Effectiveness of ear skin swabs for monitoring methicillin-resistant &lt;i&gt;Staphylococcus aureus&lt;/i&gt; ST398 in pigs at abattoirs

Sasaki, Yohei; Sakurada, Haruko; Yamanaka, Makiko; Nara, Kazuhisa; Tanaka, Shigeko; Uema, Masashi; Ishii, Yoshikazu; Tsutsumi, Yutaka; Asai, Tetsuo

doi:10.1292/jvms.20-0592

Cited by 5 publications

(10 citation statements)

References 19 publications

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“…A greater level of agreement (0.7; good agreement) had been indicated between these sample types from a comparative farm study [ 22 ]. However, a similar level of agreement (0.4; moderate) had been shown by a previous abattoir study which assessed individual pigs [ 23 ], and this may indicate the importance of collecting both sample types from pigs to maximise the ability to detect MRSA at slaughter.…”

Section: Discussionsupporting

confidence: 71%

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Livestock-associated methicillin-resistant Staphylococcus aureus in slaughtered pigs in England

et al. 2021

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This study was performed to investigate the occurrence of livestock-associated methicillinresistant Staphylococcus aureus (LA-MRSA) in batches of pigs at slaughter and at different stages along the slaughter line. Nasal and ear skin swabs were collected from 105 batches of ten pigs at six abattoirs. Cultures (pooled or individual) were performed for MRSA using selective media; presumptive MRSA were confirmed by mecA and nuc gene detection and a selection were spa-typed. MRSA was detected in 46 batches. All spatypes detected were those associated with LA-MRSA clonal complex 398. The proportion of positive batches varied between abattoirs (0-100%). Two abattoirs were subsequently further investigated, with samples taken at post-stunning, chiller, and either at lairage or post-singe. Results suggested cross-contamination occurred between the lairage and point of post-stunning, but the slaughter processes appeared effective in reducing contamination before carcasses entered the chiller. One abattoir provided only negative samples in the initial study and in the subsequent study along the slaughter line (26 batches in total), suggesting differences possibly in the MRSA status of pigs on arrival from supply farms or in its abattoir practices affecting the MRSA status of pigs at the sampling points. This study highlights that in the investigated abattoirs, MRSA was detected in 43.8% of batches of pigs at slaughter using sensitive selective culture methods..

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Section: Discussionsupporting

confidence: 71%

“…The relative sensitivity of different sampling sites in pigs and on farms has been investigated [21][22][23]. These investigations found that sampling both nasal swabs and ear skin swabs had a higher relative sensitivity (98.2%) than sampling either site alone, or sampling perineum, or other combinations of these three sites.…”

Section: Introductionmentioning

confidence: 99%

Livestock-associated methicillin-resistant Staphylococcus aureus in slaughtered pigs in England

et al. 2021

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“…Ears contaminated with ST398 MRSA originated from pigs in the Tohoku and Kanto regions. In contrast to the characteristics of ST398 MRSA reported in the two regions [ 20 , 21 , 22 ], the characteristics of the isolates in the present study were different. Fifty-eight ST398 MRSA isolates were obtained in the Kanto region between 2012 and 2014, and the most frequent spa type was t16450 (33 isolates), followed by t034 (14 isolates), and t3934 (11 isolates) [ 20 ].…”

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confidence: 99%

“…The core-genome phylogenetic analysis showed relatively small numbers of single nucleotide polymorphisms (SNPs) (range, [13][14][15][16][17][18][19][20][21][22][23][24][25][26] in the seven ST398 MRSA isolates (a-1, a-2, b-1, c-1, d-1, e-1, and f-1) carrying SCCmec type IVd (Fig. 1).…”

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confidence: 99%

First isolation of ST398 methicillin-resistant <i>Staphylococcus aureus</i> carrying staphylococcal cassette chromosome <i>mec</i> type IVd from pig ears in Japan

Sasaki

Aoki

Ishii

et al. 2022

The Journal of Veterinary Medical Science

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The emergence and increasing prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) are a global concern. To investigate the prevalence and characteristics of sequence type 398 (ST398) MRSA in pig ears, 102 pig's ears were collected from 102 animals shipped from 51 farms at an abattoir. Eight ST398 MRSA isolates were isolated from the ears of eight pigs shipped from seven farms. Of the eight ST398 isolates, seven had the staphylococcal cassette chromosome mec (SCCmec) type IVd and these were obtained from seven pigs shipped from six farms. Single nucleotide polymorphisms ranging from 13 to 26 were observed in the core-genome regions in the seven SCCmec type IVd isolates. We believe that this is the first report on the isolation of ST398 MRSA SCCmec type IVd in Japan.

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“…The geographic range of LA-MRSA ST398 is expanding. It has been reported in pigs and humans in recent years in Korean and Japan ( Lee et al, 2021 ; Nakaminami et al, 2021 ; Sasaki et al, 2021 ). LA-MRSA ST398 isolates were recovered from milk samples from two farms in China in one study.…”

Section: Concentrated Animal Feeding Operations and Transmission Of M...mentioning

confidence: 94%

Association of Common Zoonotic Pathogens With Concentrated Animal Feeding Operations

et al. 2022

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Animal farming has intensified significantly in recent decades, with the emergence of concentrated animal feeding operations (CAFOs) in industrialized nations. The congregation of susceptible animals in CAFOs can lead to heavy environmental contamination with pathogens, promoting the emergence of hyper-transmissible, and virulent pathogens. As a result, CAFOs have been associated with emergence of highly pathogenic avian influenza viruses, hepatitis E virus, Escherichia coli O157:H7, Streptococcus suis, livestock-associated methicillin-resistant Staphylococcus aureus, and Cryptosporidium parvum in farm animals. This has led to increased transmission of zoonotic pathogens in humans and changes in disease patterns in general communities. They are exemplified by the common occurrence of outbreaks of illnesses through direct and indirect contact with farm animals, and wide occurrence of similar serotypes or subtypes in both humans and farm animals in industrialized nations. Therefore, control measures should be developed to slow down the dispersal of zoonotic pathogens associated with CAFOs and prevent the emergence of new pathogens of epidemic and pandemic potential.

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Effectiveness of ear skin swabs for monitoring methicillin-resistant <i>Staphylococcus aureus</i> ST398 in pigs at abattoirs

Cited by 5 publications

References 19 publications

Livestock-associated methicillin-resistant Staphylococcus aureus in slaughtered pigs in England

Livestock-associated methicillin-resistant Staphylococcus aureus in slaughtered pigs in England

First isolation of ST398 methicillin-resistant <i>Staphylococcus aureus</i> carrying staphylococcal cassette chromosome <i>mec</i> type IVd from pig ears in Japan

Association of Common Zoonotic Pathogens With Concentrated Animal Feeding Operations

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