Effectiveness of veterinary drug «Forvit solution of vitamins A, D, E, F in oil for injection» in complex treatment of cows with osteodystrophy

Abstract

Diseases of metabolism, in particular mineral and vitamin, are widespread among animals of various species, and lead to significant economic loses as a result of decrease in productivity.The aim of this study was to determine the effectiveness of veterinary drug «Forvit solution of vitamins A, D, E, F in oil for injection» in the complex treatment of cows with osteodystrophy.The object of the study was 8 black-and-white cows at the age of 4-6 years, with clinical signs of osteodystrophy.The diagnosis was made in a complex base; we took into account anamnestic data, clinical symptoms and laboratory tests of blood serum. Clinical studies of cows were carried out according to the generally accepted methods. After confirming the diagnosis, the animals were prescribed complex treatment. We gave 100 g of Tricalciumphosphate with grain every cow once a day during 25 days and injected intramuscularly 15 ml of «Forvit solution of vitamins A, D, E, F in oil for injection» once every 5 days, a total -5 injections.Inorganic phosphorus -by the spectrophotometric method; calcium (total and ionized) -using the AEK-01 electrolyte analyzer; alkaline phosphatase (ALP) activity according to the Bodansky method; activity of alanine (ALT) and aspartate aminotransferases (AST) -by an optimized enzymatic kinetic method (a set of reagents from Vital Diagnostics SPb); protein -by biuret reaction; cholesterol -by the Endrashik method; urea and creatinine -by the kinetic method were determined in blood serum. Mathematical processing was carried out using the Fisher-Student T-test and the standard "Statistics" package in the EXCEL program.The cause of osteodystrophy in cows was a lack of calcium (30,4 %), phosphorus (30,3%) and vitamin D (86,5 %) in the diet. Clinically, the disease was manifested by unsteadiness of the incisors, resorption of the last caudal vertebrae, and partial lysis of the last pair of ribs. Concentration of calcium and phosphorus in blood serum of cows with osteodystrophy was reduced by 11,5 % and 11,1 %, respectively, and activity of alkaline phosphatase was increased by 47,2% compared with the norm.Concentration of calcium increased by 6,9 %, and activity of alkaline phosphatase decreased by 47,4 % in blood serum of cows after using «Forvit solution of vitamins A, D, E, F in oil for injection» and Tricalciumphosphate.