Effect of WOW process parameters on morphology and burst release of FITC-dextran loaded PLGA microspheres

Mao, Shirui; Xu, Jing; Cai, Cuifang; Germershaus, Oliver; Schaper, Andreas; Kissel, Thomas

doi:10.1016/j.ijpharm.2006.10.036

Cited by 246 publications

(191 citation statements)

References 27 publications

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“…This stabilization effect became more dominant at higher stabilizer concentrations, which decrease the size and PDI of NPs. These results are in agreement with those obtained by Mao et al, who reported that increasing PVA concentration caused a marked decrease in the particle size and PDI of PLGA microspheres loaded with fluorescein isothiocyanatelabeled dextrans [34]. The stabilizer concentration also had an inverse relationship with the zeta potential, which doi: 10.7243/2050-120X-2-7…”

Section: Effect Of Stabilizer and Its Concentrationsupporting

confidence: 92%

Natural bioadhesive biodegradable nanoparticles-based topical ophthalmic formulations for sustained celecoxib release: in vitro study

Ibrahim¹,

Abd-Elgawad²,

Soliman³

et al. 2013

J Pharm Technol Drug Res

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Background: Our goal was to prepare and evaluate topical ophthalmic formulations containing optimized celecoxib-loaded bioadhesive cationic chitosan or anionic alginate nanoparticles for sustained release of celecoxib. Methods: Nanoparticles were prepared using a spontaneous emulsification solvent diffusion method. Different concentrations of polymers, emulsifier and stabilizers were used to optimize our formulations. The formulation that gave the lowest particle size and polydispersity index and the highest zeta potential was selected and subjected to further studies. The optimized celecoxibloaded NPs were characterized with regard to their particle size, polydispersity index, zeta potential, morphology and drug content. Celecoxib-loaded NPs were incorporated in topical ophthalmic dosage forms including eye drops, temperature-triggered in situ gelling system and preformed gel. All formulations were then characterized regarding their pH, viscosity, uniformity of drug content, in vitro release study and in vitro cytotoxicity. Results: Results of the optimization studies of both celecoxib-loaded chitosan and alginate nanoparticles respectively, are: particle size of 113.33 ± 4.08 nm and 154.67 ± 5.06 nm; zeta potential of +36.92 ± 3.38 mV and -36.5 ± 4.7 mV; and encapsulation efficiencies of 89.88 ± 4.17% and 75.38 ± 2.98%. Transmission electron microscopic analysis revealed that all nanoparticles have distinct spherical shapes comprising of a solid dense core covered with evenly distributed coat. oreover, all formulations possessed pH and viscosity values that are compatible with the eye and have uniform drug contents that complied with the US Pharmacopeial (USP) official requirement. In vitro release data of ophthalmic formulations showed a sustained release without any burst effect and the formulations followed a Higuchi non-Fickian diffusion mechanism. The results of in vitro cell toxicity revealed that all the prepared formulations are non-toxic, as the percentage cell viability ranged from 89.9 to 97.7%. Conclusions: These formulations provide a great deal of flexibility to the formulation scientist whereby the sizes and zeta potentials of the formulations can be tuned to suit the need using scalable and robust methodologies. This could thus serve as a potential drug delivery system for both the anterior and posterior eye diseases.

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Section: Effect Of Stabilizer and Its Concentrationsupporting

confidence: 92%

Natural bioadhesive biodegradable nanoparticles-based topical ophthalmic formulations for sustained celecoxib release: in vitro study

Ibrahim¹,

Abd-Elgawad²,

Soliman³

et al. 2013

J Pharm Technol Drug Res

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“…PLGA has gained much attention and research focus in controlled release of therapeutic proteins (Estey et al, 2006). The most common emulsification technique for protein microencapsulation in PLGA microspheres is the W/O/W emulsion method (Mao et al, 2007;Wu et al, 2008). Initial burst is one of the major challenges in protein-encapsulated microparticle systems (Yeo & Park, 2004).…”

Section: Introductionmentioning

confidence: 99%

A one-step modified method to reduce the burst initial release from PLGA microspheres

Zheng

Liang

2010

Drug Delivery

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“…[24][25][26][27] PLGA was used as the core of the drug carrier, Fe 3 O 4 was embedded in the layer of PLGA, and cRGD was coated on the surface of the PLGA-Fe 3 O 4 layer. A schematic representation of the Fe 3 O 4 -PLGA-cRGD NPs is shown in Figure 1.…”

Section: Preparation and Physical And Chemical Characterization Of Thmentioning

confidence: 99%

Fe<sub>3</sub>O<sub>4</sub>-based PLGA nanoparticles as MR contrast agents for the detection of thrombosis

Liu¹,

Xu²,

Zhou³

et al. 2017

IJN

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Thrombotic disease is a great threat to human health, and early detection is particularly important. Magnetic resonance (MR) molecular imaging provides noninvasive imaging with the potential for early disease diagnosis. In this study, we developed Fe 3 O 4 -based poly(lactic- co -glycolic acid) (PLGA) nanoparticles (NPs) surface-modified with a cyclic Arg-Gly-Asp (cRGD) peptide as an MR contrast agent for the detection of thrombosis. The physical and chemical characteristics, biological toxicity, ability to target thrombi, and biodistribution of the NPs were studied. The Fe 3 O 4 -PLGA-cRGD NPs were constructed successfully, and hematologic and pathologic assays indicated no in vivo toxicity of the NPs. In a rat model of FeCl 3 -induced abdominal aorta thrombosis, the NPs readily and selectively accumulated on the surface of the thrombosis and under vascular endothelial cells ex vivo and in vivo. In the in vivo experiment, the biodistribution of the NPs suggested that the NPs might be internalized by the macrophages of the reticuloendothelial system in the liver and the spleen. The T2 signal decreased at the mural thrombus 10 min after injection and then gradually increased until 50 min. These results suggest that the NPs are suitable for in vivo molecular imaging of thrombosis under high shear stress conditions and represent a very promising MR contrast agent for sensitive and specific detection of thrombosis.

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Effect of WOW process parameters on morphology and burst release of FITC-dextran loaded PLGA microspheres

Cited by 246 publications

References 27 publications

Natural bioadhesive biodegradable nanoparticles-based topical ophthalmic formulations for sustained celecoxib release: in vitro study

Natural bioadhesive biodegradable nanoparticles-based topical ophthalmic formulations for sustained celecoxib release: in vitro study

A one-step modified method to reduce the burst initial release from PLGA microspheres

Fe<sub>3</sub>O<sub>4</sub>-based PLGA nanoparticles as MR contrast agents for the detection of thrombosis

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