Effectiveness of halo-tolerant, auxin producing Pseudomonas and Rhizobium strains to improve osmotic stress tolerance in mung bean (Vigna radiata L.)

Abstract

Halo-tolerant, auxin producing bacteria could be used to induce salt tolerance in plants. A number of Rhizobium and auxin producing rhizobacterial strains were assessed for their ability to tolerate salt stress by conducting osmoadaptation assay. The selected strains were further screened for their ability to induce osmotic stress tolerance in mung bean seedlings under salt-stressed axenic conditions in growth pouch/jar trials. Three most effective strains of Rhizobium and Pseudomonas containing ACC-deaminase were evaluated in combination, for their ability to induce osmotic stress tolerance in mung bean at original, 4, and 6 dS m−1 under axenic conditions. Results showed that sole inoculation of Rhizobium and Pseudomonas strains improved the total dry matter up to 1.4, and 1.9 fold, respectively, while the increase in salt tolerance index was improved up to 1.3 and 2.0 fold by the Rhizobium and Pseudomonas strains, respectively. However, up to 2.2 fold increase in total dry matter and salt tolerance index was observed due to combined inoculation of Rhizobium and Pseudomonas strains. So, combined application of Rhizobium and Pseudomonas strains could be explored as an effective strategy to induce osmotic stress tolerance in mung bean.

Field application of ACC-deaminase biotechnology for improving chickpea productivity in Bahawalpur

“…It is generally grown on marginal lands (sandy soils) under irrigation conditions and in rainfed areas where water scarcity and increasingly variable and extreme weather conditions are the main problems. The ACC deaminase containing rhizobacterial based biofertilizers have been well documented to help crop plants to withstand extreme environmental conditions (Arshad et al, 2008;Ahmad et al, 2013b;Nadeem et al, 2014). The present study thus explores the effectiveness of multi-strain ACC deaminase Means sharing same letters are statistically show insignificant results at par at 5 % level of probability (n = 3) containing biofertilizer to improve the productivity of chickpea under field conditions in Bahawalpur region where water availability is the major problem.…”

“…These rhizobia facilitate the growth of their host plant by fixing atmospheric nitrogen, antibiotics production, siderophores production, and solubilization of minerals (Patten and Glick, 2002). The combined use of these rhizobia and PGPR can be an efficient an environmental friendly approach to improve the growth and productivity of crop plants under unfavourable environmental conditions (Selvakumar et al, 2012;Ahmad et al, 2013b;Nadeem et al, 2014). Thus, the use of multistrain biofertilizers containing efficient strains of ACC deaminase producing rhizobacteria and Rhizobia can be a resourceful biotechnology for improving growth and productivity of chickpea under limited availability of water.…”

Volatile-Mediated Effects Predominate in Paraburkholderia phytofirmans Growth Promotion and Salt Stress Tolerance of Arabidopsis thaliana

“…Over the last few years, several studies have reported the ability of isolated microorganisms to induce plant tolerance to salinity once they have been inoculated to seeds or young plantlets (reviewed in Yang et al, 2009; Dodd and Pérez-Alfocea, 2012; Shrivastava and Kumar, 2015), including a variety of hosts, like wheat (Nadeem et al, 2013; Singh et al, 2015), maize (Hamdia et al, 2004; Nadeem et al, 2009), cotton (Liu et al, 2013; Egamberdieva et al, 2015), tomato (Mayak et al, 2004; Ali et al, 2014), lettuce (Barassi et al, 2006; Kohler et al, 2009), sunflower (Shilev et al, 2010; Tewari and Arora, 2014) and Arabidopsis (Zhang et al, 2008; Kim et al, 2014; Sukweenadhi et al, 2015). Among the PGPR that have been demonstrated to play a role in salt stress tolerance induction, a wide diversity of bacteria is included, encompassing several members of the γ-proteobacteria class, specially within the genus Pseudomonas (Ahmad et al, 2013; Nadeem et al, 2013; Chang et al, 2014; Han et al, 2015), α-proteobacteria belonging to the Azospirillum genus (del Amor and Cuadra-Crespo, 2011; Nia et al, 2012; Sahoo et al, 2014), and β-proteobacteria like Achromobacter (Mayak et al, 2004) or Paraburkholderia (Talbi et al, 2013; Pinedo et al, 2015). Several examples have also been described for the phylum Firmicutes, with special emphasis on the genus Bacillus (Zhang et al, 2008; Kohler et al, 2009; Karlidag et al, 2013; Ramadoss et al, 2013; Han et al, 2015), and also some examples have been described within the Actinobacteria phylum (Sadeghi et al, 2012; Palaniyandi et al, 2014; Gond et al, 2015).…”

Beans with Benefits—The Role of Mungbean (<i>Vigna radiate</i>) in a Changing Environment

“…It has been well-established that Rhizobium strains isolated from salt affected fields are more tolerant to salinity and can improve growth of mungbean under salt-stressed conditions [111] [112] [113]. Inoculation with rhizobia enhances root proliferation and the number of primary roots under salinity stress, leading to improved growth and yield, through a number of mechanisms such as N 2 fixation, production of plant growth regulators, and disease suppression [114].…”