Effect of varying feedstock–pretreatment chemistry combinations on the formation and accumulation of potentially inhibitory degradation products in biomass hydrolysates

Du, Bowen; Sharma, Lekh N.; Becker, Christopher H.; Chen, Shou‐Feng; Mowery, Richard A.; Walsum, G. Peter van; Chambliss, C. Kevin

doi:10.1002/bit.22829

Cited by 204 publications

(149 citation statements)

References 57 publications

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“…S1A). To probe which component in the highly heterogeneous PCS liquor potentiates GH61s, individual liquor components (19) were incubated together with TaGH61 and assessed for their ability to cleave cellulose. From these experiments, gallate (gallic acid) was shown to increase the activity of GH61 enzymes in the degradation of microcrystalline cellulose by H. jecorina cellulases (Fig.…”

Section: Resultsmentioning

confidence: 99%

Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components

Quinlan

Sweeney

Leggio

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

910

1,144

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The enzymatic degradation of recalcitrant plant biomass is one of the key industrial challenges of the 21st century. Accordingly, there is a continuing drive to discover new routes to promote polysaccharide degradation. Perhaps the most promising approach involves the application of "cellulase-enhancing factors," such as those from the glycoside hydrolase (CAZy) GH61 family. Here we show that GH61 enzymes are a unique family of copper-dependent oxidases. We demonstrate that copper is needed for GH61 maximal activity and that the formation of cellodextrin and oxidized cellodextrin products by GH61 is enhanced in the presence of small molecule redox-active cofactors such as ascorbate and gallate. By using electron paramagnetic resonance spectroscopy and single-crystal X-ray diffraction, the active site of GH61 is revealed to contain a type II copper and, uniquely, a methylated histidine in the copper's coordination sphere, thus providing an innovative paradigm in bioinorganic enzymatic catalysis.ellulose is Earth's most abundant biopolymer. Its exploitation as an energy source plays a critical role in the global ecology and carbon cycle. Industrial production of fuels and chemicals from this plentiful and renewable resource holds the potential to displace petroleum-based sources, thus reducing the associated economic and environmental costs of oil and gas production (1, 2) and promoting energy security as part of a balanced energy portfolio. However, despite the burgeoning potential of cellulose as a biofuel source, its remarkable recalcitrance to depolymerization has so far hindered the economical use of any form of lignocellulosic biomass as a feedstock for biofuel production (3, 4).In addressing the issue of cellulose recalcitrance, much effort has been directed toward harnessing the known cellulosedegrading enzymatic pathways found in fungi. The consensus model of enzymatic degradation involves the concerted action of a consortium of different endoglucanases and "exo"-acting cellobiohydrolases (collectively termed "cellulases"); both enzyme classes perform classical glycoside hydrolysis through attack of water at the anomeric center of oligo/polysaccharide substrates (5-9). Necessarily as part of the overall enzymatic degradation of cellulose, the initial enzymatic step must overcome cellulose's inertness by disrupting the cellulosic structure, thus allowing attack by traditional cellulases. Originally, Reese et al. (10) suggested that undefined enzymes could play a major role in this step. This notion remained a hypothesis until very recently when, in a key paper, Harris et al. (11) demonstrated that inclusion of a novel enzyme class, currently termed GH61 glycoside hydrolases in the CAZy database of carbohydrate-active enzymes (12), greatly increases the performance of Hypocrea jecorina (Trichoderma reesei) cellulases in lignocellulose hydrolysis. From this work, it was suggested that GH61s act directly on cellulose rendering it more accessible to traditional cellulase action (11). Moreover, recent genomi...

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Section: Resultsmentioning

confidence: 99%

Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components

Quinlan

Sweeney

Leggio

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

910

1,144

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“…Different analytical techniques, primarily gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS), have been used to identify specific aromatic compounds in acidic hydrolysates from various kinds of lignocellulosic feedstocks, such as corn stover [24][25][26], oak [27], pine [26,28,29], poplar [24,[30][31][32], spruce [33][34][35], sugarcane bagasse [22], switchgrass [24], and willow [36]. In addition, aromatic degradation products in hydrolysates produced by alkaline methods have been investigated [26,37].…”

Section: Aromatic Compoundsmentioning

confidence: 99%

- Synthetic Biology: A Promising Technology for Biofuel Production

2015

New Biotechnologies for Increased Energy Security

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“…Phenolic compounds derived from lignin are also potentially harmful to fermentation operations. From these data, it is clear that inhibitory furans are produced most abundantly at low pH, suggesting that higher pH acid hydrolysis could be used to limit production of these compounds [27].…”

Section: -1 Degradation Chemicals As Function Of Phmentioning

confidence: 98%

“…Understanding the various formation and accumulation trends of furans is particularly relevant as these compounds are known to have significant inhibitory effects [27]. Phenolic compounds derived from lignin are also potentially harmful to fermentation operations.…”

Section: -1 Degradation Chemicals As Function Of Phmentioning

confidence: 99%

Optimization and Evaluation of Organic Acid Recovery from Kraft Black Liquor Using Liquid-Liquid Extraction

Kwon¹,

Um²

2016

Korean Chemical Engineering Research

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− Liquid-liquid extraction (LLE) can be used for the recovery of acetic acid from black liquor prior to bioethanol fermentation. Recovery of value-added chemicals such as acetic-, formic-and lactic acid using LLE from Kraft black liquor was studied. Acetic acid and formic acid have been reported to be strong inhibitors in fermentation. The study elucidates the effect of three reaction parameters: pH (0.5~3.5), temperature (25~65 o C), and reaction time (24~48 min). Extraction performance using tri-n-octylphosphine oxide as the extractant was evaluated. The maximum acetic acid concentration achieved from hydrolyzates was 69.87% at 25°C, pH= 0.5, and 36 min. Factorial design was used to study the effects of pH, temperature, and reaction time on the maximum inhibitor extraction yield after LLE. The maximum potential extraction yield of acetic acid was 70.4% at 25.8°C, pH=0.6 and 37.2 min residence time.

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Effect of varying feedstock–pretreatment chemistry combinations on the formation and accumulation of potentially inhibitory degradation products in biomass hydrolysates

Cited by 204 publications

References 57 publications

Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components

Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components

- Synthetic Biology: A Promising Technology for Biofuel Production

Optimization and Evaluation of Organic Acid Recovery from Kraft Black Liquor Using Liquid-Liquid Extraction

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