Effective, safe nonviral gene transfer to preserve the chondrogenic differentiation potential of human mesenchymal stem cells

Abstract

The data indicate that safe, efficient transgene expression can be achieved in hMSCs over time using the nonviral GeneJammer® compound, showing promise for future therapeutic settings aiming to treat human articular cartilage disorders.

Exogenous expression ofIL-1RaandTGF-β1promotes in vivo repair in experimental rabbit osteoarthritis

“…The increased therapeutic efficacy, in our study, may be due to the high level of gene expression in cartilage cells achieved by liposome-mediated delivery of recombinant plasmid DNAs (10). Our study verified that the target genes were delivered to the joints, transferred to the synovial cells, which expressed the active product, and the gene product diffused from the synovium to the articular cartilage, functionally influencing the metabolism of cartilage cells (46). Importantly, our in-vivo experiments confirmed that the efficiency of double transfection in promoting functional recovery was better than either of the single transfections.…”

The potential of 3-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with bone marrow mesenchymal stem cells for in vitro cartilage tissue engineering

“…However, again, due to limitation in resources, this present study is dedicated to evaluate immunohistochemistry for collagen type I and II only. Furthermore, many previous papers are showing on immunohistochemistry for collagen type II only (Cao et al, 2011;Elsler et al, 2012;Feng et al, 2011;Song et al, 2013;Wu et al, 2014;Yang et al, 2012).…”

Delivery of the Sox9 gene promotes chondrogenic differentiation of human umbilical cord blood-derived mesenchymal stem cells in an in vitro model

“…Because the incidence of spontaneous chondrogenic differentiation of MSCs is very low, many pharmacological and genetic approaches have been developed to induce such differentiation (12). SRY-related high-mobility-group box 9 (Sox9) gene is a cartilage-specific transcription factor and plays essential roles in chondrocyte differentiation and cartilage formation (13).…”