Effect of vitrification on mitochondrial membrane potential in human metaphase II oocytes

Han, Shubiao; Liu, Weiwei; Wang, Yaping; Huang, Gangliang

doi:10.1007/s10815-012-9848-1

Cited by 41 publications

(32 citation statements)

References 31 publications

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“…In the present study, a significant decrease in fluorescence intensity of oocytes subjected to vitrification was observed (Figure ). Our findings are consistent with results of studies on the oocytes of other species (Chen et al., ; Lei et al., ; Nazmara et al., ; Zander‐Fox et al., ). Zander‐Fox et al.…”

Section: Discussionsupporting

confidence: 93%

“…() suggested that the decrease in mitochondrial activity may be due to osmotic stress within the oocyte caused by exposure to high level of cryoprotectants which are used in vitrification procedure to prevent ice crystal formation. Other authors suggested that non‐physiological temperatures, phase transition, pH instability and osmotic pressure change during dehydration–rehydration might also be factors affecting the function or number of mitochondria (Chen et al., ; Lei et al., ; Nazmara et al., ; Shi et al., ). Taking together, we hypothesize that the fluorescence intensity of MitoTracker ® Red CMXRos affected by vitrification may reflect a decreasing meiotic and developmental competence of oocyte.…”

Section: Discussionmentioning

confidence: 99%

“…In different mammalian species, changes in mitochondrial traits after vitrification have been shown. Most authors highlights a decrease in activity of mitochondria upon vitrification (Chen, Han, Liu, Wang, & Huang, ; Lei et al., ; Nazmara, Salehnia, & HosseinKhani, ; Shi et al., ; Zander‐Fox, Cashman, & Lane, ), but changes in distribution (Lei et al., ) and morphology of mitochondria were also observed (Turathum et al., ) in mouse and dog oocytes, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Mitochondrial characteristics in oocytes of the domestic cat (Felis catus) after in vitro maturation and vitrification

Sowińska

Müller

Niżański

et al. 2017

Reprod Domestic Animals

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The objective of this study was to evaluate mitochondria in immature and in vitro-matured domestic cat oocytes and to assess for the first time the effect of vitrification on mitochondrial traits. Mitochondrial distribution and aggregation were assessed using confocal microscopy after staining with the fluorescent dye-MitoTracker Red CMXRos. Only cells at the germinal vesicle and the metaphase II stages of nuclear development, representing immature and mature oocytes, respectively, were included in our study. Our study shows that 80% of immature and 100% of mature oocytes exhibit a peripheral pattern of mitochondria distribution, indicating that, in contrast to the situation in other species, the mitochondria of cat oocytes are not dispersed throughout the cell after in vitro maturation but instead maintain a strong affinity for the oocyte periphery near the membrane. However, a loss of aggregation was observed during in vitro maturation-78% of immature oocytes showed homogeneous granulation versus only 18% of mature oocytes (p < .001). The increased intensity of MitoTracker Red CMXRos staining after in vitro maturation (p < .05) may be tentatively attributed to an increase in mitochondrial activity but could likewise reflect a concomitant appearance of sulphhydryl groups in cytoplasm (known to be targeted by the dye). Mitochondrial distribution did not change upon vitrification; however, dye intensity decreased (p < .05) and mitochondrial aggregation was intensified in both immature and mature vitrified cat oocytes.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mitochondrial characteristics in oocytes of the domestic cat (Felis catus) after in vitro maturation and vitrification

Sowińska

Müller

Niżański

et al. 2017

Reprod Domestic Animals

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“…Lei et al [17,18] suggested that vitrification significantly affects mitochondrial function and caused the failure of embryo development after fertilization in both mouse oocytes and human IVM oocytes. Jones et al [15] and Chen et al [5] used programmed freezing method and vitrification method, respectively, to cryopreserve human MII-stage oocytes; both groups reported that the irreversible loss of high DWm in thawed oocytes may be associated with their poor developmental ability after in vitro fertilization. Zander-Fox et al [50] showed that mouse oocytes vitrification altered mitochondrial distribution and membrane potential.…”

Section: Discussionmentioning

confidence: 99%

Changes in mitochondrial function in porcine vitrified MII-stage oocytes and their impacts on apoptosis and developmental ability

et al. 2015

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“…The information available on the neonatal outcome and the long-term follow-up of children conceived after oocyte cryopreservation is limited (Chian et al, 2008(Chian et al, , 2014Noyes et al, 2009;Wennerholm et al, 2009;Cobo et al, 2014) and only small studies have specifically addressed the safety of oocyte vitrification (Coticchio et al, 2009;Bonetti et al, 2011;Chen et al, 2012;Forman et al, 2012;Khalili et al, 2012;Monzo et al, 2012;Dominguez et al, 2013;Gugliemo et al, 2014;Konc et al, 2014;Nohales Có rcoles et al, 2014;Palmerini et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Chromosomal meiotic segregation, embryonic developmental kinetics and DNA (hydroxy)methylation analysis consolidate the safety of human oocyte vitrification

Munck

Petrussa

Verheyen³

et al. 2015

MHR: Basic Science of Reproductive Medicine

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Oocyte vitrification has been introduced into clinical settings without extensive pre-clinical safety testing. In this study, we analysed major safetyaspects of human oocyte vitrification in a high security closed system: (i) chromosomal meiotic segregation, (ii) embryonic developmental kinetics and (iii) DNA (hydroxy)methylation status. Fresh and vitrified sibling oocytes from young donors after intracytoplasmic sperm injection (ICSI) were compared in three different assays. Firstly, the chromosomal constitution of the fertilized zygotes was deduced from array comparative genomic hybridization results obtained from both polar bodies biopsied at Day 1. Secondly, embryo development up to Day 3 was analysed by time-lapse imaging. Ten specific time points, six morphokinetic time intervals and the average cell number on Day 3 were recorded. Thirdly, global DNA methylation and hydroxymethylation patterns were analysed by immunostaining on Day 3 embryos. The nuclear fluorescence intensity was measured by Volocity imaging software. Comprehensive chromosomal screening of the polar bodies demonstrated that at least half of the zygotes obtained after ICSI of fresh and vitrified oocytes were euploid. Time-lapse analysis showed that there was no significant difference in cleavage timings, the predictive morphokinetic time intervals nor the average cell number between embryos developed from fresh and vitrified oocytes. Finally, global DNA (hydroxy)methylation patterns were not significantly different between Day 3 embryos obtained from fresh and from vitrified oocytes. Our data further consolidate the safety of the oocyte vitrification technique. Nevertheless, additional testing in young and older sub-fertile/infertile patients and sound follow-up studies of children born after oocyte cryopreservation remain mandatory.

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Effect of vitrification on mitochondrial membrane potential in human metaphase II oocytes

Abstract: Objective The aim of this study was to evaluate the impact of vitrification on mitochondrial membrane potential (ΔΨm) in human metaphase II (MII) oocytes, and the changes of ΔΨm on thawed MII oocytes.

Cited by 41 publications

References 31 publications

Mitochondrial characteristics in oocytes of the domestic cat (Felis catus) after in vitro maturation and vitrification

Mitochondrial characteristics in oocytes of the domestic cat (Felis catus) after in vitro maturation and vitrification

Changes in mitochondrial function in porcine vitrified MII-stage oocytes and their impacts on apoptosis and developmental ability

Chromosomal meiotic segregation, embryonic developmental kinetics and DNA (hydroxy)methylation analysis consolidate the safety of human oocyte vitrification

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