Effect of ultraviolet irradiation on the osseointegration of a titanium alloy with bone

Yadav, Ashish; Yadav, Ravindra Nath; Gupta, Ajay; Baranwal, Akash Kumar; Bhatnagar, Atul; Singh, Vakil

doi:10.4103/ccd.ccd_576_17

Cited by 12 publications

(8 citation statements)

References 25 publications

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“…Titanium is one of the materials mostly used in this field. It has a good biocompatibility, is relatively inert, and is defined by surface features such as topography, roughness, and hydrophilicity that support cell growth and differentiation, influence the protein adsorption of the device implant and its immobilization (Gittens et al, 2014;Liu et al, 2017;Selders et al, 2017;Yadav et al, 2017). One of the primary aims of research in dentistry has been to improve the bone-implant interface and the titanium bioactivity by modifying its surface structure.…”

Section: Introductionmentioning

confidence: 99%

Enhanced VEGF/VEGF-R and RUNX2 Expression in Human Periodontal Ligament Stem Cells Cultured on Sandblasted/Etched Titanium Disk

Marconi

Diomede

Pizzicannella³

et al. 2020

Front. Cell Dev. Biol.

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Bone formation, in skeletal development or in osseointegration processes, is the result of interaction between angiogenesis and osteogenesis. To establish osseointegration, cells must attach to the implant in a direct way without any deposition of soft tissue. Structural design and surface topography of dental implants enhance the cell attachment and can affect the biological response. The aim of the study was to evaluate the cytocompatibility, osteogenic and angiogenic markers involved in bone differentiation of human periodontal ligament stem cells (hPDLSCs) on different titanium disks surfaces. The hPDLSCs were cultured on pure titanium surfaces modified with two different procedures, sandblasted (Control-CTRL) and sandblasted/etched (Test-TEST) as experimental titanium surfaces. After 1 and 8 weeks of culture VEGF, VEGF-R, and RUNX2 expression was evaluated under confocal laser scanning microscopy. To confirm the obtained data, RT-PCR and WB analyses were performed in order to evaluate the best implant surface performance. TEST surfaces compared to CTRL titanium surfaces enhanced cell adhesion and increased VEGF and RUNX2 expression. Moreover, titanium TEST surfaces showed a different topographic morphology that promoted cell adhesion, proliferation, and osteogenic/angiogenic commitment. To conclude, TEST surfaces performed more efficiently than CTRL surfaces; furthermore, TEST surface results showed them to be more biocompatible, better tolerated, and appropriate for allowing hPDLSC growth and proliferation. This fact could also lead to more rapid bone-titanium integration.

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Section: Introductionmentioning

confidence: 99%

Enhanced VEGF/VEGF-R and RUNX2 Expression in Human Periodontal Ligament Stem Cells Cultured on Sandblasted/Etched Titanium Disk

Marconi

Diomede

Pizzicannella³

et al. 2020

Front. Cell Dev. Biol.

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“…It was recently shown that irradiating implants with ultraviolet (UV) light improves their associated cell growth and bone binding ability (osseointegration) [ 1 , 2 ]. Irradiating the surface of titanium (Ti) or titanium alloy (Ti6Al4V) with ultraviolet rays of a short wavelength improves the material’s osseointegration capacity.…”

Section: Introductionmentioning

confidence: 99%

Ultraviolet irradiation improves the hydrophilicity and osteo-conduction of hydroxyapatite

et al. 2020

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Background Treating a titanium or titanium alloy implant with ultraviolet (UV) light is known to improve its associated cell growth and osseointegration. However, little is known about the effect of UV irradiation on hydroxyapatite (HA), which is also used frequently in orthopaedic and dental surgery. Here we examined the effect of UV irradiation on the hydrophilicity of HA, and on its osteoconduction ability in rats. Methods HA implants of low and high porosity were treated with UV light, and photofunctionalisation was assessed by the contact angle of a water drop on the surface. HA implants were also inserted into rat femurs, and the rats were killed 2 or 4 weeks later. The bone volume and bone area ratio were calculated from microcomputed tomography and histological data. Results The contact angle of a water drop on HA implants of both porosities was significantly reduced after UV irradiation. In the rat femurs, there was no significant difference in the bone volume between the UV light-treated and control implants at 2 or 4 weeks. The bone area ratio for the UV light-treated versus control implants was significantly increased at 2 weeks, but there was no significant difference at 4 weeks. Conclusions The surface of UV-irradiated HA disks was hydrophilic, in contrast to that of non-irradiated HA disks. Photofunctionalisation accelerated the increase in the bone area ratio in the early healing stage. This technology can be applied to surgical cases requiring the early fusion of bone and HA.

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“…According to Ashish Yadav [77] the biological medium does not cause major corro- The images of the hFOB 1.19 cells incubated with Ag:HAp/Ti and Ag:Hap + D3/Ti thin films before and after UV irradiation for 24 h emphasized that the hFOB 1.19 cells exhibited a normal morphology and also revealed that the coatings did not induce any significant changes in the hFOB 1.19 cells. The observations made by microscopic visualization of the cells incubated for 24 h with the Ag:HAp/Ti and Ag:Hap + D3/Ti thin films before and after UV irradiation are in good agreement with the quantitative MTT results of the in vitro assay, and evidenced that both all the investigated thin films presents a good biocompatibility and could be suitable for being further employed in the development of biomedical devices.…”

Section: Resultsmentioning

confidence: 98%

“…According to Ashish Yadav [77] the biological medium does not cause major corrosion of titanium and its alloy. On the other hand, previous studies [30][31][32]78] reported that the UV irradiation of the titanium or titanium alloys lead to a significant increase of the surface hydrophilicity and biological properties.…”

Section: Resultsmentioning

confidence: 99%

Multi-Level Evaluation of UV Action upon Vitamin D Enhanced, Silver Doped Hydroxyapatite Thin Films Deposited on Titanium Substrate

et al. 2021

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Hydroxyapatite Ca10(PO4)6(OH)2 (HAp) is an important bioactive material for bone tissue reconstruction, due to its highly thermodynamic stability at a physiological pH without bio-resorption. In the present study, the Ag:HAp and the corresponding Ag:HAp + D3 thin films (~200 nm) coating were obtained by vacuum deposition method on Ti substrate. The obtained samples were exposed to different UV irradiation times, in order to investigate the UV light action upon thin films, before considering this method for the thin film’s decontamination. The effects of UV irradiation upon Ag:Hap + D3 are presented for the first time in the literature, marking a turning point for understanding the effect of UV light on composite biomaterial thin films. The UV irradiation induced an increase in the initial stages of surface roughness of Ag:HAp thin film, correlated with the modifications of XPS and FTIR signals. The characteristics of thin films measured by AFM (RMS) analysis corroborated with XPS and FTIR investigation highlighted a process of recovery of the thin film’s properties (e.g., RMS), suggesting a possible adaptation to UV irradiation. This process has been a stage to a more complicated UVA rapid degradation process. The antifungal assays demonstrated that all the investigated samples exhibited antifungal properties. Moreover, the cytotoxicity assays revealed that the HeLa cells morphology did not show any alterations after 24 h of incubation with the Ag:HAp and Ag:HAp + D3 thin films.

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Effect of ultraviolet irradiation on the osseointegration of a titanium alloy with bone

Cited by 12 publications

References 25 publications

Enhanced VEGF/VEGF-R and RUNX2 Expression in Human Periodontal Ligament Stem Cells Cultured on Sandblasted/Etched Titanium Disk

Enhanced VEGF/VEGF-R and RUNX2 Expression in Human Periodontal Ligament Stem Cells Cultured on Sandblasted/Etched Titanium Disk

Ultraviolet irradiation improves the hydrophilicity and osteo-conduction of hydroxyapatite

Multi-Level Evaluation of UV Action upon Vitamin D Enhanced, Silver Doped Hydroxyapatite Thin Films Deposited on Titanium Substrate

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