Effectiveness of different disinfection techniques of the root canal in the elimination of a multi-species biofilm

Abstract

BackgroundThe purpose of the study was to evaluate the effectiveness of different root canal disinfection techniques in the elimination of a multi-species biofilm from inside the root canal.Material and MethodsFifty mandibular first premolars were used in the present study, standardized to 11mm of root length, and instrumented with a reciprocation system Reciproc, (VDW GmbH, Munich, Germany) to a #50. Longitudinally sectioned halves of the roots were obtained and washed with NaOCl 4%, EDTA 17% and 5% sodium thiosulfate, and sterilized by autoclaving for 15 minutes at 121°C. A multi-species biofilm broth was developed with three strains of bacteria under laboratory conditions: Enterococcus faecalis ATTC 29212, Eikenella corrodens ATTC 23834, Streptococcus anginosus ATTC 33397. Roots were autoclaved and transferred to the broth for 4 days and then were subjected to either disinfection with sodium hypochlorite 4% and XP-endo Finisher (FKG Dentaire, La Chaux-de-Fonds, Switzerland) or chlorhexidine 2% with and without activation with XP-endo Finisher (FKG Dentaire, La Chaux-de-Fonds, Switzerland).ResultsThe evaluations of the biofilm elimination showed results that indicate that the 4% sodium hypochlorite group with positive pressure irrigation presented significant differences with the group that had irrigation with sodium hypochlorite activated with XP-endo Finisher and the chlorhexidine groups to 2% (P<0.05).ConclusionsChlorhexidine 2% activated with the XP-endo Finisher does not exert elimination or improved cleaning effect on the multi-species biofilm. Activation of sodium hypochlorite 4% improved the elimination of the multi-species biofilm. Key words:Biofilm, multispecies, chlorhexidine, sodium hypochlorite.

Evaluation of Photodynamic Therapy Using a Diode Laser 635 nm as an Adjunct to Conventional Chemo-Mechanical Endodontic Procedures against Enterococcus faecalis Biofilm: Ex-Vivo Study

“…Irrigation is a complementary procedure of instrumentation in endodontic treatment. It facilitates and contributes in the elimination of pulp tissue, the smear layer, and the remaining biofilm [34]. The most commonly used and studied irrigants are NaOCl and chlorhexidine (CHX) [35].…”

“…CHX has been proposed as an alternative of NaOCl for open apex cases and patients with NaOCl allergy [37]. The major disadvantage of CHX is its limited ability to dissolve organic tissues [34].…”

Silver nanoparticles in endodontics: recent developments and applications

“…Biofilms are highly organized, surface-adherent structures of microcolonies [ 16 ] ( Figure 2 ). The main component of biofilms is an exopolymeric matrix consisting of polysaccharides, proteins, enzymes, and bacterial metabolites [ 17 18 ]. Exopolysaccharides are synthesized both intracellularly and extracellularly, and have skeletal functions [ 19 ] ( Figure 3 ).…”

The effect of various in-vitro and ex-vivo parameters on irrigant flow and apical pressure using manual syringe needle irrigation: Systematic review

“…Negligence to one or the other aspect might lead to the failure of the treatment. The most studied and still neglected part of the endodontic treatment is the disinfection of the root canal space ( Eckhardt et al, 2010 , Teves et al, 2019 ). A three-dimensionally obturated root canal space is reciprocating three-dimensional disinfection and cleaning of the root canal system.…”