Effect of various plant growth regulators on in vitro seed germination and shoot organogenesis in Tectona grandis L.f.

Mishra, Jay Prakash; Bhadrawale, Deepti; Yadav, Upasana; Mohammad, Naseer; Shirin, Fatima

doi:10.22271/tpr.2018.v5.i2.020

Cited by 4 publications

(7 citation statements)

References 9 publications

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“…Root tips from in vitro raised seedlings of Citrus indica cultured on full strength and half strength MS supplemented with 2,4-D, IBA, NAA, Kn and BAP in varying concentrations (0.25 -1.5 mg/l) and combinations did not show shoot initiation even after one month of observation. Similarly, Mishra et al (2018) tested various explants viz., leaf, internode, hypocotyls and root of seedlings of Tectona grandis for shoot organogenesis and observed that the root explants were least responsive.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of explants for in vitro propagation of Citrus indica Tanaka - An Endangered Species

Sangma

Pereira

Dang³

et al. 2020

Plant Tissue Cult. & Biotech.

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Of the five explants viz., shoot tip, nodal segment, leaf disc, cotyledon and root tip excised from exegenic seedlings of Citrus indica Tanaka shoot tip cultured in MS supplemented with 0.5 mg/l of BAP generated shoots within 4.74 days, exhibiting highest percentage of response (85.82%) with highest number of shoots (8.9) and shoot length (3.04 cm). On the other hand nodal segment cultured in MS supplemented with 1.0 mg/l BAP showed 80% response in 5.16 days with a shoot number of 5.41 and shoot length of 2.43 cm. Cotyledon explants inoculated on MS supplemented with1.0 mg/l of TDZ produced shoots in 20 days with the highest response of 69.88%, with 3.77 shoots per cotyledon and shoot length of 2.03cm. Viable callus was obtained from leaf disc cultured on half strength MS medium with less Ca++ with 2, 4-D 0.5 mg/l + Kn 0.25 mg/l. This callus when inoculated on half strength MS medium with Kn 1.5 mg/l showed highest shoot bud proliferation of 66.66% with 10.06 shoots per callus. Root tip explant failed to produce any shoots. In vitro raised shoots of Citrus indica when cultured on half strength MS medium supplemented with NAA (1.0 mg/l) showed 80 % rooting in 5.66 days, with highest number of roots (6.16 per shoot) and longest root (3.78cm). Ninety per cent of in vitro rooted plantlets of Citrus indica survived in open conditions. Plant Tissue Cult. & Biotech. 30(1): 87-96, 2020 (June)

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Section: Resultsmentioning

confidence: 99%

Evaluation of explants for in vitro propagation of Citrus indica Tanaka - An Endangered Species

Sangma

Pereira

Dang³

et al. 2020

Plant Tissue Cult. & Biotech.

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“…Mercuric chloride atau merkuri biasanya digunakan untuk mendapatkan eksplan steril dari benih dan tunas jati (Kozgar & Shahzad, 2012;Antony et al, 2015;Fauzan, Supriyanto, & Tajuddin, 2017;Mishra, Bhadrawale, & Yadav, 2018). Merkuri jika dibuang langsung ke tanah akan mencemari lingkungan, maka diperlukan bahan sterilan yang lebih ramah lingkungan.…”

Section: Pendahuluanunclassified

“…Beberapa penelitian menggunakan larutan merkuri 0,1%-1% dengan waktu perendaman antara 3 -5 menit untuk sterilisasi permukaan (Kozgar & Shahzad, 2012;Mishra et al, 2018 ( Ivanova & Van Staden, 2008;Rahman, Haider, Hossain, & Islam, 2011;Sianturi et al, 2017). Pada media modifikasi konsentrasi KNO3 (kalium nitrat) lebih banyak dibandingkan dengan NH4NO3 (ammonium nitrat).…”

Section: Analisis Dataunclassified

Selection of the Optimum Seed and Media Sterilization Techniques for Muna Teak (Tectona grandis L.) Micropropagation

Sianturi¹,

Bramasto

Yuniarti

et al. 2020

J.Perbenihan.Tan.Hutan

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The existence of the muna teak (Tectona grandis L.), is currently threatened with extinction, so a muna teak replanting program is needed. Micropropagation techniques can be one solution for the supply of high quality seedlings. The purpose of this study was to obtain the optimum sterilization technique to get the sterilize explants of muna teak and to get the suitable media for in vitro multiplication. The study was conducted in a tissue culture laboratory of the Forest Tree Seed Technology Research and Development Centre in Bogor. This study consisted of two stages and the experimental design used was a completely randomized design. The first phase of the study was the treatment of sterilization techniques consisted of soaking explants in various concentration of clorox solution and the second stage of the study was the treatment of multiplication media. The results showed that the proper sterilization technique to obtain sterilize explants of muna teak seedlings was immersed in 20% chlorox solution for 10 minutes, then immersed in 15% of chlorox for 15 minutes. The best medium for the induction stage and multiplication of Muna teak buds which are modified media plus Benzyl Amino Purine (BAP) hormone as much as 0.1 mg.l -1

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“…Another tissue culture utilization opportunity is to be able to cross distantly related species to regenerate hybrid novels through protoplast fusion, production of double monoploid crops with kolkisin treatment from haploid cultures to reach homozygous strains faster in breeding programs, seed production of certain plants that require a high level of genetic conservation for seed production, as a better scope and future for the production of important phytopharmaceuticals, as well as helping the successful production of valuable, specific plant chemicals that have not yet been discovered. Successful propagation of tissue cultures of woody plants has been widely reported, among others, in the species Tectona grandis [34], Santalum album [29], Acacia sinuate [18] and Aquilaria malaccensis Lam [30]. Separate screenings related to the influence of seasons [3,5,6,8,21] basic media [16,35] and growth-stimulating substances [13,14,34] have been widely reported as well as the results of comprehensive screening related to the three factors mentioned above [1,2,4,9,12,13,18,20,22,23,[25][26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

“…Successful propagation of tissue cultures of woody plants has been widely reported, among others, in the species Tectona grandis [34], Santalum album [29], Acacia sinuate [18] and Aquilaria malaccensis Lam [30]. Separate screenings related to the influence of seasons [3,5,6,8,21] basic media [16,35] and growth-stimulating substances [13,14,34] have been widely reported as well as the results of comprehensive screening related to the three factors mentioned above [1,2,4,9,12,13,18,20,22,23,[25][26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Season, basal media and plant growth regulators effect in wood plant in vitro propagation: a comprehensive review

Jayusman

Hakim

Dalimunthe

2022

IOP Conf. Ser.: Earth Environ. Sci.

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Wood plant in-vitro propagation is currently widely used and is not limited to the preparation of superior seeds but has been widely utilized for the production of phytopharmaceuticals and the production of a valuable and specific tree for the benefit of man. Studies focused on the influence of explant collection seasons, selection of basic media, and plant growth hormone. The results of the study showed the magnitude of the variables that must be considered in the propagation of tissue cultures even in the same species and types of cultures. The period of January and March is the best time for explant retrieval because it provides the best explant response in bud breakage, lowest browning, and best bud induction. The basic media of MS (Murashige & Skoog), as well as its various modifications and the basic medium of WPM (Wood Plant Medium), tend to be widely chosen as the basic media at various levels of subcultures in many woody plant species. The application of growth regulators shows a tendency to use BAP (benzyl amino purine) and NAA (naphthalene acetic acid) whose application is separately or in combination with a certain concentration according to the species and subculture phase. Other plant growth regulators (PGRs) that are also quite widely used are the IBA (indole butyric acid), IAA (indole acetic acid), and G3 (gibberellic acid) groups which are used in the multiplication and rooting stages. The information from the study can be used as basic information at the stage of optimizing tissue culture activities in different species, explanatory, and regeneration types. The success of the optimization stage will be used as an improvement to the previous woody plant tissue culture protocol so that optimization of success is achieved. In vitro propagation is always associated with the number of resources used, so efforts to improve in vitro protocols are a very important activity to evaluate that will lead to increased effectiveness to reduce resource inputs, and be able to realize low-cost wood plant tissue culture propagation.

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Effect of various plant growth regulators on in vitro seed germination and shoot organogenesis in Tectona grandis L.f.

Cited by 4 publications

References 9 publications

Evaluation of explants for in vitro propagation of Citrus indica Tanaka - An Endangered Species

Evaluation of explants for in vitro propagation of Citrus indica Tanaka - An Endangered Species

Selection of the Optimum Seed and Media Sterilization Techniques for Muna Teak (Tectona grandis L.) Micropropagation

Season, basal media and plant growth regulators effect in wood plant in vitro propagation: a comprehensive review

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