Effectiveness of lipopolysaccharide as an intrauterine immunomodulator in curing bacterial endometritis in repeat breeding cross-bred cows

Singh, Jagir; Sidhu, S. S.; Dhaliwal, G. S.; Pangaonkar, G. R.; Nanda, Annu; Grewal, A. S.

doi:10.1016/s0378-4320(00)00144-5

Cited by 33 publications

(27 citation statements)

References 7 publications

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“…Several research groups have made some attempts to sensitize the detection of LPS. Preventive administration of LPS has been done before as described by Singh et al (2000) who used LPS as an intrauterine immunomodulator to cure bacterial endometritis in cattle [138]. Intrauterine administration of LPS increased the local amount of leukocytes, of which 80% were PMN.…”

Section: Tlr-4 Agonistsmentioning

confidence: 99%

The toll-like receptor-4 (TLR-4) pathway and its possible role in the pathogenesis ofEscherichia colimastitis in dairy cattle

et al. 2007

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-Mastitis is one of the most costly production diseases in the dairy industry that is caused by a wide array of microorganisms. In this review, we focus on the Gram-negative Escherichia coli infections that often occur at periods when the innate immune defence mechanisms are impaired (i.e., parturition through the first 60 days of lactation). There is substantial evidence demonstrating that at these periods, the expected influx of polymorphonuclear neutrophil leukocytes (PMN) into the mammary gland is delayed during inflammation after intramammary infection with E. coli. Here, we provide some hypotheses on the potential mechanisms of action on how the disease may develop under circumstances of immunosuppression, and describe the potential involvement of the toll-like receptor-4 signal transduction pathway in the pathogenesis of E. coli mastitis. In addition, some ideas are proposed to help prevent E. coli mastitis and potentially other diseases caused by Gram-negative infections in general.

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Section: Tlr-4 Agonistsmentioning

confidence: 99%

The toll-like receptor-4 (TLR-4) pathway and its possible role in the pathogenesis ofEscherichia colimastitis in dairy cattle

et al. 2007

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“…Recently, the intrauterine administration of LPS in cattle was shown to facilitate clearance of chronic intrauterine infections associated with infertility (34). However, despite these potentially beneficial effects, the pharmacologic use of purified LPS (or lipid A) is precluded by its extreme toxicity; LPS is highly pyrogenic and promotes systemic inflammatory response syndrome (12).…”

mentioning

confidence: 99%

Synthetic Toll-Like Receptor 4 Agonists Stimulate Innate Resistance to Infectious Challenge

et al. 2005

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A compound family of synthetic lipid A mimetics (termed the aminoalkyl glucosaminide phosphates [AGPs]) was evaluated in murine infectious disease models of protection against challenge with Listeria monocytogenes and influenza virus. For the Listeria model, intravenous administration of AGPs was followed by intravenous bacterial challenge 24 h later. Spleens were harvested 2 days postchallenge for the enumeration of CFU. For the influenza virus model, mice were challenged with virus via the intranasal/intrapulmonary route 48 h after intranasal/intrapulmonary administration of AGPs. The severity of disease was assessed daily for 3 weeks following challenge. Several types of AGPs provided strong protection against influenza virus or Listeria challenge in wild-type mice, but they were inactive in the C3H/HeJ mouse, demonstrating the dependence of the AGPs on toll-like receptor 4 (TLR4) signaling for the protective effect. Structure-activity relationship studies showed that the activation of innate immune effectors by AGPs depends primarily on the lengths of the secondary acyl chains within the three acyl-oxy-acyl residues and also on the nature of the functional group attached to the aglycon component. We conclude that the administration of synthetic TLR4 agonists provides rapid pharmacologic induction of innate resistance to infectious challenge by two different pathogen classes, that this effect is mediated via TLR4, and that structural differences between AGPs can have dramatic effects on agonist activity in vivo.The toll-like receptors (TLRs) comprise an evolutionarily conserved receptor family that is capable of detecting and responding to microbial challenge (1). The TLRs recognize a variety of pathogen-specific components, including lipopolysaccharide (LPS), CpG DNA, and microbial membrane and cell wall components (20). Toll-like receptor 4 (TLR4) is critical for the recognition of LPS (30, 31), and considerable progress has recently been made in understanding the interaction of TLR4 with critical accessory molecules implicated in LPS recognition (8,9,17,21,22, 32,40,41). In this regard, it appears that LPS binding protein (LBP) promotes the binding of LPS to CD14, which in turn facilitates the association of the lipid A component of LPS with MD-2 to form a soluble complex that serves as an activating ligand for TLR4 on the cell surface. Binding of the MD-2/LPS complex to TLR4 results in the aggregation of TLR4 into lipid rafts and the activation of several distinct intracellular signaling pathways that results in increased transcription of many genes encoding cytokines, defensins, chemokines, and alpha/beta interferons (28, 38). These effector molecules determine a wide range of biological activities, including further production of cytokines, enhancement of microbicidal activity of phagocytic cells, and migration/maturation of dendritic cells (5,23,36).Before the discovery that LPS interacts with TLR4, evidence demonstrating the importance of innate immune activation in controlling infection with gram-neg...

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“…It is possible that the breeding attempt may have stimulated clearance of the bacteria. In cows, intrauterine infusion with lipopolysaccharide from E. coli appears to enhance the uterine clearance of bacteria [40]. Christoffersen et al [41] found a positive effect of mycobacterium cell wall extract (MCWE) on the accumulation of intrauterine fluid and the clearance of uterine pathogens.…”

Section: Discussionmentioning

confidence: 99%

Equine Infectious Endometritis—Clinical and Subclinical Cases

Rasmussen

Petersen

Bojesen

et al. 2015

Journal of Equine Veterinary Science

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Effectiveness of lipopolysaccharide as an intrauterine immunomodulator in curing bacterial endometritis in repeat breeding cross-bred cows

Cited by 33 publications

References 7 publications

The toll-like receptor-4 (TLR-4) pathway and its possible role in the pathogenesis ofEscherichia colimastitis in dairy cattle

The toll-like receptor-4 (TLR-4) pathway and its possible role in the pathogenesis ofEscherichia colimastitis in dairy cattle

Synthetic Toll-Like Receptor 4 Agonists Stimulate Innate Resistance to Infectious Challenge

Equine Infectious Endometritis—Clinical and Subclinical Cases

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