Effects of 17β-estradiol and progesterone on the production of adipokines in differentiating 3T3-L1 adipocytes: Role of Rho-kinase

Pektaş, Mehtap; Kurt, Akif Hakan; Ün, İsmail; Tiftik, Rukiye Nalan; Büyükafşar, Kansu

doi:10.1016/j.cyto.2014.12.020

Cited by 15 publications

(19 citation statements)

References 41 publications

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“…Studies conducted in vitro on the effects of E2 on adiponectin secretion are equally conflicting. E2 causes decreased secretion of adiponectin in cell culture with murine 3T3-L1 cells (Pektaş et al 2015), but has no effect on adiponectin secretion by the human SBGS adipocyte cell line (Horenburg et al 2008). Further studies are needed to more conclusively determine the effects of E2 on adipokine secretion.…”

Section: Adiponectinmentioning

confidence: 99%

“…Stimulation of visceral and subcutaneous adipocytes in cell culture with E2 results in an increase in Ob transcript levels and leptin secretion (Machinal et al 1999). More recently, it has been discovered that in 3T3-L1 cells, E2 does not affect leptin secretion (Pektaş et al 2015). Certain leptin SNPs such as G-2548A (rs7799039) have demonstrated a relationship with serum leptin concentrations and obesity only in young females rather than males (Shahid et al 2015).…”

Section: Leptinmentioning

confidence: 99%

“…The effects of E2 on resistin secretion are as controversial as the biology of the hormone itself. E2 causes increased secretion of resistin in cell culture with 3T3-L1 cells (Pektaş et al 2015); however, administration of E2 exogenously to female rats in diestrus causes decreased resistin in visceral WAT (Caja & Puerta 2007). In mice, experiments both in vitro and in vivo have demonstrated that E2 suppresses resistin transcript levels in WAT (Huang et al 2005).…”

Section: Resistinmentioning

confidence: 99%

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The role of sex steroids in white adipose tissue adipocyte function

Newell-Fugate

2017

Reproduction

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Abstractwith the increasing knowledge that gender influences normal physiology, much biomedical research has begun to focus on the differential effects of sex on tissue function. Sexual dimorphism in mammals is due to the combined effects of both genetic and hormonal factors. Hormonal factors are mutable particularly in females in whom the estrous cycle dominates the hormonal milieu. Given the severity of the obesity epidemic and the fact that there are differences in the obesity rates in men and women, the role of sex in white adipose tissue function is being recognized as increasingly important. Although sex differences in white adipose tissue distribution are well established, the mechanisms affecting differential function of adipocytes within white adipose tissue in males and females remain largely understudied and poorly understood. One of the largest differences in the endocrine environment in males and females is the concentration of circulating androgens and estrogens. This review examines the effects of androgens and estrogens on lipolysis/lipogenesis, adipocyte differentiation, insulin sensitivity and adipokine production in adipocytes from white adipose tissue with a specific emphasis on the sexual dimorphism of adipocyte function in white adipose tissue during both health and disease.Reproduction (2017) 153 R133-R149

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Section: Adiponectinmentioning

confidence: 99%

Section: Leptinmentioning

confidence: 99%

Section: Resistinmentioning

confidence: 99%

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The role of sex steroids in white adipose tissue adipocyte function

Newell-Fugate

2017

Reproduction

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“…Therefore, an important approach in antitumor therapeutic strategies is to inhibit antioxidant systems, such catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPx), which are the primary defense lines of the cell (19). A number of studies have shown the influence of estrogen and GPER1 on antioxidant enzymes and cytokine production (20,21).…”

Section: Introductionmentioning

confidence: 99%

Oxidative/antioxidative enzyme-mediated antiproliferative and proapoptotic effects of the GPER1 agonist G-1 on lung cancer cells

2015

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Abstract. Estrogen mediates fast signal responses or transcriptional events via G protein-coupled estrogen receptor 1 (GPER1). However, there is no data on the effect of GPER1 on lung cancer cell proliferation and apoptosis. The present study aimed to analyze the anticancer effects of the GPER1 agonist G-1 on A549 human lung cancer cells. A549 cells were treated with 17β-estradiol and G-1, and cell proliferation was analyzed using MTT and WST assays. In addition, the apoptotic effects induced by G-1 were investigated using acridine orange/ethidium bromide staining. A549 cells were treated with a half maximal inhibitory concentration of G-1 for 72 h, and nitric oxide (NO) levels and superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) enzyme activities were analyzed by spectrophotometry. The results revealed that G-1 significantly decreased cell proliferation. In addition to the antiprolif erative effect of G-1, a marked increase in apoptotic activity was observed when cells were treated with 2x10-5 M G-1. Furthermore, G-1 increased NO levels, and SOD and GPx activity. These findings indicate that the GPER1 agonist G-1 is able to exert antiproliferative and proapoptotic effects on A549 cells, and that oxidant and antioxidant molecules may mediate these effects.

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“…It is possible that prolonged exposure of adipocytes to insulin may increase leptin secretion by a combination of such mechanisms. Furthermore, it has been suggested that a delayed effect of insulin treatment on leptin secretion in adipocytes is compatible with the effect being indirect 12 and additional physiological factors may be involved in vivo with strong candidates being hormonal 34 and inflammatory 35,36 influences, and a role for the hypothalamus. 23 Should the delayed effect of insulin treatment on leptin secretion in adipocytes from type 2 diabetic donors be proven in subsequent studies, the phenomenon may be analogous to the biphasic response of insulin secretion to glucose 37 where impairment of early and late phase insulin secretion leads to different clinical outcomes.…”

Section: Discussionmentioning

confidence: 96%

Enhanced leptin synthesis and secretion in vitro by human adipocytes from type 2 diabetic donors occurs only under hyperinsulinaemic conditions

Cadagan¹

2016

JDMDC

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Effects of 17β-estradiol and progesterone on the production of adipokines in differentiating 3T3-L1 adipocytes: Role of Rho-kinase

Cited by 15 publications

References 41 publications

The role of sex steroids in white adipose tissue adipocyte function

The role of sex steroids in white adipose tissue adipocyte function

Oxidative/antioxidative enzyme-mediated antiproliferative and proapoptotic effects of the GPER1 agonist G-1 on lung cancer cells

Enhanced leptin synthesis and secretion in vitro by human adipocytes from type 2 diabetic donors occurs only under hyperinsulinaemic conditions

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