Effects and Mechanisms of Resveratrol on the Adhesion of Lactobacillus acidophilus NCFM

Abstract

Based on the adhesion and surface properties of Lactobacillus acidophilus NCFM, ve common polyphenols in fruits and vegetables were screened, and the reasons for resveratrol promoting adhesion were systematically explained. The effects of resveratrol, epicatechin, quercetin, hesperidin and caffeic acid on adhesion and surface properties of NCFM were evaluated. The results showed that resveratrol could signi cantly enhance NCFM adhesion to mucin, followed by epicatechin, caffeic acid and hesperidin, while quercetin had a certain degree of inhibition. The effects of these polyphenols on surface hydrophobicity and auto-aggregation of NCFM were consistent with adhesion results. Then, how resveratrol promotes NCFM adhesion was further explored. The results of proteomic analysis showed that resveratrol changed the surface layer proteins of NCFM, involving changes in the relative abundance of 16 proteins. In addition, resveratrol promoted the expression of mucin genes and the glycosylation mucins on the HT-29 cell surface. Our results indicate that resveratrol changes the surface layer proteins of NCFM to modify surface properties and adhere to mucins. Meanwhile resveratrol promotes expression and glycosylation of mucins in HT-29 cells. Our ndings provide theoretical support for an in-depth explanation of the interaction among resveratrol, NCFM and the HT-29 cells.

Interactions between polyphenols from Theobroma cacao and Lactobacillales to evaluate the potential of a combined strategy for intestinal free-fatty acid removal

“…However, the combination of these has the potential to provide multiple health benefits. For example, polyphenols have been observed to enhance the adhesion to intestinal mucus of Lactobacillus strains ( Yuan et al, 2022 ), both biocompounds have been observed to prevent or restore gut microbiota dysbiosis ( Li et al, 2021 ; Anjana and Tiwari, 2022 ), and both prevent and manage diabetes ( Cao et al, 2018 ; Tao et al, 2020 ), among other benefits, thus, more studies that explore if these two biocompounds can act synergistically in different conditions are needed. It is also important to screen for α- and β-hemolysis because it can be presented in bacteria belonging to this order, as was the case for strain A1 and the commercially available reference strain JBD301; the non-antibiotic multi-resistance is another important trait to evaluate and it was low in the screened strains.…”

Screening of Lactiplantibacillus plantarum 67 with Strong Adhesion to Caco-2 Cells and the Effects of Protective Agents on Its Adhesion Ability during Vacuum Freeze Drying

“…The LAB suspension was centrifuged and washed with phosphate-buffered saline (PBS) after being grown in MRS. The lipoteichoic acids, extracellular polysaccharide (EPS) and surface proteins on the surface of LAB were removed, respectively, according to the methods in the reference [13]. The adhesion capacity of LAB to Caco-2 cells was tested after removing the adhesins, and the adhesion rate considerably decreased, showing that the removed adhesin was the key adhesin [13].…”