Effect of ultraviolet (UV-C) radiation on spores and biofilms of Alicyclobacillus spp. in industrialized orange juice

Prado, Daniela Biral do; Anjos, Márcia Maria dos; Capeloto, Otávio A.; Astrath, N. G. C.; Santos, Naiara Caroline Aparecido dos; Previdelli, Isolde Terezinha Santos; Nakamura, Celso Vataru; Mikcha, Jane Martha Graton; Filho, Benício Alves de Abreu

doi:10.1016/j.ijfoodmicro.2019.108238

Cited by 43 publications

(26 citation statements)

References 22 publications

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“…Studies concerning acidothermophilic bacteria are of great interest and applicable value for juice industry as spores formation and subsequent their germination increases probability of bacterial contamination at the final production steps and products. The control and elimination of Alicyclobacillus biofilms should be adapted to the needs of the production processes [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

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Clove Oil (Syzygium aromaticum L.) Activity against Alicyclobacillus acidoterrestris Biofilm on Technical Surfaces

et al. 2020

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Acidotermophilic bacteria Alicyclobacillus acidoterrestris is one of the main contaminants in the fruit industry forming biofilms which are difficult to remove from the production line by conventional methods. An alternative approach aims for the use of essential oils to prevent Alicyclobacillus biofilm development. The effect of clove essential oil on A. acidoterrestris biofilms on glass and polyvinyl chloride surfaces under static and agitated culture conditions was investigated by atomic force microscopy and the plate count method. The medium-flow and the type of technical surface significantly influenced A. acidoterrestris biofilm. The PVC was colonized in a greater extent comparing to glass. Clove essential oil in 0.05% (v/v) caused 25.1–65.0% reduction of biofilms on the technical surfaces along with substantial changes in their morphology by a decrease in the biofilm: height, surface roughness, and surface area difference. The oil also induced alteration in individual bacterial cells length and visible increase of their roughness. Clove essential oil seems to release EPS from biofilm and thus induce detachment of bacteria from the surface. Due to anti-A. acidoterrestris biofilm activity, the clove oil may be used in the juice industry to hinder a development of A. acidoterrestris biofilms on production surfaces.

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Section: Discussionmentioning

confidence: 99%

“…However, there are some reports concerning Alicyclobacillus’ ability to form biofilms [ 13 , 14 , 36 , 37 ], as the A. acidoterrestris biofilm morphology is still poorly recognized. The literature lacks information on anti-biofilm action of essential oils on these bacteria.…”

Section: Discussionmentioning

confidence: 99%

Clove Oil (Syzygium aromaticum L.) Activity against Alicyclobacillus acidoterrestris Biofilm on Technical Surfaces

et al. 2020

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“…To prepare spore suspension, the methods described by Cai et al (2019b) and Prado et al (2019) were followed with some modifications. A. acidoterrestris vegetative cells were cultured in AAM broth at 45°C for 15 days until about 90% sporulation was observed under a phase contrast microscopy.…”

Section: Methodsmentioning

confidence: 99%

Inactivation Effect of Thymoquinone on Alicyclobacillus acidoterrestris Vegetative Cells, Spores, and Biofilms

et al. 2021

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Alicyclobacillus acidoterrestris (A. acidoterrestris), a spore-forming bacterium, has become a main challenge and concern for the juices and acid beverage industry across the world due to its thermo-acidophilic characteristic. Thymoquinone (TQ) is one of the active components derived from Nigella sativa seeds. The objective of this study was to investigate antibacterial activity and associated molecular mechanism of TQ against A. acidoterrestris vegetative cells, and to evaluate effects of TQ on A. acidoterrestris spores and biofilms formed on polystyrene and stainless steel surfaces. Minimum inhibitory concentrations of TQ against five tested A. acidoterrestris strains ranged from 32 to 64 μg/mL. TQ could destroy bacterial cell morphology and membrane integrity in a concentration-dependent manner. Field-emission scanning electron microscopy observation showed that TQ caused abnormal morphology of spores and thus exerted a killing effect on spores. Moreover, TQ was effective in inactivating and removing A. acidoterrestris mature biofilms. These findings indicated that TQ is promising as a new alternative to control A. acidoterrestris and thereby reduce associated contamination and deterioration in the juice and acid beverage industry.

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“…(2) chemical [56][57][58]; (3) physical [59,60]; (4) physicochemical [61][62][63]; (5) physical in combination with natural [64], and (6) natural in combination with chemical [65]. A summary of the bacterial biofilm inhibition/control methods described in the past two years is shown in Figure 1.…”

Section: Biofilm Control Methodsmentioning

confidence: 99%

“…In order not to exclude search results that could be significant for the investigation, a general search term (keyword) "biofilm" was used. According to the scientific articles found in the PubMed database regarding biofilm inhibition and control, all methods, based on their nature, can be divided into six groups: (1) natural (biological) [53][54][55]; (2) chemical [56][57][58]; (3) physical [59,60]; (4) physicochemical [61][62][63]; (5) physical in combination with natural [64], and (6) natural in combination with chemical [65]. A summary of the bacterial biofilm inhibition/control methods described in the past two years is shown in Figure 1.…”

Section: Biofilm Control Methodsmentioning

confidence: 99%

Antimicrobial Photoinactivation Approach Based on Natural Agents for Control of Bacteria Biofilms in Spacecraft

Buchovec

Gricajeva

Kalėdienė

et al. 2020

IJMS

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A spacecraft is a confined system that is inhabited by a changing microbial consortium, mostly originating from life-supporting devices, equipment collected in pre-flight conditions, and crewmembers. Continuous monitoring of the spacecraft’s bioburden employing culture-based and molecular methods has shown the prevalence of various taxa, with human skin-associated microorganisms making a substantial contribution to the spacecraft microbiome. Microorganisms in spacecraft can prosper not only in planktonic growth mode but can also form more resilient biofilms that pose a higher risk to crewmembers’ health and the material integrity of the spacecraft’s equipment. Moreover, bacterial biofilms in space conditions are characterized by faster formation and acquisition of resistance to chemical and physical effects than under the same conditions on Earth, making most decontamination methods unsafe. There is currently no reported method available to combat biofilm formation in space effectively and safely. However, antibacterial photodynamic inactivation based on natural photosensitizers, which is reviewed in this work, seems to be a promising method.

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Effect of ultraviolet (UV-C) radiation on spores and biofilms of Alicyclobacillus spp. in industrialized orange juice

Cited by 43 publications

References 22 publications

Clove Oil (Syzygium aromaticum L.) Activity against Alicyclobacillus acidoterrestris Biofilm on Technical Surfaces

Clove Oil (Syzygium aromaticum L.) Activity against Alicyclobacillus acidoterrestris Biofilm on Technical Surfaces

Inactivation Effect of Thymoquinone on Alicyclobacillus acidoterrestris Vegetative Cells, Spores, and Biofilms

Antimicrobial Photoinactivation Approach Based on Natural Agents for Control of Bacteria Biofilms in Spacecraft

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