Benefits of Usage of Immobilized Silver Nanoparticles as Pseudomonas aeruginosa Antibiofilm Factors

Abstract

The aim of this study was to assess the beneficial inhibitory effect of silver nanoparticles immobilized on SiO2 or TiO2 on biofilm formation by Pseudomonas aeruginosa—one of the most dangerous pathogens isolated from urine and bronchoalveolar lavage fluid of patients hospitalized in intensive care units. Pure and silver doped nanoparticles of SiO2 and TiO2 were prepared using a novel modified sol-gel method. Ten clinical strains of P. aeruginosa and the reference PAO1 strain were used. The minimal inhibitory concentration (MIC) was determined by the broth microdilution method. The minimal biofilm inhibitory concentration (MBIC) and biofilm formation were assessed by colorimetric assay. Bacterial enumeration was used to assess the viability of bacteria in the biofilm. Silver nanoparticles immobilized on the SiO2 and TiO2 indicated high antibacterial efficacy against P. aeruginosa planktonic and biofilm cultures. TiO2/Ag0 showed a better bactericidal effect than SiO2/Ag0. Our results indicate that the inorganic compounds (SiO2, TiO2) after nanotechnological modification may be successfully used as antibacterial agents against multidrug-resistant P. aeruginosa strains.

Hybrid Nanosystems Based on Nicotinate-Functionalized Mesoporous Silica and Silver Chloride Nanoparticles Loaded with Phenytoin for Preventing Pseudomonas aeruginosa Biofilm Development

“…Importantly, our phenytoin-loaded materials were able to significantly block the ability of P. aeruginosa to induce biofilm formation, at concentrations as low as 125–500 µg/mL (0.40–1.76 µg/mL in reference to Ag%). These values are similar to those reported with similar silver systems [ 41 ]. Nevertheless, the amount of silver in this work was 35 times lower, thus showing a higher efficiency of the final system, although these materials were not able to eradicate the biofilm, at least at the tested concentrations.…”

“…Thus, using a concentration of 125 µg/mL, biofilm development was inhibited by 80–96%, which was proven to arise from the AgCl content. These results remarkably surpass those found with MSN-based materials [ 42 , 43 ], including those systems functionalized with silver nanoparticles and nanocomposites [ 41 , 44 ].…”

The Release of Non-Extractable Ferulic Acid from Cereal By-Products by Enzyme-Assisted Hydrolysis for Possible Utilization in Green Synthesis of Silver Nanoparticles

“…The resistance of P . aeruginosa is due to the strong ability of this MDR species to produce a biofilm that protects them from harm as reported by Korzekwa et al [ 7 ]. The observed data are consistent with those of El Din et al [ 69 ], highlighting exceptionally low susceptibility of clinical MDR isolate P. aeruginosa to the AgNPs with observed MIC and MBC values as high as 8.0 mg mL −1 .…”

“…in the era of antimicrobial resistance (AMR) [ 4 , 5 ]. Among the most evolved nanotechnological applications, metallic nanoparticles (NPs) demonstrate the most unique features and versatile applications in various fields and environmental remediations [ 6 , 7 , 8 ]. In recent years, AgNPs in particular have attracted tremendous attention among the scientific community due to their superb ability to inhibit the growth of MDR bacteria and cause severe damage to cancer cells, leading to necrosis or ultimate apoptosis [ 9 , 10 ].…”

Biological properties of Cu-bearing and Ag-bearing titanium-based alloys and their surface modifications: A review of antibacterial aspect

“…It is generally believed that the main antibacterial mechanism is the interaction between bacteria and ions released from the NPs (Panácek et al, 2006;Mcquillan et al, 2012;Dorobantu et al, 2015). Under humid conditions, the ions released from NPs can induce excess ROS generation (Applerot et al, 2009;Wang et al, 2017;Korzekwa et al, 2021), which can kill bacteria by destroying intracellular biomolecules (Choi and Zhiqiang, 2008). The possible antibacterial mechanisms of NPs are shown in Figure 4.…”