Effect of Zofenopril on regeneration of sciatic nerve crush injury in a rat model

Abstract

BackgroundZofenopril is an antioxidant agent which has been shown to have beneficial effects in hypertension and heart failure. The aim of this study was to test the effects of Zofenopril on nerve regeneration and scarring in a rat model of peripheral nerve crush injury.MethodsTwenty-one adult Sprague-Dawley rats underwent a surgical procedure involving right sciatic nerve crush injury. 15 mg/kg Zofenopril was administered orally to seven rats in group Z for seven days. Seven rats in group S received saline orally for seven days. Seven rats in the control group C received no drug after crush injury. Fourteenth and 42nd days after injury, functional and electromyography assessments of nerves were performed. Functional recovery was analyzed using a walking track assessment, and quantified using the sciatic functional index (SFI). After these evaluations, all rats were sacrificed and microscopic evaluations were performed.ResultsThe Sciatic functional Index (SFI) in group Z on 14th day is different significantly from group S and group C (p = 0.037). But on 42nd day there was no difference between groups (p = 0.278). The statistical analyses of electromyelographic (EMG) studies showed that the latency in group Z is significantly different from group S (p = 0.006) and group C (p = 0.045). But on 42nd day there was no difference between groups like SFI (p = 0.147). The amplitude was evaluated better in group Z than others (p < 0.05). In microscopic evaluation, we observed the highest number of nerve regeneration in the group Z and the lowest in the group C. But it was not significant statistically.ConclusionOur results demonstrate that Zofenopril promotes the regeneration of peripheral nerve injuries in rat models.

Antinociceptive and Anti-Inflammatory Effects of Nypa fruticans Wurmb by Suppressing TRPV1 in the Sciatic Neuropathies

“…The rat underwent general anesthesia with a 2 cm incision of the skin on its right posterior femur, minimizing the damage to the surrounding tissues. Hemostatic forceps were used to crush the exposed sciatic nerve for 30 s according to the modified procedure described by Kalender, A [21]. The wound was then sutured, and the skin was sterilized to prevent infection.…”

“…The wound was then sutured, and the skin was sterilized to prevent infection. The wound was stabilized by putting the rat in a cage for experimental animals [21].…”

Fish oil protects the peripheral and central nervous systems against cisplatin-induced neurotoxicity

“…Neurophysiological analysis was performed according to the method of Kalender et al 14 Briefly, for stimulation, the sciatic nerve was stimulated percutaneously with a supramaximal stimulus for determining compound muscle action potentials (CMAPs) with monopolar needle electrodes. Electrical stimulation was a square pulse with a frequency of 1 Hz and duration of 0.2 ms.…”

Acute Phase Effect of Memantine Hydrochloride in Experimental Peripheral Nerve Injury

“…[4][5][6][7]18 Experimental peripheral nerve studies were generally investigated for long-term effects (4-8 weeks). 7,19,20 However, we medicated the animals for 7 days and sacrificed them on the eighth day to observe the short-term effects.…”