Effectiveness of Addition Papain Enzyme in Artificial Diet on the Metamorphosis Rate and Glycogen Content of Mangrove Crab Larvae (Scylla olivacea)

Abstract

In the rearing of fish larvae, artificial feed can be given when digestive enzymes have been produced. To improve the ability of fish larvae to utilize artificial feeds can be done with the addition of exogenous enzyme into the artificial feeds. The aim of this study was to determine the dose of papain enzyme and the correct predigest artificial feeding stadium on metamorphosis rate and glycogen content of mangrove larvae (Scylla olivacea). The experimental design used was a factorial pattern with a complete randomized baseline design, the first factor being the dose of papain enzyme (0%, 1.5%, 3.0% and 4.5%), while the second factor was the predigest artificial feeding stage (zoea 2 and 3). The results showed that the difference of dose of papain enzyme and predigest artificial feeding stages had significant effect (p <0.05) on the metamorphosis rate of 7, 9, 10, 12, 13, 16 and 17 day larvae, but the interaction between them was not significant (p>0,05). The rate of metamorphosis at doses of enzyme papain was 0% and 1.5% longer and significantly different (p <0.05) compared to 3% and 4.5% doses. The rate of metamorphosis at doses of 3.0% and 4.5% was not significantly different (p> 0.05). The content of glycogen larvae in dose of enzyme papain 4,5% not different than dose 1,5% and 3,0% but higher and significantly different than dose 0%. Glycogen content at dose 0%, 1,5% and 3,0%. was not significantly different (p> 0.05). Based on the results of these studies to hydrolyze feed proteins can be used papain enzyme with a dose of 4.5%.Keywords: papain enzyme, metamorphosis rate, mangrove crab larvae (Scylla olivacea), glycogen content

The effectiveness of Papain Enzyme Supplementation in Artificial Feed on Growth and Survival Rate of Mangrove Crab Larvae (Scylla Olivacea)

“…The lowest of total amino acid content was also at a dose of enzyme papain 0.0%, i.e. only 29.92%, whereas, at a dose of papain enzyme 1.5%, 3.0% and 4.5% were 36.47%, 37.26%, and 36.04% respectively [11]. The results of Kurniawan et al [17] also showed the same pattern, the amino acid content of the squid ink sample without the addition of papain enzyme was also lower than the amino acid content of the hydrolysed sample with the addition of 2%.…”

“…The result of analysis soluble protein content and protein hydrolysis levels showed that in the artificial diet which hydrolysis with enzyme papain 0% and 1,5% significant different but was lowest and insignificantly different compared to a dose of enzyme papain 3.0% and 4,5%. The content of soluble protein at a dose of enzyme papain of 3.0% and 4.5% was not different [11]. The low content of dissolved protein at the dose of papain enzyme was thought to be due to the limited concentration of enzymes that cannot hydrolyse the protein well…”

“…An easily digestible feed will produce energy supplies, energy will be used for maintenance and activity, and excess energy will be used for growth. Four percent of supplementation of papain to the artificial diet at the age of 12 and 15 days Silver Pompano larvae resulted in better protease enzyme activity [11]. Supplements of bovine trypsin resulted in better growth for P. Japonicus.…”

“…Larva is able to utilize artificial feed at the time the enzyme has been produced. The results of Haryati et al [11] study showed that the greatest relative changes in the activity of trypsin, lipase and α-amylase enzyme were the earliest in mud crab (Scylla olivaacea) larvae from Zoea II stadium to Zoea III. Based on these studies, artificial feed is thought to be given starting Zoea III.…”

“…Furthermore, the results of the Haryati et al [11] study showed that the survival rates of mud crab larvae (Scylla olivacea) from Zoea I stadium to megalopa fed artificial feed started Zoea II stadia was lowest compared to those still fed with natural and artificial feed which begin at Zoea III and IV. Efforts to accelerate the use of artificial feed can be done by predigest feed using exogenous enzymes.…”