Effect of vitamin E and selenium supplementation on haematology, blood chemistry and thyroid hormones in male buffalo (<i>Bubalus bubalis</i>) calves

Abstract

An experiment was conducted on 20 male buffalo calves (average body weight 75.30 ± 2.20 kg) to elucidate the effect of supplemental vitamin E and/or selenium (Se) on their haematology, blood metabolic profi le, serum enzymes and thyroid hormones status. Group I served as control (without any supplementation), groups II, III and IV were supplemented with 0.3 mg Se, 300 IU of DL-α-tocopheryl acetate, and both 300 IU DL-α-tocopheryl acetate and 0.3 mg Se, respectively. Animals were fed on wheat straw and concentrate mixture to meet their nutrient requirements. Blood was collected at zero day and subsequently at 45, 90, 135 and 180 days and analysed for haematological parameters, blood chemistry and level of thyroid hormones. Results revealed no signifi cant difference (P>0.05) in haematological parameters (haemoglobin, packed cell volume, red blood cell, white blood cell) due to the supplementation of vitamin E ,Se or both vitamin E + Se in buffalo calves. Similarly, the concentration of various blood biochemical components (glucose, total protein, albumin, globulin, urea, creatinine, total cholesterol, and triglycerides) except high density lipid (HDL) cholesterol was statistically similar in 4 groups. Results revealed signifi cantly (P<0.01) higher concentration (mg/dl) of HDL cholesterol in buffalo calves given Se and Se + vitamin E as compared to control and vitamin E supplemented groups. There was no signifi cant difference in serum enzymes (serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, alkaline phosphatase, creatine kinase and lactate dehydrogenase) activities due to different treatments. Level of triiodothyronine was signifi cantly (P<0.01) higher in buffalo calves supplemented with selenium, and vitamin E + Se, as compared to non-supplemented control group; whereas concentration of thyroxine (nmol/l) was statistically similar in 4 groups, without showing * This research work was carried out under AP Cess Fund Ad Hoc scheme supported by Indian Council of Agricultural Research, Krishi Bhawan, New Delhi (India) 1 Corresponding author: e-mail: rsd@ivri.up.nic.in Animal and Feed Sciences, 18, 2009, 241-256 242 VITAMIN E AND SE SUPPLEMENT -THYROID HORMONES IN CALVES any effect of different treatments. It may be concluded that supplementation of Se and vitamin E in the diet of buffalo calves had no benefi cial effect on blood haematology and blood chemistry except an increase in the serum levels of high density lipid cholesterol and triiodothyronine as compared to non-supplemented control buffalo calves. Journal of

Effect of different selenium sources (Selenium yeast and Sodium selenite) on haematology, blood chemistry and thyroid hormones in male goats (Capra hircus)

“…The mean haemoglobin (Hb) values (8.9 to 9.3 g/dl) and PCV (27.9 to 28.5 %) values were in normal range and were found to be comparable (P>0.05) in all the three groups. Similar findings was observed by Shinde et al (2009) in buffalo calves who observed that supplementation of selenium and vitamin E had no effect on the haematological parameters viz., Hb and PCV. Contrary to the above findings, Qureshi et al (2001) reported significantly (P<0.05) higher haemoglobin concentration and PCV values in buffaloes supplemented with selenium.…”

“…Similarly Arthur et al (1988) did not observe any effect of supplementation of Se on the serum total protein and albumin levels of Friesian steers. Similarly Shinde et al (2009) observed that supplementation of either Se (0.3 ppm) or vitamin E (300 IU) or both (300 IU vitamin E and 0.3 ppm Se) in the diet had no effect on serum albumin, globulin and albumin: globulin ratio (P>0.05) in buffalo calves. The mean values of total cholesterol, although numerically higher in Se supplemented groups (108.4 mg/dl) as compared to control (103.4 mg/dl), but did not reveal any statistical (P>0.05) differences.…”

“…Arthur et al (1988) also reported that the peripheral concentration of T 3 was reduced and that of T 4 was increased in calves that were fed a synthetic diet that was deficient in Se as compared to the calves that were fed the same diet supplemented with Se. Similarly, Shinde et al (2009) Kumar et al (2009) observed that supplementation of 0.15 ppm Se both as organic and inorganic form to lambs for 90 days had no effect on serum concentration of T 3 , T 4 and T 4 : T 3 ratio. Increased T 3 in the blood due to nutritional factor is indicative of the better metabolic balance (Blum et al, 1980) and better enzyme activity thus to regulate the thyroid hormone homeostasis efficiently (Chadio et al, 2006).…”

“…Non-significant (P>0.05) variation in serum urea (30.6 to 31.4 mg/dl) and creatinine (0.90 to 0.92 mg/dl) in 3 groups of goats were similar to the findings of Mudgal et al (2012) who reported that supplementation of Se in the diet of buffalo calves had no effect on their serum urea and creatinine level. Similarly, Shinde et al (2009) observed supplementation of Se (0.3 ppm) or vitamin E (300 IU) or both in the diet of buffalo calves had no effect on their serum urea and creatinine level and concluded about its no effect on nitrogen metabolism of goats.…”

Blood biochemical indicators and concentration of thyroid hormones in heavily pregnant and lactating ewes depending on selenium supplementation

“…Increased T 3 concentration can be explained by increased activity of enzyme type 4 deiodinase in the conversion of thyroxine T 4 into the more active triiodothyronine T 3 and is in positive correlation with energy and nitrogen balance ration. Shinde et al (2009) showed significantly higher activity of T 3 hormone in the blood of buffalo calves that were fed with the addition of Se (sodium selenite), compared to those without added Se. Similarly Nayyar et al (2003) also reported that buffalo heifers that were supplemented with Se had significantly (P < 0.05) higher T 3 concentrations compared to unsupplemented control animals.…”