Effect of variants in the ovine skeletal-muscle-specific calpain gene on body weight

Abstract

The ovine skeletal-muscle-specific calpain gene (p94), which is known also as the n-calpain or calpain 3 gene (CAPN3), was screened with primers. Selection of the PCR primers was based on the ovine cDNA sequence (GenBank accession No. AF087570). After sequence alignment between the ovine and human (AY902237) genes, exon and intron boundaries were determined. Polymorphisms were observed in the intron region for the CAPN31112 and CAPN31213 segments, and the sequences for these segments were submitted to the GenBank (AF309635 and AY102617, respectively). Body weight was recorded at birth, weaning and post-weaning. Calpain 3 genotypes of the CAPN31112 segment were associated with birth weight (P < 0.01), and a dominant gene effect was observed. Breeding group, birth type, and rearing type were significantly associated with weight traits. Allele frequencies were similar in purebred and crossbred animals.

Variation in Exon 10 of the Ovine Calpain3 Gene and Its Association With Growth and Carcass Traits in Egyptian Barki Lambs

“…There are few studies linking CAPN3 genotypes to growth traits and market retail cuts of lambs. These studies reported that CAPN3 polymorphisms were associated with increased (11.8 to 13.3%) lamb birth weight (Chung et al, 2007), increased (1.8 to 2.4%) leg weight (Bickerstaffe et al, 2008) and increased shoulder weight and hot carcass weight (Fang et al, 2013). In our study, we found that polymorphisms in CAPN3 gene had significant association with post-weaning daily gain, marketing weight and lean meat%.…”

“…In sheep, a single nucleotide polymorphism (SNP) in intron 11 of CAPN3 gene has been associated with birth weight (Chung et al, 2007), and the yield of retail lamb meat cuts (Bickerstaffe et al, 2008). The allelic variants, which detected in exon 10 by Fang et al (2013), were found to be associated with shoulder weight and hot carcass weight (P< 0.05).…”

A neural network model to describe weight gain of sheep from genes polymorphism, birth weight and birth type

“…Quantitative traits such as average daily gain (ADG) or final body weight are controlled by a great number of genes. Several genes such as growth hormone (GH), leptin, calpain, and calpastatin are considered as genetic candidate markers for growth rate and meat production traits in mammalian (Chung et al, 2007;Marques et al, 2006;Nassiry et al, 2007;Zhou et al, 2009). Investigation into the relationship between these genetic markers polymorphism and ADG of lambs may lead to design a more efficient selection program.…”

Association of the single nucleotide polymorphism in CAPN3 gene with growth performance in Merino and Garut (MEGA) backcross sheep

“…The SSCP pattern showed three allele sequences of CAPN3 in sheep, and single nucleotide polymorphism (SNP) was identified at exon 10 [ 1 ]. Furthermore, CAPN3 SNP on intron 11 was found to be associated with birth weight in sheep [ 4 ].…”