2024
DOI: 10.3390/molecules29112461
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Effective Synthesis of High-Integrity mRNA Using In Vitro Transcription

Wei He,
Xinya Zhang,
Yangxiaoyu Zou
et al.

Abstract: : mRNA vaccines are entering a period of rapid development. However, their synthesis is still plagued by challenges related to mRNA impurities and fragments (incomplete mRNA). Most impurities of mRNA products transcribed in vitro are mRNA fragments. Only full-length mRNA transcripts containing both a 5′-cap and a 3′-poly(A) structure are viable for in vivo expression. Therefore, RNA fragments are the primary product-related impurities that significantly hinder mRNA efficacy and must be effectively controlled; … Show more

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