A new efficient synthesis of 6,12-dihydro-5H-quinazolino[3,2-a]quinazolin-5-ones by sequential Ugi/Staudinger/aza-Wittig/addition/nucleophilic acyl substitution has been developed. -Amino amides 4, obtained from Ugi-3CR of 2-azidobenzaldehydes, methyl 2-aminobenzoates and isocyanides in the presence of catalytic amount of phenylphosphinic acid, reacted with triphenyl phosphine and isocyanates to give 6,12-dihydro-5H-quinazolino[3,2-a]quinazolin-5-ones 8 in good yields. This method provide a domino and effictive strategy for preparation of various substituted quinazolino[3,2-a]quinazolin-5-ones under mild reaction condition.