Mechanisms of Microbial Plant Protection and Control of Plant Viruses

Manjunatha, L.; Rajashekara, H.; Uppala, Leela Saisree; Ambika, Dasannanamalige Siddesh; Patil, Balanagouda; Shankarappa, K. S.; Nath, Vishnu Sukumari; Kavitha, Tiptur Rooplanaik; Mishra, Ajay Kumar

doi:10.3390/plants11243449

Cited by 29 publications

(9 citation statements)

References 203 publications

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“…Niastella can promote plant growth and defense against pathogens ( Cuartero et al, 2022 ). Overall, the beneficial microorganisms were consistent with the previous studies ( Manjunatha et al, 2022 ).…”

Section: Discussionsupporting

confidence: 91%

Insight into the soil bacterial community succession of Nicotiana benthamiana in response to Tobacco mosaic virus

Zhao,

Liu,

et al. 2024

Front. Microbiol.

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BackgroundTobacco mosaic virus (TMV) is one famous plant virus responsible for substantial economic losses worldwide. However, the roles of bacterial communities in response to TMV in the tobacco rhizosphere remain unclear.MethodsWe explored the soil physicochemical properties and bacterial community succession of the healthy (YTH) and diseased (YTD) plants with TMV infection by 16S rRNA gene sequencing and bioinformatics analysis.ResultsWe found that soil pH in the YTD group was significantly lower than in the YTH group, and the soil available nutrients were substantially higher. The bacterial community analysis found that the diversity and structure significantly differed post-TMV disease onset. With TMV inoculated, the alpha diversity of the bacterial community in the YTD was markedly higher than that in the YTH group at the early stage. However, the alpha diversity in the YTD group subsequently decreased to lower than in the YTH group. The early bacterial structure of healthy plants exhibited higher susceptibility to TMV infection, whereas, in the subsequent stages, there was an enrichment of beneficial bacterial (e.g., Ramlibacter, Sphingomonas, Streptomyces, and Niastella) and enhanced energy metabolism and nucleotide metabolism in bacteria.ConclusionThe initial soil bacterial community exhibited susceptibility to TMV infection, which might contribute to strengthening resistance of Tobacco to TMV.

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Section: Discussionsupporting

confidence: 91%

Insight into the soil bacterial community succession of Nicotiana benthamiana in response to Tobacco mosaic virus

Zhao,

Liu,

et al. 2024

Front. Microbiol.

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“…Plant pathogenic viruses pose a serious threat to livestock as well as agricultural, horticultural, and ornamental crops [ 37 , 38 ]. The Potato virus Y detected in this study belongs to the RNA-containing virus family Potyvirus of the family Potyviridae , which represents approximately 30% of all known plant pathogenic viruses.…”

Section: Discussionmentioning

confidence: 99%

The Metagenomic Analysis of Viral Diversity in Colorado Potato Beetle Public NGS Data

Starchevskaya

Kamanova

Vyatkin

et al. 2023

Viruses

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The Colorado potato beetle (CPB) is one of the most serious insect pests due to its high ecological plasticity and ability to rapidly develop resistance to insecticides. The use of biological insecticides based on viruses is a promising approach to control insect pests, but the information on viruses which infect leaf feeding beetles is scarce. We performed a metagenomic analysis of 297 CPB genomic and transcriptomic samples from the public National Center for Biotechnology Information Sequence Read Archive (NCBI SRA) database. The reads that were not aligned to the reference genome were assembled with metaSPAdes, and 13314 selected contigs were analyzed with BLAST tools. The contigs and non-aligned reads were also analyzed with Kraken2 software. A total of 3137 virus-positive contigs were attributed to different viruses belonging to 6 types, 17 orders, and 32 families, matching over 97 viral species. The annotated sequences can be divided into several groups: those that are homologous to genetic sequences of insect viruses (Adintoviridae, Ascoviridae, Baculoviridae, Dicistroviridae, Chuviridae, Hytrosaviridae, Iflaviridae, Iridoviridae, Nimaviridae, Nudiviridae, Phasmaviridae, Picornaviridae, Polydnaviriformidae, Xinmoviridae etc.), plant viruses (Betaflexiviridae, Bromoviridae, Kitaviridae, Potyviridae), and endogenous retroviral elements (Retroviridae, Metaviridae). Additionally, the full-length genomes and near-full length genome sequences of several viruses were assembled. We also found sequences belonging to Bracoviriform viruses and, for the first time, experimentally validated the presence of bracoviral genetic fragments in the CPB genome. Our work represents the first attempt to discover the viral genetic material in CPB samples, and we hope that further studies will help to identify new viruses to extend the arsenal of biopesticides against CPB.

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“…Almost a third of crop diseases could be linked to plant-infecting viruses [ 84 ]. To improve crop resistance against specific known viruses, the idea of specifically targeting viral genomes mimicking the bacterial CRISPR/Cas9 system during the infection in plants rapidly grew ( Figure 4 ).…”

Section: Crispr/cas-system As a Tool To Target Virusesmentioning

confidence: 99%

Not Only Editing: A Cas-Cade of CRISPR/Cas-Based Tools for Functional Genomics in Plants and Animals

Devillars,

Magon,

Pirrello

et al. 2024

IJMS

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The advent of CRISPR/Cas9 technology has revolutionized genome editing, enabling the attainment of once-unimaginable goals. CRISPR/Cas’s groundbreaking attributes lie in its simplicity, versatility, universality, and independence from customized DNA-protein systems, erasing the need for specialized expertise and broadening its scope of applications. It is therefore more and more used for genome modification including the generation of mutants. Beyond such editing scopes, the recent development of novel or modified Cas-based systems has spawned an array of additional biotechnological tools, empowering both fundamental and applied research. Precisely targeting DNA or RNA sequences, the CRISPR/Cas system has been harnessed in fields as diverse as gene regulation, deepening insights into gene expression, epigenetic changes, genome spatial organization, and chromatin dynamics. Furthermore, it aids in genome imaging and sequencing, as well as effective identification and countering of viral pathogens in plants and animals. All in all, the non-editing aspect of CRISPR/Cas exhibits tremendous potential across diverse domains, including diagnostics, biotechnology, and fundamental research. This article reviews and critically evaluates the primary CRISPR/Cas-based tools developed for plants and animals, underlining their transformative impact.

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Mechanisms of Microbial Plant Protection and Control of Plant Viruses

Cited by 29 publications

References 203 publications

Insight into the soil bacterial community succession of Nicotiana benthamiana in response to Tobacco mosaic virus

Insight into the soil bacterial community succession of Nicotiana benthamiana in response to Tobacco mosaic virus

The Metagenomic Analysis of Viral Diversity in Colorado Potato Beetle Public NGS Data

Not Only Editing: A Cas-Cade of CRISPR/Cas-Based Tools for Functional Genomics in Plants and Animals

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