Effect of Y-24180, an antagonist at platelet-activating factor (PAF) receptors, on IgE-mediated cutaneous reactions in mice

Abstract

These results indicate that PAF may be involved in the induction of biphasic cutaneous reactions mediated by IgE, and Y-24180 is more effective compared with WEB2086 in this model. It is possible that the difference in the effectiveness between Y-24180 and WEB2086 depends on the persistence of those activities.

Inhibitory Effect of Dietary Carotenoids on Dinitrofluorobenzene-Induced Contact Hypersensitivity in Mice

“…1) Dinitrofluorobenzene (DNFB) has been used to examine the effect of certain compounds on contact hypersensitivity in mice by measuring the biphasic increase in ear thickness of the animals, regarded as ear swelling, brought on by a DNFB challenge. 2,3) The first phase of ear swelling appears immediately and peaks about 6 h after the challenge. The first response is not induced in mast cell-deficient WBB6F1-W/W v mice and is inhibited by the antihistamine antibody, showing that the response depends on the activation of mast cells.…”

Histamine Release Inhibitory Activity of Piper nigrum Leaf

“…2,4-Dinitrofluorobenzene (DNFB) has been shown to induce triphasic increase in ear thickness of mouse by the challenge after a passive sensitization with monoclonal antidinitrophenyl (DNP) IgE antibody. 8,9) This model serves as an animal model for type I allergy. 8,9) In addition, we used picryl chloride (PC)-induced contact dermatitis in mice as an animal model for type IV allergy.…”

“…8,9) This model serves as an animal model for type I allergy. 8,9) In addition, we used picryl chloride (PC)-induced contact dermatitis in mice as an animal model for type IV allergy.10) Inhibitory effects of Piper nigrum leaf extract, on histamine release were also examined by in vitro assay using compound 48/80-induced histamine release from rat peritoneal mast cells. …”

“…8,9) This model serves as an animal model for type I allergy. 8,9) In addition, we used picryl chloride (PC)-induced contact dermatitis in mice as an animal model for type IV allergy.…”

“…IgE-Mediated Cutaneous Reaction in Mice IgE-mediated cutaneous reaction was induced according to the methods of Yamaguchi et al 8) and Tahara et al 9) with minor modifications. Female BALB/c strain mice weighing 14-19 g (nϭ10 to 11 per group) were passively sensitized with intra- venous injection of 10 mg of monoclonal anti-DNP IgE antibody dissolved in 0.5 ml of saline.…”