Effectiveness of an Attenuated Zucchini yellow mosaic virus Isolate for Cross-Protecting Cucumber

Abstract

To cross-protect cucumber plants from Zucchini yellow mosaic virus (ZYMV), we used cold treatment to obtain an attenuated isolate of ZYMV, designated ZYMV-2002. ZYMV-2002 was obtained from a virulent ZYMV isolate after repeated low temperature treatment at 12.5 to 15°C followed by five cycles of single-plant transfer. The isolate produced very mild or no symptoms on cucurbit plants. In addition, inoculated cucumber plants had very similar fruit productivity to healthy control plants under field conditions. During field experiments in 2002 and 2003, when other viruses were also present, protected plants significantly suppressed infection with ZYMV, progression of disease severity, and reduction of fruit yield and quality. These results demonstrate that ZYMV-2002 is a potentially useful attenuated ZYMV isolate for reducing the impact of ZYMV.

Genetic analysis and gene mapping of papaya ring spot virus resistance in cucumber

“…Cucurbit plantings are particularly vulnerable from the end of summer through early autumn, when increased aphid vector populations promote virus epidemics that eventually induce severe losses in fruit yield (Kosaka et al 2006). No chemical treatment has yet been found to effectively control the virus in infected plants, and thus genetic resistance against the virus is the optimal method for PRSV-W control.…”

Highly activated RNA silencing via strong induction of dicer by one virus can interfere with the replication of an unrelated virus

“…In antagonistic interactions, a preinfecting virus, usually a very mild strain, incites RNA silencing and interferes with the replication of a closely related severe strain of the same virus (14). This phenomenon is also referred to as cross-protection, and some attenuating plant viruses have been commercialized as biological control agents (15). Most, if not all, cross-protection phenomena in plants are shown to be due to RNA silencing (14,16).…”

Synergy between Cucumber Mosaic Virus and Zucchini Yellow Mosaic Virus on <italic>Cucurbitaceae</italic> Hosts Tested by Real-time Reverse Transcription-Polymerase Chain Reaction

“…An attenuated strain of ZYMV-WK was used widely as a bio-control agent in many areas in Europe and Japan [27−29], but its interaction with CMV as an epidemic virus in the same ecological position has not been mentioned. Kosaka et al also used many attenuated strains of ZYMV to control viral infection successfully and enhance resistant ability against the virus on cucumber and other plants [30,31]. But, according to our present work, the accumulation of CMV increased rapidly in the co-infection.…”

“…In parallel to this test, three cultivars of bottle gourd (L. siceraria cv. Yonghu 2,15,31) were grown in the field and used for the disease index test, so that there were 30 plants for each treatment. Isolates, CMV-Fny and ZYMV-SD, were inoculated and maintained on seedlings of tobacco and bottle gourd respectively, then biologically assayed for their infectious virility.…”