Effects and mechanism of lncRNA‐27785.1 that regulates TGF‐β1 of Sika deer on antler cell proliferation

Chen, Danyang; Li, Yanjun; Jiang, Renfeng; Li, Yuting; Jiang, Feng; Hu, Wei

doi:10.1002/jcp.30258

Cited by 6 publications

(3 citation statements)

References 43 publications

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“…With the advancement of RNAomics research, more and more non-coding RNAs have been gradually discovered for their biological functions in the organism. Our previous work has preliminarily demonstrated that miRNAs and lncRNAs have important regulatory roles in the proliferation and differentiation of antler chondrocytes [ 15 , 16 , 17 ]. Circular non-coding RNAs (circRNAs) are widely present in a variety of cells and tissues and are characterized by structural stability, sequence conservation, and tissue-specific expression, and are involved in a variety of biological processes [ 18 , 19 , 20 ].…”

Section: Discussionmentioning

confidence: 99%

Whole-Transcriptome Sequencing of Antler Tissue Reveals That circRNA2829 Regulates Chondrocyte Proliferation and Differentiation via the miR-4286-R+1/FOXO4 Axis

Yao

Jiang

Chen

et al. 2023

IJMS

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The antler is the unique mammalian organ found to be able to regenerate completely and periodically after loss, and the continuous proliferation and differentiation of mesenchymal cells and chondrocytes together complete the regeneration of the antler. Circular non-coding RNAs (circRNAs) are considered to be important non-coding RNAs that regulate body development and growth. However, there are no reports on circRNAs regulating the antler regeneration process. In this study, full-transcriptome high-throughput sequencing was performed on sika deer antler interstitial and cartilage tissues, and the sequencing results were verified and analyzed. The competing endogenous RNA (ceRNA) network related to antler growth and regeneration was further constructed, and the differentially expressed circRNA2829 was screened out from the network to study its effect on chondrocyte proliferation and differentiation. The results indicated that circRNA2829 promoted cell proliferation and increased the level of intracellular ALP. The analysis of RT-qPCR and Western blot demonstrated that the mRNA and protein expression levels of genes involved in differentiation rose. These data revealed that circRNAs play a crucial regulatory role in deer antler regeneration and development. CircRNA2829 might regulate the antler regeneration process through miR-4286-R+1/FOXO4.

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Section: Discussionmentioning

confidence: 99%

Whole-Transcriptome Sequencing of Antler Tissue Reveals That circRNA2829 Regulates Chondrocyte Proliferation and Differentiation via the miR-4286-R+1/FOXO4 Axis

Yao

Jiang

Chen

et al. 2023

IJMS

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“…This technology can elucidate gene function and structure at the overall level and reveal specific biological processes and molecular mechanisms in the occurrence of diseases ( Cui et al, 2020 ; Wang Y et al, 2021 ). In previous studies, RNA sequencing has been widely used to determine the targets and pathways of drugs in disease research ( Chen et al, 2021 ; Zhou et al, 2021 ). Therefore, we aimed to detect the molecular mechanism of metformin in osteogenic differentiation by using RNA sequencing.…”

Section: Introductionmentioning

confidence: 99%

Metformin Promotes Differentiation and Attenuates H2O2-Induced Oxidative Damage of Osteoblasts via the PI3K/AKT/Nrf2/HO-1 Pathway

et al. 2022

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At present, the drug treatment of osteoporosis is mostly focused on inhibiting osteoclastogenesis, which has relatively poor effects. Metformin is a drug that can potentially promote osteogenic differentiation and improve bone mass in postmenopausal women. We aimed to detect the molecular mechanism underlying the osteogenic effect of metformin. Our study indicated that metformin obviously increased the Alkaline phosphatase activity and expression of osteogenic marker genes at the mRNA and protein levels. The PI3K/AKT signaling pathway was revealed to play an essential role in the metformin-induced osteogenic process, as shown by RNA sequencing. We added LY294002 to inhibit the PI3K/AKT pathway, and the results indicated that the osteogenic effect of metformin was also blocked. Additionally, the sequencing data also indicated oxidation-reduction reaction was involved in the osteogenic process of osteoblasts. We used H2O2 to mimic the oxidative damage of osteoblasts, but metformin could attenuate it. Antioxidative Nrf2/HO-1 pathway, regarded as the downstream of PI3K/AKT pathway, was modulated by metformin in the protective process. We also revealed that metformin could improve bone mass and oxidative level of OVX mice. In conclusion, our study revealed that metformin promoted osteogenic differentiation and H2O2-induced oxidative damage of osteoblasts via the PI3K/AKT/Nrf2/HO-1 pathway.

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“…The miR-29 family consists of miR-29a, miR-29b, and miR-29c and the expression of miR-29b-3p is upregulated in mouse and human OA cartilage [ [10] , [11] , [12] , [13] ]. Whilst TGF-β1 induces cell proliferation and accelerates the transition from G1 to S phase in the cell cycle of deer antler chondrocytes, miR-29b-3p inhibits cell proliferation and induces cell cycle arrest in rat articular chondrocytes [ 12 , 14 ]. Although miR-29b-3p has been identified as a downstream target of TGF-β1 in human OA chondrocytes, it is unknown whether miR-29b-3p can mediate TGF-β1’s ability to promote chondrocyte proliferation [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

miR-29b inhibits TGF-β1-induced cell proliferation in articular chondrocytes

Horita

Hsu

Raper

et al. 2022

Biochemistry and Biophysics Reports

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Transforming growth factor β1 (TGF-β1) is a known regulator of chondrocyte proliferation and promotes cartilage repair in osteoarthritis (OA). microRNA-29b-3p (miR-29b-3p) is downregulated by TGF-β1 and overexpressed in OA cartilage. However, the ability of miR-29b-3p to mediate the chondrocyte pro-proliferative effects of TGF-β1 is not yet understood. This current study aimed to investigate the effect of miR-29b-3p on TGF-β1-induced cell proliferation in murine articular chondrocytes. The stimulation of chondrocytes by TGF-β1 for 24 h resulted in the downregulation of miR-29b-3p expression. The ratio of G0/G1 phase cells decreased in response to TGF-β1 whereas the ratio of S phase cells was increased. Consistent with this observation, miR-29b-3p overexpression inhibited TGF-β1’s ability to promote the ratio of S phase cells and downregulate the ratio of G0/G1 phase cells. These findings suggest that the downregulation of miR-29b-3p is a likely requirement for TGF-β1-mediated proliferation of murine articular chondrocytes. Furthermore, implying that miR-29b-3p expression may be involved in reduced chondrocyte proliferation in OA.

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Effects and mechanism of lncRNA‐27785.1 that regulates TGF‐β1 of Sika deer on antler cell proliferation

Cited by 6 publications

References 43 publications

Whole-Transcriptome Sequencing of Antler Tissue Reveals That circRNA2829 Regulates Chondrocyte Proliferation and Differentiation via the miR-4286-R+1/FOXO4 Axis

Whole-Transcriptome Sequencing of Antler Tissue Reveals That circRNA2829 Regulates Chondrocyte Proliferation and Differentiation via the miR-4286-R+1/FOXO4 Axis

Metformin Promotes Differentiation and Attenuates H2O2-Induced Oxidative Damage of Osteoblasts via the PI3K/AKT/Nrf2/HO-1 Pathway

miR-29b inhibits TGF-β1-induced cell proliferation in articular chondrocytes

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