Effect of vitamin C and E on oxidative stress and antioxidant system in the salivary glands of STZ-induced diabetic rats

Ibuki, Flavia Kazue; Bergamaschi, Cássia Toledo; Pedrosa, Marlus da Silva; Nogueira, Fernando

doi:10.1016/j.archoralbio.2020.104765

Cited by 28 publications

(13 citation statements)

References 88 publications

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“…This evidence suggests that antioxidants are a promising additional treatment for DM. 15 Deconte et al 11 showed an increase in the MDA content in the PA glands of diabetic rats 30 days after STZ induction. In addition, Zalewska et al 13 demonstrated that the antioxidant defense of the PA glands is deficient throughout the duration of experimentally induced diabetes and suggested that changes in MDA depend on the type of salivary glands studied in rats.…”

Section: Discussionmentioning

confidence: 97%

“…14 Such evidence suggests that antioxidants are a promising additional treatment for DM. 15 Epidemiological data have demonstrated that chamomile consumption is beneficial in DM because of its antioxidant effect and biological properties. 16 In addition, previous studies have shown that treatment with chamomile tea or chamomile tea components led to a reduction in glycemia.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant effect of chamomile tea on the salivary glands of streptozotocin-induced diabetic rats

et al. 2022

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This study aimed to analyze oxidative stress and the activity of antioxidant enzymes in the salivary glands of streptozotocin (STZ)-induced diabetic rats with ad libitum consumption of chamomile tea in substitution of water for 21 days. Rats were divided in two control groups (untreated control and treated control) and two diabetic groups (untreated diabetic and treated diabetic). Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activities, total antioxidant status (TAS), and malondialdehyde (MDA) concentrations were determined. The chemical composition of the chamomile essential oil revealed 39 compounds, accounting for 93.5% of the total oils. The polyphenolic profile of the tea showed the presence of apigenin, luteolin, umbelliferone, and esculetin. SOD, GPx, CAT, and TAS levels were lower in the parotid (PA) diabetic glands, but treatment increased their concentration in both the submandibular (SM) and PA diabetic salivary glands. Increased MDA levels were observed in the PA diabetic glands, which were decreased by the consumption of chamomile tea with a reduction in hyperglycemia compared to that in untreated diabetic rats. However, the SM diabetic glands showed no difference in the MDA content. The consumption of chamomile tea prevented oxidative stress in the PA glands of diabetic rats, exhibiting hypoglycemic and antioxidant effects. Thus, chamomile tea could be a potential candidate for preventing oral complications in diabetes mellitus.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Antioxidant effect of chamomile tea on the salivary glands of streptozotocin-induced diabetic rats

et al. 2022

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“…The effectiveness of STZ-induced beta-cell mass destruction expressed as a high glucose concentration in serum is not in question, which was also shown in these studies. There are numerous research studies where blood glucose concentration was permanently elevated with the administration of STZ in rodent models [ 35 , 49 , 50 , 51 ] and on rhesus monkeys [ 52 , 53 ]. Nevertheless, an appropriate technique for the induction of over 90% beta-cell mass destruction by STZ administration is highly needed.…”

Section: Discussionmentioning

confidence: 99%

Streptozotocin-Induced Diabetes in a Mouse Model (BALB/c) Is Not an Effective Model for Research on Transplantation Procedures in the Treatment of Type 1 Diabetes

Wszoła¹,

Klak²,

Kosowska

et al. 2021

Biomedicines

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Type 1 diabetes (T1D) is characterized by the destruction of over 90% of the β-cells. C-peptide is a parameter for evaluating T1D. Streptozotocin (STZ) is a standard method of inducing diabetes in animals. Eight protocols describe the administration of STZ in mice; C-peptide levels are not taken into account. The aim of the study is to determine whether the STZ protocol for the induction of beta-cell mass destruction allows for the development of a stable in vivo mouse model for research into new transplant procedures in the treatment of type 1 diabetes. Materials and methods: Forty BALB/c mice were used. The animals were divided into nine groups according to the STZ dose and a control group. The STZ doses were between 140 and 400 mg/kg of body weight. C-peptide was taken before and 2, 7, 9, 12, 14, and 21 days after STZ. Immunohistochemistry was performed. The area of the islet and insulin-/glucagon-expressing tissues was calculated. Results: Mice who received 140, 160, 2 × 100, 200, and 250 mg of STZ did not show changes in mean fasting C-peptide in comparison to the control group and to day 0. All animals with doses of 300 and 400 mg of STZ died during the experiment. The area of the islets did not show any differences between the control and STZ-treated mice in groups below 300 mg. The reduction of insulin-positive areas in STZ mice did not exceed 50%. Conclusions: Streptozotocin is not an appropriate method of inducing a diabetes model for further research on transplantation treatments of type 1 diabetes, having caused the destruction of more than 90% of the β-cell mass in BALB/c mice.

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“…The consequence of reduced saliva secretion may be subjective dryness of the oral mucosa (xerostomia) and an increased incidence of dental caries, periodontal diseases, and fungal infections [ 14 , 67 ]. Considering that antioxidant supplementation improves the secretory function of salivary glands in various systemic diseases [ 19 , 68 – 70 ], the administration of free radical scavengers may be considered in stroke patients. Nevertheless, further studies are needed.…”

Section: Discussionmentioning

confidence: 99%

Salivary Gland Dysfunction in Stroke Patients Is Associated with Increased Protein Glycoxidation and Nitrosative Stress

Maciejczyk

Gerreth²,

Hojan

et al. 2020

Oxidative Medicine and Cellular Longevity

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Stroke is one of the leading causes of disability and death worldwide. Despite intensive medical care, many of the complaints directly threatening the patient’s life marginalize their dental needs after the stroke. Recent studies indicate reduced saliva secretion in stroke patients in addition to the increased incidence of caries and periodontal disease. Since oxidative stress plays a vital role in the pathogenesis of salivary gland hypofunction and neurodegenerative disorders (including stroke), this is the first to evaluate the relationship between salivary gland activity and protein glycoxidation and nitrosative damage. The content of glycation and protein oxidation products and nitrosative stress was assessed in nonstimulated (NWS) and stimulated (SWS) whole saliva of stroke patients with normal salivary secretion and hyposalivation (reduced saliva production). The study included 30 patients in the stroke’s subacute phase and 30 healthy controls matched by age and sex. We have shown that stroke patients with hyposalivation show increased contents of protein glycation (↑Amadori products and ↑advanced glycation end products), glycoxidation (↑dityrosine), and nitration (↑nitrotyrosine) products compared to stroke cases with normal salivary secretion and control group. Interestingly, higher oxidative/nitrosative stress was found in NWS, which strongly correlates with salivary flow rate, total protein content, and salivary amylase activity. Such relationships were not observed in the control group. Summarizing, oxidative and nitrosative stress may be one of the mechanisms responsible for the impairment of saliva secretion in stroke patients. However, extraglandular sources of salivary oxidative stress in stroke patients cannot be excluded. Further studies to assess salivary gland hypofunction in stroke cases are necessary.

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Effect of vitamin C and E on oxidative stress and antioxidant system in the salivary glands of STZ-induced diabetic rats

Cited by 28 publications

References 88 publications

Antioxidant effect of chamomile tea on the salivary glands of streptozotocin-induced diabetic rats

Antioxidant effect of chamomile tea on the salivary glands of streptozotocin-induced diabetic rats

Streptozotocin-Induced Diabetes in a Mouse Model (BALB/c) Is Not an Effective Model for Research on Transplantation Procedures in the Treatment of Type 1 Diabetes

Salivary Gland Dysfunction in Stroke Patients Is Associated with Increased Protein Glycoxidation and Nitrosative Stress

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