Effect of vehicles on yohimbine permeation across excised hairless mouse skin

Carelli, V.; Colo, Giacomo Di; Nannipieri, E.; Serafini, M.F.

doi:10.1016/s0031-6865(98)00007-7

Cited by 7 publications

(1 citation statement)

References 16 publications

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“…The SC lipid transition at 59 C (T 1 ) and 65 C (T 2 ) were not appeared from the DSC thermogram of negative control SC samples ( Figure 6B) with a broad new transition at 77.52 C. DSC results demonstrated that efficient conformational changes had been developed by the AmB gel formulation on SC lipids leading new responsible characteristics endothermic transition peaks at T 1 (59 C) and T 2 (65 C). The absence of peaks T 1 and T 2 in both the negative control skin samples and AmB-NE gel treated verified the intercellular lipid transition associated with the skin (Zellmer et al, 1995;Carelli et al, 1998;Changez, et al, 2006). Hence, our results suggested that AmB-NE gel caused significant modification in the lipid architectural structures of SC by lipids dissolution.…”

Section: Ex Vivo Globule-skin Interactionsupporting

confidence: 68%

Nanoemulsion gel-based topical delivery of an antifungal drug:in vitroactivity andin vivoevaluation

Samad²,

et al. 2014

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Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard University, New Delhi, India AbstractObjective: In this study, attempt has been focused to prepare a nanoemulsion (NE) gel for topical delivery of amphotericin B (AmB) for enhanced as well as sustained skin permeation, in vitro antifungal activity and in vivo toxicity assessment. Materials and methods: A series of NE were prepared using sefsol-218 oil, Tween 80 and Transcutol-P by slow spontaneous titration method. Carbopol gel (0.5% w/w) was prepared containing 0.1% w/w AmB. Furthermore, NE gel (AmB-NE gel) was characterized for size, charge, pH, rheological behavior, drug release profile, skin permeability, hemolytic studies and ex vivo rat skin interaction with rat skin using differential scanning calorimeter. The drug permeability and skin irritation ability were examined with confocal laser scanning microscopy and Draize test, respectively. The in vitro antifungal activity was investigated against three fungal strains using the well agar diffusion method. Histopathological assessment was performed in rats to investigate their toxicological potential.Results and discussion: The AmB-NE gel (18.09 ± 0.6 mg/cm 2 /h) and NE (15.74 ± 0.4 mg/cm 2 /h) demonstrated the highest skin percutaneous permeation flux rate as compared to drug solution (4.59 ± 0.01 mg/cm 2 /h) suggesting better alternative to painful and nephrotoxic intravenous administration. Hemolytic and histopathological results revealed safe delivery of the drug. Based on combined results, NE and AmB-NE gel could be considered as an efficient, stable and safe carrier for enhanced and sustained topical delivery for AmB in local skin fungal infection. Conclusion: Topical delivery of AmB is suitable delivery system in NE gel carrier for skin fungal infection.

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Section: Ex Vivo Globule-skin Interactionsupporting

confidence: 68%