Effectiveness of FMT in recurrent Clostridium difficile infection

Grzesiowski, P.; Hermann, Anne Pernille; Dubaniewicz, Anna; Kasprzyk, Joanna; Pawlik, Dorota; Zak-Pulawska, Z

doi:10.1186/2047-2994-4-s1-p27

Cited by 2 publications

(3 citation statements)

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“…Similarly, in Poland Grzesiowski et al [73] obtained the curative rate of 55/62 (88.7%) in recurrent CDI (rCDI); 76.5% of cures occurred after the first course of treatment, 14.5% after the second, and the remaining 9% after the third infusion through the nasogastric tube. The results by Grzesiowski are particularly important because they indicate that in most cases CDI treatment should be planned with implementation of FMT cycles (not just a single transplant), bringing the expected curative rate to nearly 100%.…”

Section: Discussionmentioning

confidence: 90%

Guidelines for Clostridium difficile infection in adults

Kukla

Adrych

Dobrowolska

et al. 2020

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Clostridium difficile infection (CDI) has become a serious medical and epidemiological problem, especially in well developed countries. There has been evident increase in incidence and severity of CDI. Prevention, proper diagnosis and effective treatment are necessary to reduce the risk for the patients, deplete the spreading of infection and diminish the probability of recurrent infection. Antibiotics are the fundamental treatment of CDI. In patients who had recurrent CDI fecal microbiota transplantation seems to be promising and efficient strategy. These guidelines systematize existing data and include recent changes implemented in the management of CDI. Guidelines for Clostridium difficile infection in adults

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Section: Discussionmentioning

confidence: 90%

Guidelines for Clostridium difficile infection in adults

Kukla

Adrych

Dobrowolska

et al. 2020

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“…Nevertheless, FMT with frozen material still appears to deliver satisfactory results, as the whole stool freezing approach is routinely used in one of the polish stool banks with an overall CDI cure rate approx. 90% (Grzesiowski et al, 2015). We also consider it very important to note that the lack of characterization of cells from the Unknown group, and especially from the group of "double negative" cells, does not allow us to clearly state whether the viability of bacterial cells has actually decreased, whether it has not changed or even increased during freezing.…”

Section: Discussionmentioning

confidence: 99%

“…It is, however, postulated that glycerol can skew intestine microbiota composition and other natural methods should be applied (De Weirdt et al, 2010). Freezing the feces alone, without adding cryoprotectants, is the most common practice when collecting patient samples, but to the authors' knowledge, it is also practiced in stool storage for the production of fecal microbiota preparations for transfer into the gastrointestinal tract of the recipient now, not only historically (Gustafsson et al, 1999) but also with very good results not differing from others (Grzesiowski et al, 2015). Different cryoprotectants have been described and tested, for example, freeze-drying FMT capsules (Staley et al, 2017;Burz et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Fresh Versus Frozen Stool for Fecal Microbiota Transplantation—Assessment by Multimethod Approach Combining Culturing, Flow Cytometry, and Next-Generation Sequencing

Biliński

Dziurzyński

Grzesiowski³

et al. 2022

Front. Microbiol.

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The objective of this work was to compare the quality of FMT preparations made from fresh feces with those made from feces frozen at –30°C without any pre-processing or cryopreservation additives. The research hypothesis was that such preservation protocol (frozen whole stool, then thawed and processed) is equipotent to classical fresh FMT preparation. For that, three complementary methods were applied, including: (i) culturing in aerobic and anaerobic conditions, (ii) measuring viability by flow cytometry, and (iii) next-generation sequencing. Flow cytometry with cell staining showed that the applied freezing protocol causes significant changes in all of the observed bacterial fractions. Alive cell counts dropped four times, from around 70% to 15%, while the other two fractions, dead and unknown cell counts quadrupled and doubled, with the unknown fraction becoming the dominant one, with an average contribution of 57.47% per sample. It will be very interesting to uncover what this unknown fraction is (e.g., bacterial spores), as this may change our conclusions (if these are spores, the viability could be even higher after freezing). Freezing had a huge impact on the structure of cultivable bacterial communities. The biggest drop after freezing in the number of cultivable species was observed for Actinobacteria and Bacilli. In most cases, selected biodiversity indices were slightly lower for frozen samples. PCoA visualization built using weighted UniFrac index showed no donor-wise clusters, but a clear split between fresh and frozen samples. This split can be in part attributed to the changes in the relative abundance of Bacteroidales and Clostridiales orders. Our results clearly show that whole stool freezing without any cryoprotectants has a great impact on the cultivability and biodiversity of the bacterial community, and possibly also on the viability of bacterial cells.

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Effectiveness of FMT in recurrent Clostridium difficile infection

Cited by 2 publications

References 0 publications

Guidelines for Clostridium difficile infection in adults

Guidelines for Clostridium difficile infection in adults

Fresh Versus Frozen Stool for Fecal Microbiota Transplantation—Assessment by Multimethod Approach Combining Culturing, Flow Cytometry, and Next-Generation Sequencing

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