Studying the effects of hybrid reinforcement made of nano-zirconium oxide and chopped glass fibers for poly methyl methacrylate (PMMA) denture bases, nano- ZrO2 (n ZrO2) at grain sizes (46.7 nm) which measured using Atomic Force Microscopy (AFM) and different ratios on the mechanical characteristics was the aim of this study .To make the pure sample, a 2:1 powder to liquid ratio of typical acrylic resin was used. Hybrid composites with varying concentrations of nano-ZrO2 (nZrO2) and glass fiber (GFs) (2.5%nZrO2+2.5% GFs), (3.5%nZrO2+1.5%GFs), (1.5%nZrO2+3.5% GFs) were introduced. Mechanical tests as tensile strength, flexural strength, impact strength, shore D hardness are used to learn about the properties of manufactured composites. Antibacterial testing, water absorption, density testing, and thermal conductivity these indicated that composites may be effectively turned into filler material. In addition to X-ray diffraction (XRD), Scanning Electron Microscopy (SEM). The result show increased the mechanical properties of the PMMA denture base material. The tensile strength flexural strength, and impact strength increase for all hybrid ratio and reach maximum value at the concentration (2.5%nZrO2+2.5% GFs) compared to unreinforced PMMA.While hardness increase and reach maximum value at the concentration (3.5%nZrO2+1.5% GFs). In addition, thermal conductivity and density, the maximum value at concentration (1.5%nZrO2+3.5% GFs), as well as water absorption decrease as ratio of nZrO2 increase this shoed in concentration (3.5wt. % nZrO2 +1.5wt. %, GFs).