Effect of vaporized perfluorocarbon on oxidative stress during the cold ischemia phase of lung graft preservation

Abstract

Liquid perfluorocarbon (PFC) instillation has been studied experimentally as an adjuvant therapy in the preservation of lung grafts during cold ischemia. The objective of this study was to evaluate whether vaporized PFC is also protective of lung grafts at different cold ischemia times. We performed histological analysis of and measured oxidative stress in the lungs of animals that received only preservation solution with low-potassium dextran (LPD) or vaporized PFC together with LPD. We conclude that vaporized PFC reduces the production of free radicals and the number of pulmonary structural changes resulting from cold ischemia.

Mammalian enteral ventilation ameliorates respiratory failure

“…[11][12][13] For example, when an emulsified form of perfluorochemicals was administered directly into the blood vessels or the airway, relatively modest side effects were reported, including the elevation of blood pressure and injury to organs. 12,[17][18][19] The level of arterial oxygenation, if scaled for human application, is likely sufficient to alleviate patients with severe respiratory failure. -PFD potentially provides lifesaving oxygenation, while there is additional room for improving gas exchange efficacy.…”

Research Progress in Oxygen Carrier Design and Application

“…At the same time, PFC is easily distributed throughout the lungs in a more uniform manner during the vaporization process and will not cause lung ventilation difficulties caused by infusion of liquid PFC. In a cold ischemia rat model, vaporized PFC + LPD preserved lungs exhibited higher superoxide dismutase (SOD) concentrations than LPD alone, suggesting that use of vaporized PFC reduces free radical production, which in turn protects lung grafts from oxidative stress damage …”

“…In a cold ischemia rat model, vaporized PFC + LPD preserved lungs exhibited higher superoxide dismutase (SOD) concentrations than LPD alone, suggesting that use of vaporized PFC reduces free radical production, which in turn protects lung grafts from oxidative stress damage. 103 3.2.3. Application of PFC Oxygen Carriers in Tumor Therapy.…”

Systemic Redox Status of Rats Treated with Different Doses of Perfluorocarbon Based Blood Substitute- Perftoran®

“…In previous studies, PFT was mentioned as potential preventive strategy for ischemia/reperfusion injuries for example in lung graft preservation (14). Also, it is suggested that PFT could decrease production of inflammatory markers and stress oxidative markers, decrease lung oedema and hydroxyproline level, as well as attenuate histopathological changes and fibrosis (15).…”