Effects and mechanisms of resveratrol on the amelioration of oxidative stress and hepatic steatosis in KKAy mice

Abstract

BackgroundThe exact mechanism of the protective role of Resveratrol (Res) in lipid metabolism and oxidative stress is not well elucidated. The present study aimed to investigate the potential benefits and possible mechanisms of Res on the amelioration of oxidative stress and hepatic steatosis in a KKAy mouse model.MethodsA total of 30 KKAy male mice were randomly divided into three groups: a normal chow group, a low resveratrol group and a high resveratrol group. After a 12-wk study period, serum levels of TG, TC, LDL-C and HDL-C, the liver content of TG and TC, ROS, GSH, GPx, SOD and MDA levels were measured. Ectopic lipid deposition was observed in sectioned frozen liver tissues. The mRNA levels of ATGL and HSL in the liver tissues were determined via real-time PCR. Furthermore, the protein expression of p47phox, gp91phox, ATGL, HSL, Sirt1, AMPK and FOXO1 were analyzed using western blotting.ResultsFollowing Res supplementation, serum levels of TG and MDA were decreased, while the HDL-C and SOD levels were increased in KKAy mice. Furthermore, Res treatment increased GSH and GPx in liver tissues, while it decreased ROS. In addition, Res significantly reduced hepatic steatosis. After Res treatment, concentrations of p47phox (membrane) and gp91phox proteins were reduced, while p-HSL, HSL and ATGL protein expression levels were increased. Mechanistically, the levels of Sirt1, p-AMPK and p-FOXO1 expression in the liver tissues were up-regulated following supplementation with Res, and FOXO1 protein was released from the nucleus into the cytoplasm.ConclusionsRes is able to attenuate hepatic steatosis and lipid metabolic disorder and enhance the antioxidant ability in KKAy mice, possibly by up-regulating Sirt1 expression and the phosphorylation of AMPK.

Resveratrol ameliorates high glucose-induced oxidative stress injury in human umbilical vein endothelial cells by activating AMPK

“…Here, we investigated the influence of resveratrol, a natural compound with beneficial effects in many disease models (Nagai et al, 2014, Rege et al, 2014, Saldanha et al, 2013, Zhu et al, 2014, on cell viability of HG-treated HUVECs. The anticipated HG-induced suppression of HUVEC viability was restored by treatment with resveratrol, indicating the protective potential of resveratrol in HG-induced vascular cell injury.…”

“…Although the effects of resveratrol have been linked to oxidative stress (Zhu et al , 2014) and AMPK signaling in many diseases (Lea et al , 2014, Nagai et al , 2014, the exact mechanisms by which it exerts these effects remain incompletely understood. Therefore, the aim of the present study was to investigate the effect of resveratrol on HG-induced vascular injury and clarify the mechanism underlying these effects.…”

Puerarin, isolated from Pueraria lobata (Willd.), protects against diabetic nephropathy by attenuating oxidative stress

“…And the underlying mechanism may be associated with SIRT1/FOXO1 signaling pathway (Zhu et al, 2014). The research of Tan KY et al found that resveratrol, a SIRT1 activator, could attenuate mitochondrial malfunction in diabetic mice by scavenging ROS,thereby attenuating the process and minimizing the pancreatic and skeletal damage (Tan et al, 2011).…”

Hypercholesterolemia-induced ocular disorder: Ameliorating role of phytotherapy

“…Medicinal plants such as Morus alba leaves extract [82], ginger [84], nuts [85], pumpkin [86], B-glucan [87], berry fruits [88], yellow onion (Allium cepa L.) [89] and resveratrol [90] have the capability to improve hypercholesterolemia by reducing total lipids, cholesterol and triglycerides. Many of the plants contain bioactive components having a potent antioxidant activity.…”