Vibration during welding can be used to obtain certain changes in mechanical properties of weld metal. Research work on the influence of vibration on the secondary microstructure of welds and hence on the Charpy toughness was performed. Vibration during welding exhibits positive effects on the microstructure constituent formation. Multipass welding was simulated with reheating of the original single pass weld in order to obtain similar microstructure to multipass welds. Microstructures were examined with an optical microscope. Additionally, fractographic examination of the rupture of Charpy specimens was performed. Changes in the microstructure according to vibration were observed which affect toughness of the weld metal. Vibration during welding was rated more effective in the case of reheating the weld metal, which is the case in multipass welding.