Effective inhibition and eradication of pathogenic biofilms by titanium dioxide nanoparticles synthesized using <i>Carum copticum</i> extract

Altaf, Mohammad; Zeyad, Mohammad Tarique; Hashmi, Md Amiruddin; Manoharadas, Salim; Hussain, Shaik Althaf; Abuhasil, Mohammed Saeed Ali; Almuzaini, Mohammed Abdulaziz M.

doi:10.1039/d1ra02876f

Cited by 27 publications

(10 citation statements)

References 58 publications

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“…It was shown that the amount of biofilm mass decreased with an increasing concentration of nanoforms. Fatima et al [ 50 ] and Altaf et al [ 51 ] obtained similar results. Fatima et al [ 50 ] showed that exposure of oral bacterial strains ( Georgenia sp., Staphylococcus saprophyticus , and Rothia mucilaginosa ) to titanium dioxide nanoparticles (TiO 2 -NPs) significantly reduced biofilm formation in a concentration-dependent manner.…”

Section: Discussionsupporting

confidence: 54%

“…Fatima et al [ 50 ] showed that exposure of oral bacterial strains ( Georgenia sp., Staphylococcus saprophyticus , and Rothia mucilaginosa ) to titanium dioxide nanoparticles (TiO 2 -NPs) significantly reduced biofilm formation in a concentration-dependent manner. The presence of TiO 2 -NPs at concentrations of 8, 16, 32, and 64 μg/mL inhibited the development of the biofilm of P. aeruginosa PAO1, E. coli ATCC25922, and S. aureus MTCC3160 [ 51 ]. The results of the research presented by Maurer-Jones et al [ 52 ] showed that TiO2-NPs caused a slower growth of biofilm formed by Shewanella oneidensis .…”

Section: Discussionmentioning

confidence: 99%

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Benefits of Usage of Immobilized Silver Nanoparticles as Pseudomonas aeruginosa Antibiofilm Factors

Korzekwa

Kędziora

Stańczykiewicz

et al. 2021

IJMS

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The aim of this study was to assess the beneficial inhibitory effect of silver nanoparticles immobilized on SiO2 or TiO2 on biofilm formation by Pseudomonas aeruginosa—one of the most dangerous pathogens isolated from urine and bronchoalveolar lavage fluid of patients hospitalized in intensive care units. Pure and silver doped nanoparticles of SiO2 and TiO2 were prepared using a novel modified sol-gel method. Ten clinical strains of P. aeruginosa and the reference PAO1 strain were used. The minimal inhibitory concentration (MIC) was determined by the broth microdilution method. The minimal biofilm inhibitory concentration (MBIC) and biofilm formation were assessed by colorimetric assay. Bacterial enumeration was used to assess the viability of bacteria in the biofilm. Silver nanoparticles immobilized on the SiO2 and TiO2 indicated high antibacterial efficacy against P. aeruginosa planktonic and biofilm cultures. TiO2/Ag0 showed a better bactericidal effect than SiO2/Ag0. Our results indicate that the inorganic compounds (SiO2, TiO2) after nanotechnological modification may be successfully used as antibacterial agents against multidrug-resistant P. aeruginosa strains.

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Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Benefits of Usage of Immobilized Silver Nanoparticles as Pseudomonas aeruginosa Antibiofilm Factors

Korzekwa

Kędziora

Stańczykiewicz

et al. 2021

IJMS

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“…To evaluate the development of biofilms by E. cloacae strain MC9, as mentioned, the absorbance-based crystal violet (CV) technique was used as previously described. ,, In brief, bacterial strains were exposed to 25–200 μg mL –1 concentrations of Cr, Cd, Pb, and Ni.…”

Section: Experimental Sectionmentioning

confidence: 99%

Heavy Metals Induced Modulations in Growth, Physiology, Cellular Viability, and Biofilm Formation of an Identified Bacterial Isolate

et al. 2021

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The release of untreated tannery effluents comprising biotoxic heavy metal (HM) compounds into the ecosystem is one of our society’s most serious environmental and health issues. After discharge, HM-containing industrial effluents reach agricultural soils and thus negatively affect the soil microbial diversity. Considering these, we assessed the effect of HMs on identified soil beneficial bacteria. Here, the effects of four heavy metals (HMs), viz., chromium (Cr), cadmium (Cd), nickel (Ni), and lead (Pb), on cellular growth, physiology, cell permeability, and biofilm formation of Enterobacter cloacae MC9 (accession no.: MT672587) were evaluated. HMs in a concentration range of 25–200 μg mL–1 were used throughout the study. Among HMs, Cd in general had the maximum detrimental effect on bacterial physiology. With increasing concentrations of HMs, bacterial activities consistently decreased. For instance, 200 μgCr mL–1 concentration greatly and significantly (p ≤ 0.05) reduced the synthesis of indole-3-acetic acid (IAA) by 70% over control. Furthermore, 200 μg mL–1 Cd maximally and significantly (p ≤ 0.05) reduced the synthesis of 2,3-dihydroxybenzoic acid (2,3-DHBA), salicylic acid (SA), 1-aminocyclopropane 1-carboxylate (ACC) deaminase, and extra polymeric substances (EPSs) of E. cloacae MC9 by 80, 81, 77, and 59%, respectively, over control. While assessing the toxic effect of HMs on the P-solubilizing activity of E. cloacae, the toxicity pattern followed the order Cr (mean value = 94.6 μg mL–1) > Cd (mean value = 127.2 μg mL–1) > Pb (mean value = 132.4 μg mL–1) > Ni (mean value = 140.4 μg mL–1). Furthermore, the colony-forming unit (CFU) count (Log10) of strain MC9 was completely inhibited at 150, 175, and 200 μg mL–1 concentrations of Cr and Cd. The confocal laser scanning microscopic (CLSM) analysis of HM-treated bacterial cells showed an increased number of red-colored dead cells as the concentration of HMs increased from 25 to 200 μg mL–1. Likewise, the biofilm formation ability of strain MC9 was maximally (p ≤ 0.05) inhibited at higher concentrations of Cd. In summary, the present investigation undoubtedly suggests that E. cloacae strain MC9 recovered from the HM-contaminated rhizosphere endowed with multiple activities could play an important role in agricultural practices to augment crop productivity in soils contaminated with HMs. Also, there is an urgent need to control the direct discharge of industrial waste into running water to minimize heavy metal pollution. Furthermore, before the application of HMs in agricultural fields, their appropriate field dosages must be carefully monitored.

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“…Today, it is known that increased antimicrobial resistance induced by the overuse of antibiotics represents a global public health challenge (Llor and Bjerrum, 2014). Issues associated with biofilm occurrence, as the predominant mode of microbial growth, and also one of the key survival strategies in which microbes can resist a number of chemical and physical stresses (Altaf et al, 2021), led to the growing demand for new antimicrobial agents, especially those of natural origin (Vaou et al, 2021). The main goal of this investigation was to evaluate the antimicrobial potential of ethanolic and methanolic extract made from sweet cherry stems, as well as to assess the impact of these extracts on the biofilm-forming capacity of different bacterial and fungal species.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the antimicrobial and antibiofilm activity of sweet cherry (Prunus avium L.) stems extracts

Mahmutović-Dizdarević

Salihović

Sopić

et al. 2022

GenApp

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Sweet cherry (Prunus avium L.) stems in the form of infusions and decoctions are traditionally consumed for diuretic and anti-inflammatory purposes. This study aimed to evaluate antimicrobial and antibiofilm activity of ethanolic and methanolic extract made from sweet cherry stems. Extracts are obtained by the Soxhlet extraction and maceration procedures. For the determination of the minimum inhibitory concentration, the broth microdilution method is employed, and the assessment of the microbiocidal activity of the extracts is conducted. The antibiofilm activity was tested through the tissue culture plate method, which also allowed the determination of the biofilm-forming categories of investigated strains. The final step involved the calculation of the biofilm inhibition percentage. Examined extracts with the balanced activity inhibited the growth of all microorganisms, with Gram-negative bacteria being more sensitive in comparison to Gram-positive. The values of the minimum inhibitory concentration were 125 µg/ml, and 250 µg/ml, respectfully. Candida albicans was the most susceptible and the minimum inhibitory concentration of both extracts was 62.50 µg/ml. The microbiocidal activity of the extracts was not recorded. Extracts exhibited different impacts on the biofilm-forming capacity of the investigated microbes, and both inhibition and stimulation effects are noted. The percentage of the biofilm inhibition was from 14.27% to 84.78%, with the highest inhibition recorded for the multidrug-resistant Escherichia coli, treated with the ethanolic extract. Sweet cherry stems are a valuable source of natural bioactive compounds, but their usage in the treatment of microbial infections should be correctly and carefully implemented.

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Effective inhibition and eradication of pathogenic biofilms by titanium dioxide nanoparticles synthesized using Carum copticum extract

Abstract: Titanium dioxide nanoparticles inhibits and eradicates the biofilms of pathogenic bacteria.

Cited by 27 publications

References 58 publications

Benefits of Usage of Immobilized Silver Nanoparticles as Pseudomonas aeruginosa Antibiofilm Factors

Benefits of Usage of Immobilized Silver Nanoparticles as Pseudomonas aeruginosa Antibiofilm Factors

Heavy Metals Induced Modulations in Growth, Physiology, Cellular Viability, and Biofilm Formation of an Identified Bacterial Isolate

Evaluation of the antimicrobial and antibiofilm activity of sweet cherry (Prunus avium L.) stems extracts

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