Effect of Virazole (Ribavirin) on tomato spotted wilt virus in two systemic hosts, tomato and tobacco

Fazio, G. de; Caner, J.; Vicente, M.

doi:10.1007/bf01315037

Cited by 23 publications

(5 citation statements)

References 12 publications

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“…The antiviral activity of ribavirin was shown for plant viruses such as tomato white necrosis virus (De Fasio et al, 1978), tobacco mosaic virus (Dawson and Lozolya-Saldana, 1984), potato virus X (Shepard, 1975;Lerch, 1987) as well as for the animal hepatitis C virus (Lunel et al, 2003). Ribavirin inhibits an early step of virus replication (De Fazio et al, 1980;Dawson and Lozolya-Saldana, 1984) and induces mutations in virus polymerase (Vo et al, 2003). Applying ribavirin (10 mg/l) in the regeneration medium allowed us to eliminate PVX from 87% to 93% of the regenerated plants (Table 1).…”

Section: Discussionmentioning

confidence: 97%

PVX-Cre-mediated marker gene elimination from transgenic plants

Kopertekh¹,

Jüttner²,

Schiemann³

2004

Plant Mol Biol

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Cre recombinase gene from bacteriophage P1 was transiently expressed by a Potato Virus X (PVX)-based vector in transgenic lox-target Nicotiana benthamiana plants to remove the selectable marker gene. The target construct consisted of two directly oriented lox sites flanking a bar gene located between a gfp coding region and an upstream CaMV 35S promoter. The Cre-mediated excision of intervening sequence placed the gfp coding region under the transcriptional control of the CaMV 35S promoter. GFP activity was observed in PVX-Cre systemically infected leaves, regenerants from PVX-Cre infected explants and T 1 progeny of these regenerants. PVX-Cre was removed efficiently from the regenerants by adding the nucleoside analogue ribavirin to the culture medium. Molecular data proved a correlation between gfp expression and precise site-specific excision of the bar gene in all examined transgenic lines. The frequency of recombination expressed as a percentage of regenerated plants exhibiting marker gene excision varied from 48% to 82%. These results demonstrate that a plant virus vector can be used efficiently to express cre recombinase in vivo providing an alternative method for the production of transgenic plants without marker genes.

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Section: Discussionmentioning

confidence: 97%

PVX-Cre-mediated marker gene elimination from transgenic plants

Kopertekh¹,

Jüttner²,

Schiemann³

2004

Plant Mol Biol

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“…Plant viral diseases are frequently emerging primarily due to the changing pattern of climatic variables, trading of commodity, and the plant viruses evolving more rapidly [4]. During the 1980s, approximately USD 15-20 billion loss was caused by plant viral diseases [5] and more than USD 30 billion in 2014 [6]. This also contributes to intensifying the global economic impact of plant virus disease [7].…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of Microbial Plant Protection and Control of Plant Viruses

Manjunatha

Rajashekara

Uppala

et al. 2022

Plants

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Plant viral diseases are major constraints causing significant yield losses worldwide in agricultural and horticultural crops. The commonly used methods cannot eliminate viral load in infected plants. Many unconventional methods are presently being employed to prevent viral infection; however, every time, these methods are not found promising. As a result, it is critical to identify the most promising and sustainable management strategies for economically important plant viral diseases. The genetic makeup of 90 percent of viral diseases constitutes a single-stranded RNA; the most promising way for management of any RNA viruses is through use ribonucleases. The scope of involving beneficial microbial organisms in the integrated management of viral diseases is of the utmost importance and is highly imperative. This review highlights the importance of prokaryotic plant growth-promoting rhizobacteria/endophytic bacteria, actinomycetes, and fungal organisms, as well as their possible mechanisms for suppressing viral infection in plants via cross-protection, ISR, and the accumulation of defensive enzymes, phenolic compounds, lipopeptides, protease, and RNase activity against plant virus infection.

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“…The most common and important viruses of potato are potato leaf roll virus (PLRV), potato virus Y (PVY), potato virus A (PVA), potato virus X (PVX), potato virus S (PVS), and potato virus M (PVM). Currently, viruses cannot be controlled with chemical pesticides, since known antiviral compounds such as teratogenic ribavirin (1,β- D -ribofuranosyl-l,2,4-triazole-3-carboxamide) are hazardous to people’s health ( De Fazio et al, 1980 ). Considering that viruses have more than one species of plant host and that several unrelated viruses can infect plants simultaneously, it is important that more than one virus can be found within the same plant and can damage crops to a greater degree ( Moreno and López-Moya, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Endophytic Bacillus spp. as a Prospective Biological Tool for Control of Viral Diseases and Non-vector Leptinotarsa decemlineata Say. in Solanum tuberosum L.

et al. 2020

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Viral diseases and their damage causing significant loss to economically important crops have increased by several folds during the last decade. All the conventional approaches are not able to eradicate the viral infection. Therefore, there is a need to look for efficient and eco-friendly viral disease-preventive measures. The genomic material of the majority of deleterious viruses of higher plants is RNA. One of the possible measures to control viruses is the use of ribonucleases (RNases), which can cleave RNA in the viral genome. Based on this, we investigated the RNase activity of endophytic Bacillus spp., which can enrich in 10 3 –10 5 colony-forming units per gram of wet mass of aboveground part of potato plants. A high level of RNase activity was observed in the culture medium of Bacillus thuringiensis B-6066, Bacillus sp. STL-7, Bacillus sp. TS2, and Bacillus subtilis 26D. B. thuringiensis B-5351 had low RNase activity but high ability to colonize internal plant tissues, Bacillus sp. STL-7 with high RNase activity have relatively low number of cells in internal tissues of plants. B. thuringiensis B-6066, B. subtilis 26D, and Bacillus sp. TS stimulate RNase activity in potato plants for a long time after application. Strains with high ability to colonize internal plant tissues combined with high RNase activity reduced severity of viral diseases symptoms on plants and reduced the incidence of potato viruses M, S, and Y. It is worth noting that Bacillus spp. under investigation reduced the number of Leptinotarsa decemlineata Say. egg clusters and larvae on treated plants and showed antifeedant activity. This results in increase of potato productivity mainly in the fraction of major tubers. B. subtilis 26D and Bacillus sp. TS2 combining endophytic lifestyle, RNase, and antifeedant activity may become the basis for the development of biocontrol agents for plant protection.

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Effect of Virazole (Ribavirin) on tomato spotted wilt virus in two systemic hosts, tomato and tobacco

Cited by 23 publications

References 12 publications

PVX-Cre-mediated marker gene elimination from transgenic plants

PVX-Cre-mediated marker gene elimination from transgenic plants

Mechanisms of Microbial Plant Protection and Control of Plant Viruses

Endophytic Bacillus spp. as a Prospective Biological Tool for Control of Viral Diseases and Non-vector Leptinotarsa decemlineata Say. in Solanum tuberosum L.

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