Effect of vitrification and thawing on human oocyte ATP concentration

Effect of vitrification on mitochondrial membrane potential in human metaphase II oocytes

“…Chen SU et al reviewed eight published studies mainly in mouse oocytes, and seven of them revealed that oocytes collected immediately after thawing displayed severe disorganization or disappearance of spindles, but recovered to diverse degrees after incubation for 1-3 h at 37°C [28]. Manipalviratn S et al evaluated the effect of vitrification and thawing on the intra-oocyte ATP concentration compared to non-vitrified oocytes, and demonstrated that vitrification and thawing procedure has a negative effect on intra-oocyte ATP level, but 3 h incubation after post-thaw, the intra-oocyte ATP level returned to a level significantly higher than that of immediate post-thawed oocytes but still lower than fresh oocytes [29]. From the above studies and our experiment, the recovery time of meiotic spindle reorganization, ATP and ΔΨm are consistent, approximately 3-4 h to recover after thawing.…”

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

“…Moreover, developmental failure in the preimplantation embryo may result from an abnormal distribution of mitochondria in the oolemma [52]. Vitrification has been reported to compromise mitochondrial function and reduce ATP content in human [84] and bovine [85] oocytes, which might contribute to poor oocyte development after cryopreservation [85]. The intracellular distribution of mitochondria is dependent on microtubules [86], which is important for redistribution of ATP and allows increased levels of ATP to be produced in different intracellular areas during periods of high energy requirements [86,87].…”

RNA-Seq transcriptome profiling of mouse oocytes after in vitro maturation and/or vitrification

“…All of these genes were up-regulated and related to NADH dehydrogenase activity, and are in accordance with previous results showing that the ratio of FAD/NAD(P)H is significantly higher in vitrified human oocytes 10 . An increased ratio of FAD/NAD(P)H suggests a decreased ATP level in vitrified human oocytes 9,11 , which can significantly affect spindle integrity and chromosome alignment 10, 12 . In vitro matured porcine oocytes obtained from the GV vitrification group exhibited higher abnormal configurations of spindles and chromosomes than oocytes from the MII vitrification group 31 .…”

“…An increased ratio of FAD/NAD(P)H suggests a decreased ATP level in vitrified human metaphase II (MII, the second stage in meiosis II after prophase II) stage oocytes 10,11 , which significantly affects spindle integrity and chromosome alignment 12 . There are also other factors that may influence the cryopreservation efficiency of oocytes, in particular the development stage of the oocytes.…”