the action of the phage cocktail (Pires et al, 2017).…”

Section: Resultssupporting

confidence: 87%

Bacteriophages as an alternative for biological control of biofilm-forming Salmonella enterica

Pottker

Rodrigues

Borges

et al. 2022

Food sci. technol. int.

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Salmonellosis is one of the most common foodborne diseases worldwide. Surface adherence and biofilm formation are among the main strategies evolved by Salmonella to survive under harsh conditions and are risk factors for its spread through the food chain. Owing to the increase in antimicrobial resistance, there is a growing need to develop other methods to control foodborne pathogens, and bacteriophages have been suggested as a potential alternative for this purpose. The aim of this study was to evaluate bacteriophages as a biological control of Salmonella enterica serotypes to inhibit and remove bacterial biofilms. A total of 12 S. enterica isolates were selected for this study, all of which were biofilm producers. Seven bacteriophages were tested, individually and in a cocktail, for their host range and efficiency of plating (EOP). The phage cocktail was evaluated for its antibiofilm effect against the Salmonella biofilms. Phages UPF_BP1, UPF_BP2, UPF_BP3, UPF_BP6, and 10:2 possessed a broad lytic spectrum and could infect all S. enterica strains. Phages 10:2, UPF_BP6, and UPF_BP3 had high EOP in 10, 9, and 9 out of the 12 S. enterica strains, respectively. The cocktail was able to infect all S. enterica strains and had a high EOP in 10 out of 12 S. enterica isolates, presenting a broader host range than any of the tested single phages. A wide variation of inhibition among strains was observed, ranging from 14.72% to 88.53%. Multidrug-resistant and strong biofilm producer strains showed high biofilm inhibition levels by phage cocktail. Our findings demonstrate the ability of the cocktail to prevent biofilm formation and remove formed biofilms of Salmonella. These results indicate that the phage cocktail is a promising candidate to be used as an alternative for the control of Salmonella biofilms through surface conditioning.

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Section: Resultssupporting

confidence: 87%

Bacteriophages as an alternative for biological control of biofilm-forming Salmonella enterica

Pottker

Rodrigues

Borges

et al. 2022

Food sci. technol. int.

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“…Refer to Webber et al's methodology with minor modifications (48). Salmonella solution (100 μL, OD600 = 0.2) was added to 96-well plates and incubated at 37 °C for 72 h to form mature biofilms.…”

Section: Evaluation Of the Inhibitory Effect And Removal Of Salmonell...mentioning

confidence: 99%

Application of a novel phage W5 for biological control 1 of Salmonella Typhimurium in different food matrices

Chong,

Yang,

Fan

et al. 2024

Preprint

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Background: Salmonella, as one of the four major foodborne pathogens, poses a serious challenge to global public health. The aim of this paper is to apply phage eradication for biocontrol of salmonellosis in different food matrices. Results: This study isolated a novel lytic phage, designated as W5, isolated from livestock and poultry slaughterhouse effluent. W5 displayed effective lytic activity against prevalent Salmonella serotypes, including Salmonella Typhimurium, Salmonella Choleraesuis, and Salmonella Typhi. Morphological observations indicate that phage W5 belongs to the family Myoviridae and the order Caudoviridae, the whole genome sequencing and phylogenetic analysis also confirmed W5 as a novel species within the Cornellvirus genus of the Guernseyvirinae subfamily. The biological characterization revealed that phage W5 had an optimal infection complex of 1, with a brief incubation period of 22 min followed by a logarithmic phase extending from 22 min to 70 min to peak at a maximum of 3.67 × 1011 PFU/mL and achieving a plateau period after 70 min. Moreover, W5 exhibits robust thermal and acid–base stability, optimally active at 50 °C and stable across a pH range of 3 to 13. Neither SM buffer nor glycerol impacted the activity of W5, with 98% of the phage adsorbing to the host bacteria within 13 min of post-mixing. Further tests demonstrated that W5 can inhibit Salmonella in milk, pork, and eggs, effectively prevent biofilm formation, and remove biofilm. These results suggest that phage W5 is abundantly available as a potential antimicrobial agent for Salmonella control in the food industry, owing to its safety and effectiveness. Conclusions: Biological characterization, genome-wide analysis, and application of phage W5 showed that this phage is a promising candidate for use in phage mixtures or other phage-based preparations.

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“…Phages are the most abundant organisms in the biosphere, and they have been used in the health fields since the 1920s when they were first discovered by the French-Canadian microbiologist Félix d'Hérelle [ 68 , 69 ]. The application of bacteriophage has been considered a promising approach for controlling the biofilm formation of foodborne pathogens on the food industry surfaces [ 70 , 71 ]. Because phages can only infect bacteria, they are generally recognized as low toxic to human and eukaryote cells [ 72 , 73 ].…”

Section: Introductionmentioning

confidence: 99%

Controlling of foodborne pathogen biofilms on stainless steel by bacteriophages: A systematic review and meta-analysis

Azari,

Yousefi,

Fallah

et al. 2024

Biofilm

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Surface conditioning with bacteriophages reduces biofilm formation of Salmonella Heidelberg

Cited by 5 publications

References 39 publications

Bacteriophages as an alternative for biological control of biofilm-forming Salmonella enterica

Bacteriophages as an alternative for biological control of biofilm-forming Salmonella enterica

Application of a novel phage W5 for biological control 1 of Salmonella Typhimurium in different food matrices

Controlling of foodborne pathogen biofilms on stainless steel by bacteriophages: A systematic review and meta-analysis

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