Effects of 1,2-dibromoethane on glutathione metabolism in rat liver and kidney

Mann, Anika; Darby, F.J.

doi:10.1016/0006-2952(85)90002-4

Cited by 6 publications

(1 citation statement)

References 16 publications

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“…The major factors regulating GSH biosynthesis (reviewed in [9]) are the availability of cysteine [3,10,11] and the activity of the first enzyme of the synthesis sequence, y-glutamylcysteine synthetase (GCS) [12][13][14]. Intracellular cysteine, the content of which is relatively low (0.1-0.2 ,umol/g of liver [10,15,16]), can be derived from protein degradation, via transport across the plasma membrane or in hepatocytes, via the cystathionine pathway from methionine and serine [1 1,17,18].…”

Section: Introductionmentioning

confidence: 99%

Role of cysteine and taurine in regulating glutathione synthesis by periportal and perivenous hepatocytes

Penttilä¹

1990

Biochemical Journal

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The uptake and metabolism of 35S-labelled sulphur amino acids were compared in periportal (PP) and perivenous (PV) rat hepatocytes, isolated by digitonin/collagenase perfusion, to identify the factors underlying the previously observed [Kera, Penttilä & Lindros, Biochem. J. (1988) 254, 411-417] higher rate of GSH replenishment in PP cells. The buthionine sulphoximine-inhibitable synthesis of GSH was faster in PP than in PV hepatocytes with both cysteine (6.1 versus 5.0 mumol/h per g of cells) and methionine (4.5 versus 3.3 mumol/h per g) as well as with endogenous precursors and L-2-oxo-4-thiazolidinecarboxylate as substrates. However, the uptake of cysteine by PP cells was slower than by PV cells (8.6 versus 10.3 mumol/h per g of cells), whereas methionine was taken up at similar rates. The activity of gamma-glutamylcysteine synthetase (GCS) was slightly higher in digitonin lysates from the PP than from the PV zone. Production of sulphate, the major catabolite of [35S]cysteine sulphur, as well as incorporation of the label into protein occurred at similar rates in PP and PV cells. Taurine, on the other hand, was produced from [35S]cysteine much faster by PV than by PP cells (0.7 versus 0.1 mumol/h per g of cells). Accordingly, the taurine content of PV hepatocytes tended to be higher and to increase faster during incubation with methionine. These results imply that metabolism of taurine is highly zonated within the acinus. They also suggest that both the slightly lower GCS activity and the fast metabolism of cysteine to taurine limit the capacity of PV hepatocytes to synthesize GSH.

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Section: Introductionmentioning

confidence: 99%

Role of cysteine and taurine in regulating glutathione synthesis by periportal and perivenous hepatocytes

Penttilä¹

1990

Biochemical Journal

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Ethylene Dibromide: Toxicology and Risk Assessment

Alexeeff

Kilgore

1990

Reviews of Environmental Contamination and Toxicology

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Sublethal effects of ethylene dibromide on wound healing and morphogenesis in Hydra oligactis

Adams

Kennedy

1992

Arch. Environ. Contam. Toxicol.

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Morphogenetic activity (budding region regeneration, foot regeneration, tentacle regeneration, spikes, separation, and waist) was observed in Hydra oligactis with two tandemly arranged gastric regions (2g hydras) following exposure to a commercial ethylene dibromide (EDB) mixture. First-stage budding hydras were selected for this study. Animals were deprived of food 24 h prior to grafting and observed at 24, 48, and 72 h after grafting. The artificial pond water (APW) group was not treated with any chemicals prior to grafting. The acetone/APW group was pre-exposed to a concentration of 20 mg/L of acetone/APW 24, 48, and 72 h prior to grafting and observed at 24 h intervals for a three-day period. The EDB: acetone/APW group was exposed to a mixture containing 5 mg/L of EDB and 15 mg/L acetone for 24, 48, and 72 h prior to grafting and observed at 24-hr intervals for three days. All animals were incubated at 19 +/- 2 degrees C. Ethylene dibromide caused a significant decrease in 2 degrees foot regeneration at the 72 h observation interval for animals pre-exposed to EDB for 48 and 72 h. There was also a significant correlation between pre-exposure and a decrease in the number of 2 degrees feet regenerated. Tentacle regeneration was significantly suppressed following a 24-h pre-exposure to EDB. Waists at the graft border significantly increased at the 48 and 72 h observation times and separation of the graft border appeared at 72 h after grafting following 72 h pre-exposure of the animals to the chemical.

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Effects of 1,2-dibromoethane on glutathione metabolism in rat liver and kidney

Cited by 6 publications

References 16 publications

Role of cysteine and taurine in regulating glutathione synthesis by periportal and perivenous hepatocytes

Role of cysteine and taurine in regulating glutathione synthesis by periportal and perivenous hepatocytes

Ethylene Dibromide: Toxicology and Risk Assessment

Sublethal effects of ethylene dibromide on wound healing and morphogenesis in Hydra oligactis

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