Effective Dispensing Methods for Loading Drugs Only to the Tip of DNA Microneedles

Abstract

Here, we propose a novel and simple method to efficiently capture the diffusion of fluorescein isothiocyanate (FITC)-dextran from a biocompatible substance and load the drug only to the tip of DNA microneedles. A dispensing and suction method was chosen to fabricate the designed microneedles with efficient amounts of FITC as the drug model. Importantly, the vacuum process, which could influence the capturing of FITC diffusion from the tip, was evaluated during the manufacturing process. In addition, the simulations were consistent with the experimental results and showed apparent diffusion. Moreover, dextrans of different molecular weights labeled with FITC were chosen to fabricate the tip of microneedles for demonstrating their applicability. Finally, a micro-jetting system with a micro-nozzle (diameter: 80 μm) was developed to achieve the accurate and rapid loading of small amounts of FITC using the anti-diffusion and micro-jetting methods. Our method not only uses a simple and fast manufacturing process, but also fabricates the tips of microneedles more efficiently with FITC compared with the existing methods. We believe that the proposed method is essential for the clinical applications of the microneedle drug delivery platform.

A Mobile Two‐Dimensional Ultrasound Focusing System for Personalized Healthcare Applications through a Dodecagonal Quasicrystal Patterned Planar Lens

“…Still, it is decelerated when the adjacent tissues are saturated with the target drug. [ 7,26,27 ] Therefore, the change in ε for the control and non‐focused group is small for the first 10 min of the ultrasound propagation. However, utilization of the MU2F system accelerated the dissipation of the dissolved and saturated drug to the adjacent tissue.…”

“…The microneedle adopted in this research was fabricated with a matrix material of salmon DNA and a rhodamine drug model. Details about the reason for the utilization of each material can be found in the previous paper, [7,26,27] and details about the fabrication of the microneedle patch can be found in the Experimental Section. As a result, the fabricated needle patch had a diameter of 5 mm and a needle length of 600 μm (Figure 5a).…”

Design and Evaluation of Dissolving Microneedles for Enhanced Dermal Delivery of Propranolol Hydrochloride

“…Meanwhile, the drug was concentrated in the tip of the needles via a two-step casting procedure to avoid drug waste during preparation and application. Having the therapeutics confined in the tip of the needles can minimize drug loss, control penetration depth, and ensure drug loading [ 51 ].…”

Biocompatible All-in-One Adhesive Needle-Free Cup Patch for Enhancing Transdermal Drug Delivery

“…SDNA with excellent biocompatibility and biodegradability has been used as a raw material for clinical treatment. This material has advantages that can help to repair skin and regenerate cartilage, which can also be used as a template for hosting various functional substances, such as proteins and drugs. ,− Therefore, in the present study, the biocompatible SDNA film was used to maintain the stability of drug loading in the adhesive cup patches. In addition, a gel containing moisture, as an experimental skin, was used to evaluate the performance of the microcup patches in delivering a drug.…”