Effect of Ultra-violet Light on Tobacco Mosaic Virus containing 5-Fluorouracil

Becarevic, A.; Djordjević, Božidar; Sutic, D.

doi:10.1038/198612a0

Cited by 21 publications

(5 citation statements)

References 10 publications

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“…The incorporation of the pyrimidine analog, 5fluorouracil, into the nucleic acid of tobacco mosaic virus sensitizes the intact virus (Becarevic et al, 1963; Lozeron and Gordon, 1964) and the infectious nucleic acid to ultraviolet light (Lozeron and Gordon, 1964). A study of the photochemistry of 5-fluorouracil has been initiated with the hope that the identification of some of the main photoproducts might be helpful in gaining insight into the molecular mechanism of ultraviolet sensitization of the 5-fluorouracilcontaining virus nucleic acid.…”

mentioning

confidence: 99%

The Photochemistry of 5-Fluorouracil^*^†

Lozeron¹,

Gordon²,

Gabriel³

et al. 1964

Biochemistry

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mentioning

confidence: 99%

The Photochemistry of 5-Fluorouracil^*^†

Lozeron¹,

Gordon²,

Gabriel³

et al. 1964

Biochemistry

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“…At a concentration of 12 to 25 ~g/rnl, FU permitted the synthesis of viral antigen by most cells; the same level of FUDR almost completely suppressed the formation of this protein. FU has been found to be incorporated into the RNA of poliovirus (13) and of tobacco mosaic virus (31). Bussard a a/.…”

Section: Discussionmentioning

confidence: 99%

Incomplete Simian Papovavirus Sv40

Melnick

Stinebaugh

Rapp

1964

The Journal of Experimental Medicine

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The successful use of the fluoropyrimidines to gain information about the synthesis of viral DNA (1-3) led us to a study of their effect on the replication of vacuolating virus (SV40). This agent belongs to the papovavirus group 1 (4) which is characterized by 40 to 50 m/~ icosahedral particles possessing a shell of 42 capsomeres and a core of DNA (5, 6). As the time sequence of virus production in the one-step growth cycle had already been determined for SV40 (7), experiments could be readily designed with 5-fluorouracil (FU) and 5-fluorodeoxyuridine (FUDR) to determine whether the synthesis of infectious virus might be suppressed while the formation of viral proteins continued. Reissig and Kaplan (1) had shown that with another icosahedral DNAcontaining virus (swine herpes virus or pseudorabies) grown in the presence of FU, viral protein was assembled into empty particles having the size and shape of herpes virus but without DNA cores. In view of the well known tumorigenic activity of the papovaviruses, and the recent finding that SV40-induced tumors and transformed cells may contain complement-fixing viral antigen in the absence of infectious virus (8), information on the formation of viral antigens in cells which fail to make infectious virus is highly desirable. Materials and MethodsVirus.--The same frozen stock of virus that had been used for determining the growth cycle of SV40 (7) was employed. Its titer was 10 ~'5 plaque-forming units (PFU) per ml.

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“…Pyrimidines halogenated in position 5 have been shown to sensitize bacteria (Kaplan et al, 1962), bacteriophages (Stahl et al, 1961), mammalian cells (Djordjevic and Szybalski, 1960) and RNA viruses (Becarevic et al, 1963) to ultraviolet irradiation. It is interesting to speculate that the phototoxic reaction in nonkeratotic skin and the photopotentiation of the reaction in the keratoses b y sunlight (Dillaha et al, 1965) may be explained b y the increased instability of halogenated RNA and 'thymine-deficient DNA' to ultraviolet light damage.…”

Section: Laboratory Observations and Theoretic Considerationsmentioning

confidence: 99%

Topical therapy with 5‐fluorouracil

Jansen

1971

Journal of Surgical Oncology

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Actinic keratoses are unsightly and may be precancerous. Solitary or localized lesions respond adequately to many forms of treatment. However, widespread multiple actinic keratoses can best be treated with topical 5‐fluorouracil. The exact mode of action is not known, but we can presume it is related to the clearly established effect of this drug upon RNA and DNA synthesis. This treatment is almost ideal, since it usually destroys the keratoses selectively, without significant alteration in normal skin. A 1 or 2 percent solution is made by diluting the 10 ml ampule with propylene glycol. This liquid is applied two times daily to the involved areas for a 2–4 week period. Lesions on the face and neck respond more quickly than those of the hands and arms. A brisk, painful, inflammatory reaction at the site of the keratoses is anticipated and used as an indicator of the end point of treatment. Once treatment is discontinued, the erythema usually subsides in 2 weeks and the skin becomes smooth and free of keratoses. Not all keratoses respond, and untoward reactions such as phototoxicity, irritation of the mucous membranes, contact sensitivity, and alteration of pigmentation can occur. Although resolution of the keratoses persists for years, should they return reapplication is usually effective again.

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Effect of Ultra-violet Light on Tobacco Mosaic Virus containing 5-Fluorouracil

Cited by 21 publications

References 10 publications

The Photochemistry of 5-Fluorouracil^*^†

The Photochemistry of 5-Fluorouracil^*^†

Incomplete Simian Papovavirus Sv40

Topical therapy with 5‐fluorouracil

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Effect of Ultra-violet Light on Tobacco Mosaic Virus containing 5-Fluorouracil

Cited by 21 publications

References 10 publications

The Photochemistry of 5-Fluorouracil*†

The Photochemistry of 5-Fluorouracil*†

Incomplete Simian Papovavirus Sv40

Topical therapy with 5‐fluorouracil

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Product

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The Photochemistry of 5-Fluorouracil^*^†

The Photochemistry of 5-Fluorouracil^*^†